A5061724454- Trypanosoma species research and implications
- Immune Cell Function and Interaction
- HIV Research and Treatment
- COVID-19 Clinical Research Studies
- SARS-CoV-2 and COVID-19 Research
- vaccines and immunoinformatics approaches
- Cardiomyopathy and Myosin Studies
- Immunotherapy and Immune Responses
- Asthma and respiratory diseases
- Mitochondrial Function and Pathology
- Mast cells and histamine
- HIV/AIDS Research and Interventions
- Research on Leishmaniasis Studies
- Gut microbiota and health
- Toxoplasma gondii Research Studies
- Viral Infections and Immunology Research
- Immunodeficiency and Autoimmune Disorders
- T-cell and B-cell Immunology
- interferon and immune responses
- Lysosomal Storage Disorders Research
- HIV/AIDS oral health manifestations
- Immune Response and Inflammation
- Antibiotic Use and Resistance
- Vagus Nerve Stimulation Research
- Polysaccharides and Plant Cell Walls
Hospital das Clínicas da Faculdade de Medicina da Universidade de São Paulo
2018-2024
Universidade de São Paulo
2013-2023
Hospital do Coração
2022-2023
Anhembi Morumbi University
2021-2022
Microsoft (Brazil)
2021
Instituto do Coração
2021
Instituto Butantan
2017
Universidade Federal de Goiás
2015
ENT and Allergy
2011-2014
University of California, San Francisco
2013
Nicotinic α-7 acetylcholine receptor (nAChRα7) is a critical regulator of cholinergic anti-inflammatory actions in several diseases, including acute respiratory distress syndrome(ARDS). Given the potential importance α7nAChR as therapeutic target, we evaluated whether PNU-282987, an agonist, effective protecting lung against inflammation. We performed intratracheal instillation LPS to generate injury (ALI) C57BL/6 mice. PNU-282987 treatment, either before or after ALI induction, reduced...
Th9 cells orchestrate allergic lung inflammation by promoting recruitment and activation of eosinophils mast cells, stimulating epithelial mucus production, which is known to be mainly dependent on IL-9. These share developmental pathways with induced regulatory T that may determine the generation one over other subset. In fact, FOXP3 transcription factor has been shown bind il9 locus repress IL-9 production. The microbiota-derived short-chain fatty acids (SCFAs) butyrate propionate have...
Chagas disease is caused by infection with the protozoan Trypanosoma cruzi and affects over 8 million people worldwide. In spite of a powerful innate adaptive immune response in acute infection, parasite evades eradication, leading to chronic persistent low parasitism. Chronically infected subjects display differential patterns progression. While 30% develop cardiomyopathy (CCC) – severe inflammatory dilated decades after 60% patients remain disease-free, asymptomatic/indeterminate (ASY)...
Infection by the protozoan Trypanosoma cruzi causes Chagas disease cardiomyopathy (CCC) and can lead to arrhythmia, heart failure death. affects 8 million people worldwide, chronic production of cytokines IFN-γ TNF-α T cells together with mitochondrial dysfunction are important players for poor prognosis disease. Mitochondria occupy 40% cardiomyocytes volume produce 95% cellular ATP that sustain life-long cycles contraction. As have been described affect function, we hypothesized involved in...
T-cell based vaccines against HIV have the goal of limiting both transmission and disease progression by inducing broad functionally relevant T cell responses. Moreover, polyfunctional long-lived specific memory cells been associated to vaccine-induced protection. CD4+ are important for generation maintenance functional CD8+ cytotoxic cells. We recently developed a DNA vaccine encoding 18 conserved multiple HLA-DR-binding HIV-1 CD4 epitopes (HIVBr18), capable eliciting responses in HLA class...
The underlying mechanism by which MyD88 regulates the development of obesity, metainflammation, and insulin resistance (IR) remains unknown. Global deletion in high-fat diet (HFD)-fed mice resulted increased weight gain, impaired glucose homeostasis, elevated Dectin-1 expression adipose tissue (AT), proinflammatory CD11c+ AT macrophages (ATMs). KO were protected from diet-induced obesity (DIO) IR had reduced macrophages. antagonist improved homeostasis decreased chow- HFD-fed mice. agonist...
Common variable immunodeficiency (CVID) is the most prevalent symptomatic primary in adults. CVID patients often present changes frequency and function of B lymphocytes, reduced number Treg cells, chronic immune activation, recurrent infections, high incidence autoimmunity increased risk for malignancies. We hypothesized that B10 cells would be diminished because these play an important role development control T cell activation autoimmunity. Therefore, we evaluated correlated it with...
CoronaVac is an inactivated SARS-CoV-2 vaccine that has been rolled out in several low and middle-income countries including Brazil, where it was the mainstay of first wave immunization healthcare workers elderly population. We aimed to assess T cell antibody responses vaccinated individuals as compared convalescent patients. detected IgG against antigens, neutralizing antibodies reference Wuhan strain used peptides detect IFN-g IL-2 specific a group (N = 101) 72) individuals. The frequency...
ABSTRACT Background Clinical recurrence of COVID-19 in convalescent patients has been reported, which immune mechanisms have not thoroughly investigated. Presence neutralizing antibodies suggests other types response are involved. Methods We assessed the innate type I/III IFN response, T cell responses to SARS-CoV-2 with IFNγ ELISPOT, binding and antibody assays, two monozygotic twin pairs one case. Results In pair 1, four months after a first mild episode infection for both siblings,...
T-cell based vaccine approaches have emerged to counteract HIV-1/AIDS. Broad, polyfunctional and cytotoxic CD4+ responses been associated with control of HIV-1 replication, which supports the inclusion epitopes in vaccines. A successful should also be designed overcome viral genetic diversity able confer immunity a high proportion immunized individuals from diverse HLA-bearing population. In this study, we rationally multiepitopic DNA order elicit broad cross-clade against highly conserved...
Abstract Background Host–microbiota interactions shape T-cell differentiation and promote tumour immunity. Although IL-9-producing T cells have been described as potent antitumour effectors, their role in microbiota-mediated control remains unclear. Methods We analysed the impact of intestinal microbiota on colonic lamina propria germ-free dysbiotic mice. Systemic effects IL-9 were a model melanoma-challenged Results show that mice lower frequency when compared with conventional mice,...
Chronic exposure to ambient levels of air pollution induces respiratory illness exacerbation by increasing inflammatory responses and apoptotic cells in pulmonary tissues. The ineffective phagocytosis these (efferocytosis) macrophages has been considered an important factor pathological mechanisms. Depending on microenvironmental stimuli, can assume different phenotypes with functional actions. M1 are recognized their proinflammatory activity, whereas M2 play pivotal roles responding...
Abstract Cardiomyopathies are an important cause of heart failure and sudden cardiac death. Little is known about the role rare genetic variants in inflammatory cardiomyopathy. Chronic Chagas disease cardiomyopathy (CCC) prevalent Latin America, developing 30% 6 million patients chronically infected by protozoan Trypanosoma cruzi , while 60% remain free (asymptomatic (ASY)). The cytokine interferon-γ mitochondrial dysfunction to play a major pathogenetic role. provides unique model probe for...
Millions of people worldwide are currently infected with human papillomavirus (HPV), herpes simplex virus (HSV) or immunodeficiency (HIV). For this enormous contingent people, the search for preventive and therapeutic immunological approaches represents a hope eradication latent infection and/or virus-associated cancer. To date, attempts to develop vaccines against these viruses have been mainly based on monovalent concept, in which one more antigens incorporated into vaccine formulation. In...
Changing the immune responses to allergens is cornerstone of allergen immunotherapy. Allergen-specific immunotherapy that consists repeated administration increasing doses extract potentially curative. The major inconveniences allergen-specific include failure modify responses, long-term treatment leading noncompliance and potential for developing life-threating anaphylaxis. Here we investigated effect a novel liposomal formulation carrying low dose combined with CpG-ODN, synthetic TLR9...
A variety of signaling pathways are involved in the induction innate cytokines and CD8+ T cells, which major players protection against acute Trypanosoma cruzi infection. Previous data have demonstrated that a TBK-1/IRF3-dependent pathway promotes IFN-β production response to cruzi, but role for STING, main interactor these proteins, remained be addressed. Here, we STING is required IFN-β, IL-6, IL-12 infection absence negatively impacts activation IRF-dependent parasite. We reported no...
Chagas disease, caused by the protozoan Trypanosoma cruzi, is an endemic parasitic disease of Latin America, affecting 7 million people. Although most patients are asymptomatic, 30% develop complications, including often-fatal Chronic Chagasic Cardiomyopathy (CCC). previous studies have demonstrated some genetic deregulations associated with CCCs, causes their remain poorly described. Based on bulk RNA-seq and whole genome DNA methylation data, we investigated epigenetic present in moderate...
Elevated levels of immunoglobulin E (IgE) are associated with allergies and other immunological disorders. Sensitization alum adjuvant favours IgE production while CpG-ODN adjuvant, a synthetic toll-like receptor 9 (TLR9) agonist, inhibits it. The cellular mechanisms underlying in vivo TLR regulation production, specially IgE, still controversial. Specifically, TLR-mediated is not yet known. In this study we showed that augmented induced by sensitizations to OVA or without OVA-pulsed...
The COVID-19 pandemic has prompted a quest to understand why certain individuals remain uninfected or asymptomatic despite repetitive exposure SARS-CoV-2. Here, we focused on six exposed females residing with their symptomatic and reinfected SARS-CoV-2 PCR-positive partners. Peripheral blood mononuclear cell samples from couples were analysed for poly (I:C)-induced mRNA expression of type I/III interferons interferon-stimulated genes (ISGs). Remarkably, found significant upregulation the ISG...
T-cell based vaccines against SIV/HIV may reduce both transmission and disease progression by inducing broad functionally relevant T cell responses.Mounting evidence points toward a critical role for CD4 + cells in the control of immunodeficiency virus replication.We have previously shown that DNA vaccine (HIVBr18), encoding 18 HIV epitopes capable binding to multiple HLA class II molecules was able elicit broad, polyfunctional, long-lived CD8 responses BALB/c transgenic mice.By virtue...
T-cell based vaccines against human immunodeficiency virus (HIV) generate specific responses that may limit both transmission and disease progression by controlling viral load. Broad, polyfunctional, cytotoxic CD4+T-cell have been associated with control of simian virus/HIV-1 replication, supporting the inclusion CD4+ epitopes in vaccine formulations. Plasmid-encoded granulocyte-macrophage colony-stimulating factor (pGM-CSF) co-administration has shown to induce potent promote accelerated...
DNA vaccines have failed to induce satisfactory immune responses in humans. Several mechanisms of double-stranded (dsDNA) sensing been described, and modulate vaccine immunogenicity at many levels. We hypothesized that the humans is suppressed by APOBEC (apolipoprotein B (APOB) mRNA-editing, catalytic polypeptide)-mediated plasmid degradation. showed via STING (stimulator interferon (IFN) genes) TBK-1 (TANK-binding kinase 1) leads IFN-β induction, which results APOBEC3A mRNA upregulation...