A5091762003- Gut microbiota and health
- Inflammatory Bowel Disease
- Rough Sets and Fuzzy Logic
- Data Mining Algorithms and Applications
- Mycobacterium research and diagnosis
- Liver Disease Diagnosis and Treatment
- Advanced Computational Techniques and Applications
- Drug Transport and Resistance Mechanisms
- Colorectal Cancer Screening and Detection
- Metabolomics and Mass Spectrometry Studies
- Clostridium difficile and Clostridium perfringens research
- Genomics and Phylogenetic Studies
- COVID-19 Clinical Research Studies
- Biomedical Text Mining and Ontologies
- Long-Term Effects of COVID-19
- Computational Drug Discovery Methods
- Pancreatic and Hepatic Oncology Research
- Image Processing and 3D Reconstruction
- Vehicle License Plate Recognition
- Colorectal Cancer Treatments and Studies
- Microbial Community Ecology and Physiology
- Diet and metabolism studies
- Pancreatitis Pathology and Treatment
- Pharmacological Effects of Natural Compounds
- Renal Diseases and Glomerulopathies
Children's Hospital of Zhejiang University
2022-2025
Fudan University
2024-2025
Henan Province Water Conservancy Survey and Design Research
2024
East China University of Political Science and Law
2010-2023
National Clinical Research
2023
Zhejiang University
2023
Sixth Affiliated Hospital of Sun Yat-sen University
2019-2022
Sun Yat-sen University
2019-2022
Tongji University
2008-2021
Shanghai East Hospital
2018
Objective Bile acids are regulators of lipid and glucose metabolism, modulate inflammation in the liver other tissues. Primary bile such as cholic acid chenodeoxycholic (CDCA) produced liver, converted into secondary deoxycholic (DCA) lithocholic by gut microbiota. Here we investigated possible roles non-alcoholic fatty disease (NAFLD) pathogenesis impact microbiome on signalling NAFLD. Design Serum levels fibroblast growth factor 19 (FGF19), gene expression profiles compositions were...
Abstract Despite recent progress in our understanding of the association between gut microbiome and colorectal cancer (CRC), multi-kingdom dysbiosis CRC across cohorts is unexplored. We investigated four-kingdom microbiota alterations using metagenomic datasets 1,368 samples from 8 distinct geographical cohorts. Integrated analysis identified 20 archaeal, 27 bacterial, fungal 21 viral species for each single-kingdom diagnostic model. However, data revealed superior accuracy models...
Abstract Excess body weight (EBW) increases the risk of colorectal cancer (CRC) and is linked to lower colonoscopy compliance. Here, we extensively analyzed 981 metagenome samples from multiple cohorts pinpoint specific microbial signatures their potential capability distinguishing EBW patients with CRC. The gut microbiome displayed considerable variations between lean We identify 44 37 distinct multi-kingdom species differentiating CRC controls in populations, respectively. Unique...
Previous studies establish guanylate binding protein 5 (GBP5) as a driver in the development of inflammatory bowel diseases (IBDs). Here, we aim to elucidate mechanism underlying pro-inflammatory role GBP5. We observe that loss Gbp5 causes reduced colonic inflammation and decreased numbers innate lymphoid cells (ILCs) colitis mice. The transcriptional alterations observed GBP5-deficient THP-1 mirrored those triggered by STAT1 activation, leading findings GBP5 is essential for stimulated...
Dysregulated host-microbial interactions play critical roles in initiation and perpetuation of gut inflammation Crohn's disease (CD). However, the spatial distribution interaction network across intestine its accessory tissues are still elusive. Here, we profile host proteins tissue microbes 540 samples from intestinal mucosa, submucosa-muscularis-serosa, mesenteric adipose tissues, mesentery, lymph nodes 30 CD patients spatially decipher interactions. We observe aberrant antimicrobial...
Dysbiosis of gut microbial community is associated with the pathogenesis CD and may serve as a promising noninvasive diagnostic tool. We aimed to compare performances markers different biological levels by conducting multidimensional analysis on metagenomes CD. collected fecal metagenomic datasets generated from eight cohorts that altogether include 870 patients 548 healthy controls. Microbial alterations in were assessed at including species, gene, SNV level, then models constructed using...
Ulcerative colitis (UC) is considered to be closely associated with alteration of intestinal microorganisms. According the traditional Chinese medicine (TCM) theory, UC can divided into two disease syndromes called Pi-Xu-Shi-Yun (PXSY) and Da-Chang-Shi-Re (DCSR). The relationships among gut microbiota, TCM syndromes, pathogenesis have not been well investigated.To investigate role microbiota in distinction dysbiosis between PXSY DCSR syndromes.From May 2015 February 2016, patients presenting...
Abstract Background Mesenteric adipose tissue (mAT) hyperplasia, known as creeping fat is a pathologic characteristic of Crohn’s disease (CD). The reserve in surgery associated with poor prognosis CD patients, but the mechanism remains unknown. Methods microbiome, metabolome, and host transcriptome were characterized using cohort 48 patients 16 non-CD controls. Multidimensional data including 16S ribosomal RNA gene sequencing (16S rRNA), sequencing, metabolome integrated to reveal network...
Abstract The dysbiosis of the gut microbiome is one pathogenic factors nonalcoholic fatty liver disease (NAFLD) and also affects treatment intervention NAFLD. Among microbiomes, keystone species that regulate integrity stability an ecological community have become potential targets for Here, we collected stool samples from 22 patients with steatohepatitis (NASH), 25 obese patients, 16 healthy individuals New York 16S rRNA gene sequencing. An algorithm was implemented to identify based on...
Microbial signatures show remarkable potentials in predicting colorectal cancer (CRC). This study aimed to evaluate the diagnostic powers of multimodal microbial signatures, multi-kingdom species, genes, and single-nucleotide variants (SNVs) for detecting precancerous adenomas. We performed cross-cohort analyses on whole metagenome sequencing data 750 samples via xMarkerFinder identify adenoma-associated signatures. Our revealed that fungal species outperformed from other kingdoms with an...
The polymerization of halide-substituted styrenes (XSt) and their copolymerization with styrene by half-sandwich scandium catalysts without a THF ligand have been examined. THF-free aminobenzyl complex (C5Me4SiMe3)Sc(CH2C6H4NMe2-o)2 (2) in combination 1 equiv [Ph3C][B(C6F5)4] showed high activity excellent syndiotacticity for the (XSt = p-, o-, m-ClSt, p-FSt, p-BrSt, p-ISt). Despite presence electron-withdrawing halogen substituent on aromatic ring, reached up to 105 g polymer molSc–1 h–1...
Fibulin-3 gene has been identified as an antagonist of angiogenesis. We investigated the protein expression and promoter methylation status fibulin-3 in colorectal cancer (CRC) analyzed its correlation with clinicopathological factors. The study population enrolled 85 paired CRC specimens adjacent normal tissues, well 32 cases adenoma. Genomic DNA was extracted from paraffin-embedded samples using manual microdissection. Methylation-specific polymerase chain reaction (MSP) used to determine...
Multiple mechanisms for the gut microbiome contributing to pathogenesis of nonalcoholic fatty liver disease (NAFLD) have been implicated. Here, we aim investigate contribution and potential application altered bile acids (BA) metabolizing microbes in NAFLD by post hoc analysis whole metagenome sequencing (WMS) data. The discovery cohort consisted 86 well-characterized patients with biopsy-proven 38 healthy controls. Assembly-based was performed identify BA-metabolizing microbes. Statistical...
ABSTRACT The intricate interplay between host genes and intrahepatic microbes is vital in shaping the hepatic microenvironment. This study aims to elucidate how host-microbiota interactions contribute progression of nonalcoholic fatty liver disease (NAFLD). Hepatic gene microbial profiles were analyzed from 570 samples across five cohorts, including 72 control, 124 (NAFL), 143 Borderline, 231 steatohepatitis (NASH) samples. Least absolute shrinkage selection operator penalized regression...
Prior studies indicate no correlation between the gut microbes of healthy first-degree relatives (HFDRs) patients with Crohn's disease (CD) and development CD. Here, we utilize HFDRs as controls to examine microbiota metabolome in individuals active (CD-A) quiescent (CD-R) CD, thereby minimizing influence genetic environmental factors. When compared non-relative controls, use HFDR identifies fewer differential taxa. Faecalibacterium, Dorea, Fusicatenibacter are decreased CD-R, independent...
Microbial biosynthetic diversity holds immense potential for discovering natural products with therapeutic applications, yet a substantial quantity of derived from uncultivated microorganisms remains uncharacterized. The intricate nature enzymes poses major challenge in accurately predicting the chemical structures secondary metabolites solely based on genome sequences using current rule-based methods. Here, we present DeepSeMS, large language model designed to predict various microbial gene...
The gut microbiome is closely associated with pediatric Crohn's disease (CD), while the multidimensional microbial signature and their capabilities for distinguishing CD are underexplored. This study aims to characterize alterations in develop a robust classification model. A total of 1,175 fecal metagenomic sequencing samples, predominantly from three cohorts patients, were re-analyzed raw data using uniform process pipelines obtain CD, including taxonomic profiles, functional multi-type...