The development of antigen-specific functional T lymphocyte immunity in infants and children is an area immunology that needs elucidation. Leukocytes from cord blood (CBL) PBL different ages who were the hospital for minor surgical procedures compared with healthy adults their ability to generate helper cell (Th) responses assessed by vitro proliferation IL-2 production after stimulation with: influenza A virus (FLU); tetanus toxoid (TET); adult allogeneic either undepleted (ALLO) or...

Multiple myeloma (MM) is characterized by the malignant expansion of differentiated plasma cells. Although many chemotherapeutic agents display cytotoxic activity toward MM cells, patients inevitably succumb to their disease because tumor cells become resistant anticancer drugs. The cancer stem cell hypothesis postulates that a small subpopulation chemotherapy-resistant responsible for propagation tumor. Herein we report efflux pluripotent dye CDy1 identifies in lines increased expression...

Summary: The Centers for Disease Control (CDC) have recently lowered the acceptable blood lead concentration in young children from ≤25 to ≤10 μg/dl (≤1.21 ≤0.48 μmol/L). incidence of toxicity with respect geographical areas and socioeconomic status has not yet been defined. We evaluated increased inner-city, suburban, rural aged 9 months 3 years. Seven hundred eighty 4,196 studied inner-city samples (18.6%) had concentrations ≥10 (0.48 μmol/L), which only 71 (1.6%) values ≥25 (1.21 Only 5...

Hemorrhage remains the leading cause of preventable death following injury. Whereas significant attention has been paid to coagulation cascade, there are fewer studies evaluating platelet dysfunction Thrombelastogram mapping (TEG-PM) allows for measurement maximal potential clot strength and selectively caused by arachidonic acid adenosine disphosphate receptors on platelet. The purpose this study was determine incidence magnitude receptor-specific injury in patients who not otherwise...

OBJECTIVE AND IMPORTANCE Granulomatous angiitis of the central nervous system is a rare cause neurological deterioration. It often diagnosed posthumously, and high index suspicion necessary to make correct diagnosis on timely basis. CLINICAL PRESENTATION A 27-year-old woman presented emergency room with complaints worsening headache, nausea, vomiting for 10 days, which were preceded by swelling her tongue. At examination, she had mild ocular tenderness, but no cranial nerve abnormalities....

Abstract Context.—Bone marrow aspirates as well bone trephine biopsies are frequently performed to assess whether there is involvement by a malignancy. Numerous reports differ in the relative value of these 2 procedures and fail provide concise guidelines that can help choose appropriate technique this clinical situation. Objective.—To compare diagnosis solid tumor metastasis Hodgkin lymphoma. In addition, we correlate our findings with those literature practice guideline. Design.—Sixty-six...

Bone marrow aspirates as well bone trephine biopsies are frequently performed to assess whether there is involvement by a malignancy. Numerous reports differ in the relative value of these 2 procedures and fail provide concise guidelines that can help choose appropriate technique this clinical situation.To compare diagnosis solid tumor metastasis Hodgkin lymphoma. In addition, we correlate our findings with those literature practice guideline.Sixty-six cases showing lymphoma aspirates,...

Background . Platelet function analysis utilizing platelet-rich plasma and optical density based aggregometry fails to identify patients at risk for uremia associated complications. Methods We employed whole blood platelet aggregation on impedance as well determination of ATP release from granules detected by a chemiluminescence method. Ten chronic kidney disease (CKD) stage 4 or 5 predialysis underwent evaluation. Our study aims evaluate this platform in patient population determine if...

Fibrinogen concentrates for use as fibrin glue were prepared by modification of a cryoprecipitate method. The goals the optimization method different centrifuges and anticoagulants assay factors not previously analyzed. Following ‐70 degrees C freeze 4 thaw, CPDA‐1 ACD plasma centrifuged at 6500 × g 5 minutes or, alternatively, 5000 7 minutes. supernatant was expressed to final volume 15.5 ± 3 mL, stored ‐30 C. Preconcentration postconcentration samples analyzed fibrinogen, fibronectin,...

Public concern about the risk of transfusion-transmitted disease, especially acquired immunodeficiency syndrome, has resulted in unprecedented growth autologous blood donation programs.1 In addition to eliminating viral diseases, use transfusion avoids sensitization red cell antigens and subsequent antibody formation. Although there are many published studies that illustrate both effectiveness safety such programs,2-4 comparatively few specifically address pediatric patient. Autologous is...

Unexplained anemia in the elderly could represent myelodysplastic syndrome (MDS). We assessed utility of using a fluorescence situ hybridization (FISH) panel for common chromosomal abnormalities seen MDS. A total 101 outpatients with unknown etiology were evaluated. Complete blood count, bone marrow biopsy, conventional cytogenetic analysis (CC), and FISH reviewed. 21 (21%) patients had combination CC identified 17 (81%) The remaining 4 (19%) diagnosed MDS based solely on morphologic...

A linear peristaltic infusion device was evaluated for red cell (RBC) transfusion in the pediatric and neonatal setting. CPDA‐1 RBC units (n = 24) divided into six groups of 4 each underwent simulated transfusion. Blood infused by using manufacturer‐provided administration sets with either a 21‐gauge needle or 24‐gauge catheter. Filters were used two to evaluate effect negative pressure on filter function. Two RBCs less than 1 week old washed, irradiated, at 5 mL per hour, standard set, 10...

We studied the usefulness of two polyester leukocyte removal depth filters for preparation leukocyte-poor platelet concentrate. The Sepacell R500A removed approximately 92% white cells and 98% platelets from stored In contrast, Travenol 4C2423 microaggregate filter less than 1% but only 14% leukocytes. These findings were obtained with (5-day-old) random donor as well 16-hour-old collected by apheresis. conclude that neither these are suitable concentrate; because it removes too many few...

Femoral hypoplasia-unusual facies syndrome comprises malformations of the skeletal system consisting shortened or absent bilateral femurs, variable bony sacral abnormalities, talipes equinovarus, and an unusual low-set ears with soft cartilage helix, up-slanting palpebral fissures, nose, blunt alae nasi, elongated phillrum, a thin upper lip. Inferiorly placed kidneys septated urinary bladder have also been reported, along cardiovascular gastrointestinal abnormalities such as esophageal...

: Thrombelastography Platelet Mapping (TEG-PM) allows for measurement of maximal potential clot strength (MA) and from stimulation arachidonic acid (MA-AA) adenosine disphosphate (MA-ADP) receptors. This study was conducted to assess degree platelet dysfunction in critically ill adult patients. A retrospective ill, adult, nontrauma patients a medical/surgical ICU August 2013 September 2014. All who underwent TEG-PM were enrolled. Patients with intracerebral hemorrhage, following cardiac...

A total of 21 neonatal infants (11 males and 10 females) were evaluated for lymphocyte subpopulation changes cellular activation following the receipt washed gamma-irradiated red cells. The mean donor exposure was 2 +/- 1.5 donors, white cell concentration each 10-mL-per-kg transfusion x 10(7) 2800 rad (28 Gy) delivered to unit prior washing. subsets CD3+, CD4+, CD8+, CD19+, CD3+/CD25+, CD3+/HLA-DR, CD4+/CD45RA, CD4+/CDw29, CD4+/ICHL-1 analyzed, as plasma gamma-interferon neopterin levels,...