A5091100581- Chromosomal and Genetic Variations
- Genomics and Chromatin Dynamics
- RNA and protein synthesis mechanisms
- RNA Research and Splicing
- RNA modifications and cancer
- CRISPR and Genetic Engineering
- Photosynthetic Processes and Mechanisms
- Plant Stress Responses and Tolerance
- Invertebrate Immune Response Mechanisms
- Plant Molecular Biology Research
- Enzyme-mediated dye degradation
- Developmental Biology and Gene Regulation
- Epigenetics and DNA Methylation
- Neurobiology and Insect Physiology Research
- Plant Reproductive Biology
- Advanced biosensing and bioanalysis techniques
- Legume Nitrogen Fixing Symbiosis
- Genomics and Phylogenetic Studies
- ATP Synthase and ATPases Research
- Plant nutrient uptake and metabolism
- Aluminum toxicity and tolerance in plants and animals
- Plant Virus Research Studies
- Arsenic contamination and mitigation
- Echinoderm biology and ecology
- Animal Genetics and Reproduction
University of Cambridge
2017-2025
Howard Hughes Medical Institute
2015-2018
New York University
2015-2018
Universidade Federal do Rio Grande do Sul
2011-2017
Centre National de la Recherche Scientifique
2008-2010
Institut de Biologie de l'École Normale Supérieure
2009-2010
Inserm
2010
Institut Curie
2009
Institut de Biologie Moléculaire des Plantes
2009
Genoscope
2009
Loss or gain of DNA methylation can affect gene expression and is sometimes transmitted across generations. Such epigenetic alterations are thus a possible source heritable phenotypic variation in the absence sequence change. However, attempts to assess prevalence stable natural experimental populations quantify its impact on complex traits have been hampered by confounding effects polymorphisms. To overcome this problem as much possible, two parents with little differences, but contrasting...
DNA methylation is essential for silencing transposable elements and some genes in higher eukaryotes, which suggests that this modification must be tightly controlled. However, accidental changes can transmitted through mitosis (as cancer) or meiosis, leading to epiallelic variation. We demonstrated the existence of an efficient mechanism protects against transgenerational loss Arabidopsis. Remethylation specific subset heavily methylated repeats are targeted by RNA interference (RNAi)...
PIWI proteins and their guiding Piwi-interacting small RNAs (piRNAs) are crucial for fertility transposon defense in the animal germline. In most species, majority of piRNAs produced from distinct large genomic loci, called piRNA clusters. It is assumed that germline-expressed clusters, particularly Drosophila, act as principal regulators to control transposons dispersed across genome. Here, using synteny analysis, we show clusters evolutionarily labile, arise at loci characterized by...
Abstract Background The Drosophila genus is ideal for studying genome evolution due to its relatively simple chromosome structure and small size, with rearrangements mainly restricted within arms, such as Muller elements. However, work on the rapidly evolving repetitive genomic regions, composed of transposons tandem repeats, have been hampered by lack genus-wide chromosome-level assemblies. Results Integrating long-read sequencing capture technology, here we produce annotate 30 assemblies...
Abstract The tight control of fate transitions during stem cell differentiation is essential for proper tissue development and maintenance. However, the challenges in studying sparsely distributed adult cells a systematic manner have hindered efforts to identify how multilayered regulation gene expression programs orchestrates vivo. Here, we synchronised Drosophila female germline (GSC) vivo perform in-depth transcriptome translatome analyses at high temporal resolution. This...
• Peroxidases are involved in several important processes, such as development and responses to environmental cues. In higher plants, most peroxidases encoded by large, multigenic families that mainly originated from gene chromosomal duplications. Using phylogenetic, genomic functional analyses, we have identified characterized a new class of putative heme peroxidases, called ascorbate peroxidase-related (APx-R), which arose specifically the lineage plants. The APx-R protein is structurally...
Tissue development and homeostasis depend on the balance between growth terminal differentiation, but mechanisms coordinating these processes remain elusive. Accumulating evidence indicates that ribosome biogenesis (RiBi) protein synthesis, two cellular sustaining growth, are tightly regulated yet can be uncoupled during stem cell differentiation. Using Drosophila adult female germline larval neuroblast systems, we show Mei-P26 Brat, TRIM-NHL paralogs, responsible for uncoupling RiBi...
Tissue maintenance is underpinned by resident stem cells whose activity modulated microenvironmental cues. Using Drosophila as a simple model to identify regulators of cell behaviour and survival in vivo , we have identified novel connections between the conserved transmembrane proteoglycan Syndecan, nuclear properties function. In midgut, Syndecan depletion intestinal results their loss from tissue, impairing tissue renewal. At cellular level, alters shape, causes lamina invaginations DNA...
The tight control of the mitotic phase differentiation is crucial to prevent tumourigenesis while securing tissue homeostasis. In Drosophila female germline, involves precisely four divisions, and accumulating evidence suggests that bag-of-marbles (bam), initiator differentiation, also involved in controlling number divisions. To test this hypothesis, we depleted Bam from differentiating cells found a reduced We examined regulation using RNA imaging methods bam 3’ UTR conveys instability...