A5088459735- Mycorrhizal Fungi and Plant Interactions
- Plant Pathogens and Fungal Diseases
- Fungal Biology and Applications
- Lichen and fungal ecology
- Forest Ecology and Biodiversity Studies
- Plant and Fungal Species Descriptions
- Plant Diversity and Evolution
- Plant and animal studies
- Yeasts and Rust Fungi Studies
- Insect symbiosis and bacterial influences
- Botany and Plant Ecology Studies
- Forest Insect Ecology and Management
- Studies on Chitinases and Chitosanases
- Fermentation and Sensory Analysis
- Mathematics, Computing, and Information Processing
- Plant Parasitism and Resistance
- Algal biology and biofuel production
- Infectious Diseases and Mycology
- Plant Pathogens and Resistance
- Entomopathogenic Microorganisms in Pest Control
- Invertebrate Immune Response Mechanisms
- Invertebrate Taxonomy and Ecology
- Genomics and Phylogenetic Studies
- Genetic diversity and population structure
- Silymarin and Mushroom Poisoning
Royal Ontario Museum
2015-2024
University of Toronto
2015-2024
Duke University
1999-2002
University of Pretoria
2001
National Center for Genetic Engineering and Biotechnology
2001
Louisiana State University
2001
Mount St. Joseph University
2001
National Taiwan University
1995-1996
University of Lausanne
1993-1994
ABSTRACT Fungi are an important and diverse component of soil communities, but these communities have proven difficult to study in conventional biotic surveys. We evaluated fungal diversity at two sites a temperate forest using direct isolation small-subunit internal transcribed spacer (ITS) rRNA genes by PCR high-throughput sequencing cloned fragments. identified 412 sequence types from 863 ITS sequences, as well 112 sequences other eukaryotic microorganisms. Equal proportions Basidiomycota...
P. Brandon Matheny*, Judd M. Curtisa, Valérie Hofstetterb, Catherine Aimec, Jean-Marc Moncalvod, Zai-Wei Ge, Zhu-Liang Yange, Jason C. Slotf, Joseph F. Ammiratig, Timothy J. Baronih, Neale L. Bougheri, Karen W. Hughesj, D. Jean Lodgek, Richard Kerriganl, Michelle T. Seidlm, Duur K. Aanenn, Matthew DeNitiso, Graciela Danielep, Dennis E. Desjardinq, Bradley R. Kroppr, Lorelei Norvells, Andrew Parkert, Else Vellingau, Rytas Vilgalysb & David S. Hibbettaa Biology Department, Clark University,...
Phylogenetic relationships of mushrooms and their relatives within the order Agaricales were addressed by using nuclear large subunit ribosomal DNA sequences. Approximately 900 bases 5' end nucleus-encoded RNA gene sequenced for 154 selected taxa representing most families Agaricales. Several phylogenetic methods used, including weighted equally parsimony (MP), maximum likelihood (ML), distance (NJ). The starting tree branch swapping in ML analyses was with highest score among previously...
An overview of the phylogeny Agaricales is presented based on a multilocus analysis six-gene region supermatrix. Bayesian analyses 5611 nucleotide characters rpb1, rpb1-intron 2, rpb2 and 18S, 25S, 5.8S ribosomal RNA genes recovered six major clades, which are recognized informally labeled Agaricoid, Tricholomatoid, Marasmioid, Pluteoid, Hygrophoroid Plicaturopsidoid clades. Each clade discussed in terms key morphological ecological traits. At least 11 origins ectomycorrhizal habit appear to...
Over 250 species have been described in Ganoderma. Species identification and circumscription are often unclear taxonomic segregation of the genus remains controversial. In this study we sequenced 5′ half 25S ribosomal RNA gene internal transcribed spacers to determine appropriate regions i) discriminate between Ganoderma ii) infer s. lato (Ganodermataceae) on a phylogenetic basis. We studied 19 isolates representing 14 classified 5 subgenera sections, one isolate related Amauroderma,...
The aim of the present study was to investigate phylogeny and evolution sequestrate fungi (with gastroid or partially exposed basidiomes) in relation their gilled relatives from Cortinariaceae (Basidiomycetes). Phylogenetic analyses 151 ITS sequences 77 species 37 taxa were performed using maximum parsimony likelihood methods. Results show that basidiome forms occur all three major ectomycorrhizal lineages Cortinariaceae: clades Cortinarius, Hebeloma/Hymenogaster/Naucoria, Descolea. However,...
DNA barcoding is an approach to rapidly identify species using short, standard genetic markers. The mitochondrial cytochrome oxidase I gene (COI) has been proposed as the universal barcode locus, but its utility for in mushrooms (ca. 20,000 species) not established. We succeeded generating 167 partial COI sequences (~450 bp) representing ~100 morphospecies from ~650 collections of Agaricomycotina several sets new primers. Large introns (~1500 at variable locations were detected ~5% we...
Molecular phylogenies using 1–4 gene regions and information on ecology, morphology pigment chemistry were used in a partial revision of the agaric family Hygro- phoraceae. The phylogenetically supported genera we recognize here Hygrophoraceae based these previous analyses are: Acantholichen, Ampulloclitocybe, Arrhenia, Cantharellula, Cantharocybe, Chromosera, Chrysomphalina, Cora, Corella, Cuphophyllus, Cyphellostereum, Dictyonema, Eonema, Gliophorus, Haasiella, Humidicutis, Hygroaster,...
Summary Redhead, S. A., Vilgalys, R., Moncalvo, J.‐M., Johnson, J. & Hopple, S., Jr.: Coprinus Pers. and the disposition of species sensu lato. – Taxon 50: 203–241. 2001. –1SSN 0040–0262. Based upon molecular studies, genus is subdivided into stricto ( Agaricaceae ), Coprinellus P. Karst., Coprinopsis Parasola gen. nov. in new family Psathyrellaceae. The nomenclatural history typifications names previously treated as synonyms are reviewed. It demonstrated that taxonomic characters...
Over 250 species have been described in Ganoderma. Species identification and circumscription are often unclear taxonomic segregation of the genus remains controversial. In this study we sequenced 5′ half 25S ribosomal RNA gene internal transcribed spacers to determine appropriate regions i) discriminate between Ganoderma ii) infer s. lato (Ganodermataceae) on a phylogenetic basis. We studied 19 isolates representing 14 classified 5 subgenera sections, one isolate related Amauroderma,...
We reassessed the circumscription of cantharelloid clade and identified monophyletic groups by using nLSU, nSSU, mtSSU RPB2 sequence data. Results agreed with earlier studies that placed genera Cantharellus, Craterellus, Hydnum, Clavulina, Membranomyces, Multiclavula, Sistotrema, Botryobasidium family Ceratobasidiaceae in clade. Phylogenetic analyses support monophyly all except which was highly polyphyletic. Strongly supported were: (i) Cantharellus-Craterellus, Sistotrema confluens group;...
A well‐resolved phylogeny of Flaveria is used to infer evolutionary relationships among species, biogeographical distributions, and C 4 photosynthetic evolution. Data on morphology, life history, DNA sequences (chloroplastic trnL ‐ F, nuclear ITS ETS) for 21 23 known species were collected. Each data set was analyzed separately in combination using maximum parsimony Bayesian analyses. The based the combined analysis all data. Our phylogenetic evidence indicates that 3 are basal intermediate...
We present two methods for DNA extraction from fresh and dried mushrooms that are adaptable to high-throughput sequencing initiatives, such as barcoding. Our results show these protocols yield ∼85% success recently collected materials. Tests with both recent (<2 year) older (>100 years) specimens reveal collections have low rates may be an inefficient resource populating a barcode database. However, our method of extracting herbarium samples using small amount tissue is reliable could used...
Abstract Our study evaluated in silico the potential of 14 mitochondrial genes encoding subunits respiratory chain complexes, including cytochrome c oxidase I ( CO1 ), as Basidiomycota DNA barcode. Fifteen complete and partial genomes were recovered characterized this study. Mitochondrial showed high values molecular divergence, indicating a for resolution lower‐level relationships. However, numerous introns occurred well six other genes, potentially interfering with polymerase reaction...
Recent molecular phylogenetic studies have revealed the existence of at least 50 species Morchella worldwide and demonstrated a high degree continental endemism within genus. Here we describe 19 from North America, 14 which are new (M. diminutiva, M. virginiana, esculentoides, prava, cryptica, frustrata, populiphila, sextelata, septimelata, capitata, importuna, snyderi, brunnea septentrionalis). Existing names rufobrunnea, tomentosa, punctipes angusticeps) applied to four species, formal...