A5031136757- Genetic Neurodegenerative Diseases
- Tuberculosis Research and Epidemiology
- Neuroscience and Neuropharmacology Research
- Mycobacterium research and diagnosis
- Neurological disorders and treatments
- Infectious Diseases and Tuberculosis
- Mitochondrial Function and Pathology
- Metabolomics and Mass Spectrometry Studies
- Neuroinflammation and Neurodegeneration Mechanisms
- Traditional Chinese Medicine Studies
- Immunodeficiency and Autoimmune Disorders
- Tracheal and airway disorders
- Bacterial biofilms and quorum sensing
- Analytical Chemistry and Chromatography
- Pneumocystis jirovecii pneumonia detection and treatment
- Diagnosis and treatment of tuberculosis
- Cholinesterase and Neurodegenerative Diseases
- Pneumonia and Respiratory Infections
- Child Nutrition and Feeding Issues
- Blood groups and transfusion
- Genetics, Aging, and Longevity in Model Organisms
- Viral gastroenteritis research and epidemiology
- Protein Hydrolysis and Bioactive Peptides
- Computational Drug Discovery Methods
- Dysphagia Assessment and Management
Universitat de Barcelona
2020-2024
Consorci Institut D'Investigacions Biomediques August Pi I Sunyer
2020-2024
Biomedical Research Networking Center on Neurodegenerative Diseases
2020-2024
Instituto de Salud Carlos III
2016-2024
Centro de Investigación Biomédica en Red
2024
Universitat Autònoma de Barcelona
2016-2023
Centro de Investigación Biomédica en Red de Enfermedades Respiratorias
2016-2023
Hospital Universitari Germans Trias i Pujol
2016-2023
Institut d'Investigació en Ciències de la Salut Germans Trias i Pujol
2017-2023
Departament de Salut
2020
In the last two decades, microglia have emerged as key contributors to disease progression in many neurological disorders, not only by exerting their classical immunological functions but also extremely dynamic cells with ability modulate synaptic and neural activity. This behavior, together heterogeneous roles response diverse perturbations brain parenchyma has raised idea that activation is more than anticipated understanding molecular mechanisms underlying microglial states essential...
Huntington’s disease (HD) is a neurological disorder characterized by motor disturbances. HD pathology most prominent in the striatum, central hub of basal ganglia. The cerebral cortex main striatal afferent, and progressive cortico-striatal disconnection characterizes HD. We mapped network dysfunction mice to ultimately modulate activity specific circuit ameliorate symptoms recover synaptic plasticity. Multimodal MRI vivo indicates thalamo-striatal functional deficits reduced...
Huntington's disease (HD) is an inherited neurodegenerative disorder in which the striatum most affected brain region. Although a chronic inflammatory microglial reaction that amplifies progression has been described HD patients, some murine models develop symptoms without activation. Thus, dysfunction of non-inflammatory activity could also contribute to early pathological process. Here, we show involvement microglia and particularly fractalkine signaling striatal synaptic R6/1 mice. We...
Background Smoking is a risk factor for tuberculosis (TB) infection and disease progression. Tobacco smoking increases susceptibility to TB in variety of ways, one which due reduction the IFN-γ response. Consequently, an impaired immune response could affect performance Release Assays (IGRAs). Objective In present study, we assess impact direct tobacco on radiological manifestations, sputum conversion Mycobacterium tuberculosis, analyzing secretion by IGRAs. Methods A total 525 participants...
Despite efforts to improve tuberculosis (TB) detection, limitations in access, quality and timeliness of diagnostic services low- middle-income countries are challenging for current TB diagnostics. This study aimed identify characterise a metabolic profile urine by high-field nuclear magnetic resonance (NMR) spectrometry assess whether the is also detected low-field benchtop NMR spectrometer. We included 189 patients with tuberculosis, 42 pneumococcal pneumonia, 61 individuals infected...
A quarter of the global human population is estimated to be latently infected by Mycobacterium tuberculosis (Mtb), causative agent (TB). TB remains leading cause death a single pathogen and ranks among top-10 causes overall mortality. Current immunodiagnostic tests cannot discriminate between latent, active past TB, nor predict progression latent infection disease. The only registered vaccine, Bacillus Calmette-Guérin (BCG), does not adequately prevent pulmonary in adolescents adults, thus...
ABSTRACT The alterations in the basal ganglia circuitry associated with motor symptoms Huntington’s Disease (HD) have been extensively investigated. Yet, specific contribution of direct and indirect striatal output pathways from dorsolateral (DLS) dorsomedial striatum (DMS) to dysfunction is still not fully understood. Here, using symptomatic R6/1 male mouse model HD, strong functional connectivity between DMS DLS regions rest brain were observed by fMRI, particularly pronounced DLS. Then,...
Clinical accuracy of IGRAs remains unclear on patients with immune-mediated inflammatory diseases (IMIDs). Here, we assess the impact immunosuppressants and IMIDs QuantiFERON-TB Gold In-Tube (QFN-G-IT) T-SPOT.TB accuracy.Patients who required latent tuberculosis infection (LTBI) screening were enrolled classified into: (i) 50 rheumatic diseases, (ii) psoriasis (iii) 30 Crohn's disease. A total 44 healthy individuals without immunosuppression also included as controls. Tuberculin skin test...
Aim: First, to compare in vitro minimum inhibitory concentrations (MIC) of free cloxacillin and cloxacillin-containing nanoparticles (NP) against methicillin-susceptible (MSSA) resistant Staphylococcus aureus (MRSA) second, assess NP antimicrobial activity intracellular S. aureus. Methods: Poly(d,l-lactide-co-glycolide) acid (PLGA)-NP were loaded with physico-chemically characterized. MICs determined for reference strains Newman-(MSSA) USA300-(MRSA). Murine alveolar macrophages infected,...
Interferon gamma (IFN-γ) release assays (IGRAs) are increasingly used to test for latent tuberculosis (TB) infection. Although highly specific, IGRAs have a relatively high false-negative rate in active TB patients. A more sensitive assay is needed. IFN-γ-induced protein 10 (IP-10) an alternative biomarker with 100-fold-higher expression level than IFN-γ, allowing different analysis platforms, including molecular detection. The PCR technique already integrated tool most laboratories and,...
Early and progressive cortico-striatal circuit alterations have been widely characterized in Huntington's disease (HD) patients. Cortical premotor area, M2 cortex rodents, is the most affected cortical input to striatum from early stages patients associated motor learning deficits present HD mice. Yet, sends additional long-range axon collaterals diverse output brain regions beyond basal ganglia. Here, we aimed elucidate contribution of projections pathophysiology Using fMRI, showed...
The alterations in the basal ganglia circuitry are core pathological hallmark Huntington's Disease (HD) and traditionally linked to its sever motor symptoms. Recently it was shown that optogenetic stimulation of cortical afferences striatum is able reverse symptoms HD mice. However, specific contribution direct indirect striatal output pathways from dorsolateral (DLS) dorsomedial (DMS) phenotype still not clear. Here, we aim uncover contributions these subcircuits control wild type (WT) mice...
Background: Diagnosis of nontuberculous mycobacteria (NTM) infections remains a challenge. In this study, we describe the evaluation an immunological NTM-interferon (IFN)-γ release assay (IGRA) that developed using glycopeptidolipids (GPLs) as NTM-specific antigens. Methods: We tested NTM-IGRA in 99 samples from pediatric patients. Seventy-five were patients with lymphadenitis: 25 NTM confirmed, 45 unknown etiology but compatible mycobacterial infection and 5 had lymphadenitis caused by...
Abstract Interferon (IFN)-γ release assays (IGRAs) are used to diagnose latent tuberculosis (TB) infection (LTBI). To improve the accuracy of these tests, different approaches, such as alternative cytokine detection and using antigens, considered. Following this purpose, study aims evaluate addition EspC, EspF Rv2348-B those present in QuantiFERON-TB Gold In-Tube (QFN-G-IT). We included 115 subjects: 74 active TB patients, 17 LTBI individuals 24 healthy controls. Whole blood samples were...
Loss-of-function mutations in the human vacuolar protein sorting 13 homolog A (VPS13A) gene cause Chorea-acanthocytosis (ChAc), with selective degeneration of striatum as main neuropathologic feature. Very little is known about VPS13A expression brain. The objective this work was to assess, for first time, spatiotemporal distribution mouse We found present neurons already embryonic stage, stable levels until adulthood. mRNA and distributions were similar adult a widespread distribution,...
Abstract The aim of this study was to test the use IP-10 detection in dried plasma from contact studies individuals (contacts smear positive patients), by comparing it with and IFN-γ direct plasma, establish DPS as a useful assay for LTBI diagnosis. Whole blood samples were collected 80 subjects: 12 active tuberculosis (TB), 68 studies. amount produced sensitized T cells determined QuantiFERON Gold In-Tube test. levels an in-house ELISA. For determination, two 25 µl drops Whatman903 filter...
Chorea-acanthocytosis (ChAc) is an inherited neurodegenerative movement disorder caused by VPS13A gene mutations leading to the absence of protein expression. The striatum most affected brain region in ChAc patients. However, study function has been poorly addressed. Here we generated a knockdown (KD) model and aimed elucidate contribution synaptic plasticity neuronal communication corticostriatal circuit. First, infected primary cortical neurons with miR30-shRNA against analyzed its effects...
Abstract Background Huntington's Disease (HD) is a disorder that affects body movements. Altered glutamatergic innervation of the striatum major hallmark disease. Approximately 30% those inputs come from thalamic nuclei. Foxp2 transcription factor involved in cell differentiation and reported low patients with HD. However, role thalamus HD remains unexplored. Methods We used two different mouse models HD, R6/1 HdhQ111 mice, to demonstrate consistent reduction context vivo...
RTP801/REDD1 is a stress-regulated protein whose upregulation necessary and sufficient to trigger neuronal death in vitro vivo models of Parkinson's Huntington's diseases up regulated compromised neurons human postmortem brains both neurodegenerative disorders. Indeed, disease mouse models, RTP801 knockdown alleviates motor-learning deficits.We investigated the physiological role plasticity we found rat, synapses. The absence enhanced excitatory synaptic transmission cultures brain slices...
ABSTRACT The aim of this study was to evaluate the GenoFlow DR-MTB array test (DiagCor Bioscience, Hong Kong) on 70 cultured isolates and 50 sputum specimens. showed good sensitivity specificity compared phenotypic Bactec 460TB. This accurately detected mutations in rpoB , katG inhA associated with resistance rifampin isoniazid.
Abstract Huntington’s disease (HD) is a neurological disorder characterized by motor disturbances. HD pathology most prominent in the striatum, central hub of basal ganglia. The cortex main striatal afference and progressive cortico-striatal disconnection characterizes HD. We mapped dysfunction mice to ultimately modulate activity selected circuits ameliorate symptoms recover synaptic plasticity. Multimodal MRI vivo suggested functional network deficits fronto-striatal compared...
In several low-income countries, the transport of sputa could take up to one week reach laboratories, resulting in increased contamination rates and a loss growth. The aim this study was evaluate effect OMNIgene-SPUTUM preserving Mycobacterium tuberculosis on sputum samples simulating three hypothetical scenarios for conservation and/or decontamination: (1) mixed with OMN conserved at room temperature five days then processed culture (OMN); (2) cultures followed routine standing operating...
VPS13A disease and Huntington’s (HD) are two basal ganglia disorders that may be difficult to distinguish clinically because they have similar symptoms, neuropathological features, cellular dysfunctions with selective degeneration of the medium spiny neurons striatum. However, their etiology is different. caused by a mutation in gene leading lack protein cells, while HD due an expansion CAG repeat huntingtin (Htt) gene, aberrant accumulation mutant Htt. Considering similarities both diseases...