A5101760352- Invertebrate Immune Response Mechanisms
- Heat shock proteins research
- Neurobiology and Insect Physiology Research
- Genomics and Phylogenetic Studies
- Insect Resistance and Genetics
- Lepidoptera: Biology and Taxonomy
- Oral microbiology and periodontitis research
- Enzyme Structure and Function
- Genetic diversity and population structure
- Aquaculture disease management and microbiota
- Antimicrobial Peptides and Activities
- Insect Utilization and Effects
- Streptococcal Infections and Treatments
- Infective Endocarditis Diagnosis and Management
- Protein Structure and Dynamics
- Insect symbiosis and bacterial influences
- Silk-based biomaterials and applications
- Silkworms and Sericulture Research
- Genetics, Aging, and Longevity in Model Organisms
- Inflammatory mediators and NSAID effects
- Insect and Arachnid Ecology and Behavior
- Endoplasmic Reticulum Stress and Disease
- Redox biology and oxidative stress
- RNA modifications and cancer
- Polyamine Metabolism and Applications
Bengbu Medical College
2019-2025
Anhui Agricultural University
2013-2024
Shanghai Institute of Nutrition and Health
2024
University of Chinese Academy of Sciences
2024
Hunan Normal University
2021-2022
Chinese Academy of Agricultural Sciences
2022
Institute of Plant Protection
2022
Hebei Normal University of Science and Technology
2022
Rutgers, The State University of New Jersey
2016
Stony Brook University
2008-2015
Summary Aphids secrete diverse repertoires of salivary effectors into host plant cells to promote infestation by modulating defence. The greenbug Schizaphis graminum is an important cereal aphid worldwide. However, the secreted S. are still uncharacterized. Here, 76 proteins were identified from watery saliva using transcriptome and proteome analyses. Among them, a putative effector Sg2204 was significantly up‐regulated during feeding stages, transient overexpression in Nicotiana benthamiana...
Abstract Anticancer agents act, at least in part, by inducing reactive oxygen and nitrogen species (RONS). We examined the redox effect on SW480 HT-29 colon cancer cells of four anticancer compounds, arsenic trioxide, phosphoaspirin, phosphosulindac, nitric oxide–donating aspirin (NO-ASA). All compounds inhibited growth both cell lines (IC50, 10–90 μmol/L) induced RONS detected a general molecular probe. NO-ASA, which individual (NO, H2O2, superoxide anion, peroxynitirte), apoptotic necrotic...
Abstract The serine/cysteine protease inhibitor SCCA1 (SERPINB3) is upregulated in many advanced cancers with poor prognosis, but there limited information about whether it makes functional contributions to malignancy. Here, we show that expression promoted oncogenic transformation and epithelial–mesenchymal transition (EMT) mammary epithelial cells, silencing breast cancer cells halted their proliferation. overexpression neu+ tumors increased the unfolded protein response (UPR), IL6...
Streptococcus mutans (S. mutans) contributes to caries. The biofilm formed by S. exhibits greater resistance drugs and host immune defenses than the planktonic form of bacteria. objective this study was evaluate anti-biofilm effect cordycepin from perspective metabolomics. minimum inhibitory concentration (MIC) determined antimicrobial on mutans. 24-h treated with 128 µg/mL for 10 min at 8- or 20-h time points. Biofilm biomass metabolism were assessed using crystal violet MTT assays...
Sex-determining region Y-related high mobility group box 4 (SOX4) has been proven to serve as a critical role in cancer development and progression. However, little is known about the pathological of SOX4 breast patients. The purpose this study measure expression patients explore clinical significance SOX4. Using RT-PCR Western blot, messenger RNA (mRNA) protein were measured tissues adjacent normal mammary tissues. relationship with characteristics 148 was analyzed by immunohistochemistry....
Abstract The complete mitochondrial genome (mitogenome) of Leucoma salicis (Lepidoptera: Lymantriidae) was sequenced and annotated. It is a circular molecule 15,334 bp, containing the 37 genes usually present in insect mitogenomes. All protein-coding (PCGs) are initiated by ATN codons, other than cox1 , which CGA. Three 13 PCGs had an incomplete termination codon, T or TA, while others terminated with TAA. relative synonymous codon usage consistent those published lepidopteran sequences....
Drought and high-temperature stress are the main abiotic stresses that alone or simultaneously affect yield quality of pears worldwide. However, studies on mechanisms drought resistance in remain elusive. Therefore, molecular responses Pyrus betuleafolia, widely used rootstock pear production, to high temperatures require further study. Here, drought- high-temperature-resistant seedlings were selected from many betuleafolia seedlings. The leaf samples collected before after treatment perform...
Artemia franciscana embryos undergo encystment, developmental arrest and diapause, the last characterized by profound metabolic dormancy extreme stress resistance. Encysted contain an abundant small heat shock protein termed p26, a molecular chaperone that undoubtedly has important role in development. To understand better of p26 embryos, structural functional characteristics full-length truncated expressed Escherichia coli COS-1 cells were determined. activity declined with increasing...
ObjectivesPrevious studies have reported several different plasmids that confer multidrug resistance (MDR) including to aminoglycosides. In this study, we investigated the aminoglycoside patterns for 224 Escherichia coli isolates from diseased chickens and ducks in China, characterized a novel MDR plasmid, collected prevalence data on similar plasmids.
Serine protease inhibitors play a key role in the immune system of invertebrates by controlling proteolytic cascades. Besides its importance, knowledge on functions serpins most insects is fragmentary. In present study, we identified serpin-12 from Antheraea pernyi encoding predicted 402 amino acid residue protein (Apserpin-12). We expressed recombinant Escherichia coli and purified was used for synthesis rabbit anti-Apserpin-12 polyclonal antibodies functional studies. Quantitative...
Abstract In this study, we analyzed the complete mitochondrial genome (mitogenome) of Speiredonia retorta, which is a pest and member Lepidoptera order. total, S. retorta mitogenome was found to contain 15,652 base pairs encoding 13 protein-coding genes (PCGs), 22 tRNAs, 2 rRNAs, as well an adenine (A) + thymine (T)-rich region. These findings were consistent with composition other lepidopterans, identified all PCGs beginning at ATN codons. We also that 11 terminated canonical stop codons,...
Iron (Fe) deficiency is a general stress for many horticulture crops, causing leaf chlorosis and stunted growth. The basic–helix–loop–helix (bHLH) transcription factor (TF) was reported to function in Fe absorption; however, the regulatory mechanism of bHLH genes on iron absorption remains largely unclear pear. In this study, we found that PbbHLH155 significantly induced by deficiency. Overexpression Arabidopsis thaliana pear calli increases resistance PbbHLH155-overexpressed lines exhibited...
Oviparously developing embryos of the brine shrimp, Artemia franciscana , synthesize abundant quantities a small heat shock/α‐crystallin protein, termed p26. Wild‐type p26 functions as molecular chaperone in vitro and is thought to help encysted survive severe physiological stress encountered during diapause anoxia. Full‐length truncated cDNA derivatives were generated by PCR amplification p26‐3‐6‐3, then cloned either pET21(+) or pRSETC expressed Escherichia coli BL21(DE3). All constructs...
Esterase hydrolysis of drugs can accelerate their elimination, thereby limiting efficacy. Polyethylene glycol (PEG) covalently attached to (pegylation) is known improve the efficiency many drugs. Using as a test agent novel phospho-ibuprofen (PI), we examined whether pegylation PI could abrogate its hydrolytic degradation by esterases; PI, inhibit colon cancer growth, has carboxylic ester hydrolyzable carboxylesterases (CES). We mPEG-2000 (PI-PEG) and studied stability exposing it cells...
In the present study, complete sequence of mitochondrial genome (mitogenome) Daphnis nerii (Lepidoptera: Sphingidae) is described. The mitogenome (15,247 bp) D.nerii encodes13 protein-coding genes (PCGs), 22 transfer RNA (tRNAs), two ribosomal (rRNAs) and an adenine (A) + thymine (T)-rich region. Its gene complement order similar to that other sequenced lepidopterans. 12 PCGs initiated by ATN codons except for cytochrome c oxidase subunit 1 (cox1) seemingly CGA codon as documented in insect...