A5083510911- Cancer Immunotherapy and Biomarkers
- Single-cell and spatial transcriptomics
- Cancer Genomics and Diagnostics
- Ion channel regulation and function
- Cardiac electrophysiology and arrhythmias
- Neuroscience and Neuropharmacology Research
- RNA and protein synthesis mechanisms
- Cellular transport and secretion
- Mitochondrial Function and Pathology
- Neuroscience and Neural Engineering
- Immune Cell Function and Interaction
- Trypanosoma species research and implications
- Calcium signaling and nucleotide metabolism
- Cancer Research and Treatments
- Immunotherapy and Immune Responses
- Mass Spectrometry Techniques and Applications
- Endoplasmic Reticulum Stress and Disease
- SARS-CoV-2 and COVID-19 Research
- Lipid Membrane Structure and Behavior
- Retinal Development and Disorders
- RNA Interference and Gene Delivery
- Congenital heart defects research
- Nicotinic Acetylcholine Receptors Study
- Neurogenesis and neuroplasticity mechanisms
- COVID-19 Clinical Research Studies
Weizmann Institute of Science
2006-2024
Ben-Gurion University of the Negev
1996-2023
Doublecortin (DCX) domains serve as protein-interaction platforms. Mutations in members of this protein superfamily are linked to several genetic diseases. the human DCX gene result abnormal neuronal migration, epilepsy, and mental retardation; mutations RP1 associated with a form inherited blindness, DCDC2 has been dyslectic reading disabilities. The DCX-repeat family is composed eleven paralogs mouse. Its evolution was followed across vertebrates, invertebrates, traced unicellular...
Abstract Chemokines and chemokine receptors establish a complex network modulating immune cell migration localization. These molecules were also suggested to mediate the differentiation of leukocytes; however, their intrinsic, direct regulation lymphocyte fate remained unclear. CCR2 is main receptor inducing macrophage monocyte recruitment sites inflammation, it expressed on T cells. To assess whether directly regulates responses, we followed fates CCR2−/− cells in cell–specific inflammatory...
Chronic lymphocytic leukemia (CLL) is characterized by clonal proliferation and progressive accumulation of mature B lymphocytes in the peripheral blood, lymphoid tissues, bone marrow. CLL profound immune defects leading to severe infectious complications. T cells are numerically, phenotypically, functionally highly abnormal CLL, with only limited ability exert antitumor responses. Exhaustion has also been suggested play an important role CLL-mediated cell exhaustion achieved aberrant...
In this study we demonstrate the existence of a protein with properties voltage-dependent anion channel (VDAC) in sarcoplasmic reticulum (SR) using multiple approaches as summarized following: (a) 35 and 30 kDa proteins different SR preparations, purified from other membranal systems by Ca2+/oxalate loading sedimentation through 55% sucrose, cross-react four VDAC monoclonal antibodies. (b) Amino acid sequences three peptides derived are identical to present VDAC1 isoform. (c) Similar...
AbstractThe doublecortin-like (DCX) domains serve as protein-interaction platforms. DCXtandem appear in the product of X-linked doublecortin gene, inretinitis pigmentosa –1 (RP1), well other gene products. Mutations humanDCX are associated with abnormal neuronal migration, epilepsy, and mentalretardation; mutations RP1 a form inherited blindness, whileDCDC2 has been dyslectic reading disabilities. Motivated by possibleimportance this family, thorough analysis to detect all family members...
The interaction of membrane-embedded voltage-activated potassium channels (Kv) with intracellular scaffold proteins, such as the postsynaptic density 95 (PSD-95) protein, is mediated by channel C-terminal segment. This underlies Kv clustering at unique membrane sites and important for proper assembly functioning synapse. In current study, we address molecular mechanism underlying Kv/PSD-95 interaction. We provide experimental evidence, based on hydrodynamic spectroscopic analyses, indicating...
Heat shock proteins (HSPs) provide a useful system for studying developmental patterns in the digenetic Leishmania parasites, since their expression is induced mammalian life form. Translation regulation plays key role control of protein coding genes trypanosomatids, and directed exclusively by elements 3′ untranslated region (UTR). Using sequential deletions Hsp83 UTR (888 nucleotides [nt]), we mapped 150 nt that was required, but not sufficient preferential translation reporter gene at...
To unveil novel global changes associated with corpus luteum (CL) maturation, we analyzed transcriptome data for the bovine CL on days 4 and 11, representing developing vs. mature gland. Our analyses revealed 681 differentially expressed genes (363 318 day respectively), ≥2 fold change FDR of <5%. Different gene ontology (GO) categories were represented prominently in at these stages (e.g. 4: cell cycle, chromosome, DNA metabolic process replication 11: immune response; lipid complement...
Allosteric regulation of protein function is a fundamental phenomenon major importance in many cellular processes. Such often achieved by ligand-induced conformational changes multimeric proteins that may give rise to cooperativity function. At the heart allosteric mechanisms offered account for such phenomenon, involving either concerted or sequential transitions, lie intersubunit interactions along ligation pathway protein. However, structure-function analysis homooligomeric means...
Eukaryotic initiation factor 3 (eIF3) is a multi-protein complex and key participant in the assembly of translation machinery. In mammals, eIF3 comprises 13 subunits, most which are characterized by conserved structural domains. The trypanosomatid subunits poorly conserved. Here, we identify 12 that comprise Leishmania (LeishIF3a-l) combining bioinformatics with affinity purification mass spectrometry analyses. These results highlight strong association LeishIF3 LeishIF1, LeishIF2 LeishIF5,...
<p>Differential expression analysis of rejected vs. non-rejected tumor cells.</p>
<p>Supplementary data linked to the in vivo CRISPR screen.</p>
<p>Cell types from scRNA-seq of tumors mice inoculated with rejected or non-rejected SCCs.</p>
<p>Evaluation of Mif expression in stromal cells, and its effect on patient survival.</p>
<p>Analysis of tissue microarray biopsies from cancer patients.</p>
<p>CyTOF analysis comparing tumors derived from Mif WT and KO validating the scRNAseq findings.</p>
<p>Highest Mif expression was found in tumor cells, compared to other cell types.</p>
<p>Immunopeptidomics and TCR sequencing analyses showed similar results for the rejected non-rejected SCCs.</p>
<div>Abstract<p>Low intratumor heterogeneity correlates with increased patient survival and immunotherapy response. However, even highly homogeneous tumors are variably aggressive, the immunologic factors impacting aggressiveness remain understudied. In this study, we analyzed mechanisms underlying immune escape in murine low heterogeneity. We used immunophenotyping single-cell RNA sequencing to compare temporal growth of <i>in vivo</i> transplanted, genetically...