A5030429120- Receptor Mechanisms and Signaling
- Nicotinic Acetylcholine Receptors Study
- Microfluidic and Capillary Electrophoresis Applications
- Gene Regulatory Network Analysis
- Pharmacological Effects and Assays
- bioluminescence and chemiluminescence research
- Biochemical Analysis and Sensing Techniques
University of Copenhagen
2022
Abstract Eukaryotic cells use G-protein coupled receptors to sense diverse signals, ranging from chemical compounds light. Here, we exploit the remarkable sensing capacity of construct yeast-based biosensors for real-life applications. To establish proof-of-concept, focus on cannabinoids because their neuromodulatory and immunomodulatory activities. We a CB 2 receptor-based biosensor, optimize it achieve high sensitivity dynamic range, prove its effectiveness in three applications increasing...
Abstract The decriminalization of cannabis and the growing interest in cannabinoids as therapeutics require efficient methods to discover novel compounds monitor cannabinoid levels human samples products. However, current are limited by structural diversity active compounds. Here, we construct a G-protein coupled receptor-based yeast whole-cell biosensor, optimize it achieve high sensitivity dynamic range, prove its effectiveness three real-life applications. First, screen library two...