A5043591998- Reproductive Biology and Fertility
- Molecular Biology Techniques and Applications
- Prenatal Screening and Diagnostics
- Advanced Fluorescence Microscopy Techniques
- Birth, Development, and Health
- Adipose Tissue and Metabolism
- Renal and related cancers
- Pluripotent Stem Cells Research
- Mitochondrial Function and Pathology
- Pediatric Urology and Nephrology Studies
- Metabolism and Genetic Disorders
- Ovarian function and disorders
- Cancer, Hypoxia, and Metabolism
- Tissue Engineering and Regenerative Medicine
- Genetic Syndromes and Imprinting
- Chronic Lymphocytic Leukemia Research
- Sperm and Testicular Function
- Glycosylation and Glycoproteins Research
- Lymphoma Diagnosis and Treatment
Harvard University
2018-2024
Boston IVF
2023-2024
Universitat Autònoma de Barcelona
2019-2022
Harvard University Press
2020
Hospital Del Mar
2014
Is the combined use of fluorescence lifetime imaging microscopy (FLIM)-based metabolic and second harmonic generation (SHG) spindle a feasible safe approach for noninvasive embryo assessment?Metabolic can sensitively detect meaningful changes in embryos, SHG produces high-quality images spindles methods do not significantly impair viability.Proper metabolism is essential viability. Metabolic well-tested method measuring cells tissues, but it unclear if sensitive enough assessment.This study...
Can non-invasive imaging with fluorescence lifetime microscopy (FLIM) detect metabolic differences in euploid versus aneuploid human blastocysts?FLIM has identified significant between and blastocysts.Prior studies have demonstrated that FLIM can mouse oocytes embryos discarded blastocysts.This was a prospective observational study from August 2019 to February 2020. Embryo state assessed using measure the autofluorescence factors nicotinamide adenine dinucleotide dehydrogenase together...
Abstract STUDY QUESTION Can the BlastAssist deep learning pipeline perform comparably to or outperform human experts and embryologists at measuring interpretable, clinically relevant features of embryos in IVF? SUMMARY ANSWER The can measure a comprehensive set interpretable either these features, WHAT IS KNOWN ALREADY Some studies have applied developed ‘black-box’ algorithms predict embryo viability directly from microscope images videos but lack interpretability generalizability. Other...
Abstract STUDY QUESTION Can non-invasive metabolic imaging via fluorescence lifetime microscopy (FLIM) detect variations in profiles between discarded human blastocysts? SUMMARY ANSWER FLIM revealed extensive the state of blastocysts associated with blastocyst development over 36 h, day after fertilization and developmental stage, as well heterogeneity within individual blastocysts. WHAT IS KNOWN ALREADY Mammalian embryos undergo large changes metabolism course preimplantation development....
Deletion of 13q14 as the sole abnormality is a good prognostic marker in chronic lymphocytic leukemia (CLL). Nonetheless, value reciprocal translocations [t(13q)] with related 13q losses has not been fully elucidated. We described clinical and biological characteristics 25 CLL patients t(13q), compared 62 carrying interstitial del(13q) by conventional G‐banding cytogenetics (CGC) [i‐del(13q)] 295 only detected fluorescence situ hybridization (FISH) [F‐del(13q)]. Besides from FISH panel...
Are relative mitochondrial DNA (mtDNA) content and genome (mtGenome) variants in human cumulus cells (CCs) associated with oocyte reproductive potential assisted technology (ART) outcomes?
Can fluorescence lifetime imaging microscopy (FLIM) detect associations between the metabolic state of cumulus cell (CC) samples and clinical outcome corresponding embryos?
Abstract Study question Is there any relationship between the relative telomere length (RTL) within cumulus cells (CCs) and outcome of assisted reproductive treatment using corresponding oocyte? Summary answer Lower RTLs in CCs were significantly associated with embryos chosen for transfer or cryopreservation. In contrast, considered non-viable (discarded) tended to have higher RTLs. What is known already Cumulus fulfil vital roles support oocyte development, including transduction external...
Abstract Mammalian embryos undergo large changes in metabolism over the course of preimplantation development. Embryo has long been linked to embryo viability, suggesting its potential utility Assisted Reproductive Technologies (ART) aid selecting high quality embryos. However, human remains poorly characterized due a lack non-invasive methods measure their metabolic state. Here, we explore application imaging via fluorescence lifetime microscopy (FLIM) for studying blastocysts. We use FLIM...
Abstract Study question Can non-invasive metabolic imaging detect variations in cumulus cell parameters associated with a viable pregnancy of the corresponding embryo? Summary answer Noninvasive can differences signatures between embryos that led to and those did not. What is known already Bidirectional cooperativity human oocyte its surrounding cells essential for acquire full developmental competency. However, relationship metabolism viability not well established. Metabolic uses...