There is a growing interest in the mechanisms that control apoptosis cascade during development and adult life. To investigate regulatory events trigger whole tissues, we have devised genetically encoded caspase sensor can be detected live fixed tissue by standard confocal microscopy. The comprises two fluorophores, mRFP, monomeric red fluorescent protein (mRFP) enhanced green (eGFP), are linked an efficient specific caspase-sensitive site. Upon activation, cleaved eGFP translocates to...

Tumor-host interactions play an increasingly recognized role in modulating tumor growth. Thus, understanding the nature and impact of this complex bidirectional communication is key to identifying successful anti-cancer strategies. It has been proposed that cells compete with kill neighboring host tissue clear space they can expand into; however, not demonstrated experimentally. Here we use adult fly intestine investigate existence characterize competitive tumor-host interactions. We show...

Wnt signaling is a key regulator of development that often associated with cancer. Wingless, Drosophila homolog, has been reported to be survival factor in wing imaginal discs. However, we found prospective cells survive the absence Wingless as long they are not surrounded by Wingless-responding cells. Moreover, local autonomous overactivation Wg (as result mutation APC or axin) leads elimination surrounding normal Therefore, relative differences lead competitive cell interactions. This...

Cell competition is a form of cell interaction that causes the elimination less fit cells, or losers, by wild-type (WT) influencing overall tissue health. Several mutations can cause cells to become losers; however, it not known how. Here we show Drosophila wing disc carrying functionally unrelated loser (Minute and mahjong) display common activation multiple stress signalling pathways before find these collectively account for status. We JNK inhibits growth while JAK/STAT promotes...

Throughout their lifetime, cells may suffer insults that reduce fitness and disrupt function, it is unclear how these potentially harmful are managed in adult tissues. We address this question using the Drosophila posterior midgut as a model of homeostatic tissue ribosomal Minute mutations to groups cells. take quantitative approach combining lineage tracing biophysical modeling cell competition affects stem population dynamics. show healthy induce clonal extinction weak tissues, targeting...

Tissue maintenance is underpinned by resident stem cells whose activity modulated microenvironmental cues. Using Drosophila as a simple model to identify regulators of cell behaviour and survival in vivo , we have identified novel connections between the conserved transmembrane proteoglycan Syndecan, nuclear properties function. In midgut, Syndecan depletion intestinal results their loss from tissue, impairing tissue renewal. At cellular level, alters shape, causes lamina invaginations DNA...

Epithelial homeostasis and the avoidance of diseases such as cancer require elimination defective cells by apoptosis. Here, we investigate how loss apical determinants triggers apoptosis in embryonic epidermis Drosophila. Transcriptional profiling situ hybridisation show that JNK signalling is upregulated mutants lacking Crumbs or other determinants. This leads to transcriptional activation pro-apoptotic gene reaper Suppression overexpression Puckered, a feedback inhibitor pathway, prevents...

Mechanisms communicating changes in tissue stiffness and size are particularly relevant the intestine because it is subject to constant mechanical stresses caused by peristalsis of its variable content. Using Drosophila intestinal epithelium, we investigate role vinculin, one best characterised mechanoeffectors, which functions both cadherin integrin adhesion complexes. We discovered that vinculin regulates cell fate decisions, preventing precocious activation differentiation progenitors...

Abstract The OZF (ZNF146) protein is a 33 kDa Kruppel protein, composed solely of 10 zinc finger motifs. It overexpressed in the majority pancreatic cancers and more than 80% colorectal cancers. We found an interaction between telomeric hRap1 with yeast two‐hybrid screen. (TERF2IP) ortholog scRap1 originally identified as regulator telomere length. In HeLa cells, it interacts TRF2, repeat binding factor whose inactivation causes dysregulation length structure. Immunoprecipitation anti‐hRap1...

Abstract Tissue maintenance is underpinned by resident stem cells whose activity modulated microenvironmental cues. Using Drosophila as a simple model to identify regulators of cell behaviour and survival in vivo , we have identified novel connections between the conserved transmembrane proteoglycan Syndecan, nuclear properties function. In midgut, Syndecan depletion intestinal results their loss from tissue, impairing tissue renewal. At cellular level, alters shape, causes lamina...

Abstract Mechanisms communicating changes in tissue stiffness and size are particularly relevant the intestine, because it is subject to constant mechanical stresses caused by peristalsis of its variable content. Using Drosophila intestinal epithelium, we investigate role vinculin, one best characterised mechanoeffectors, which functions both cadherin integrin adhesion complexes. We discovered that vinculin regulates cell fate decisions, preventing precocious activation differentiation...