A5001565009- Enzyme Structure and Function
- Protein Structure and Dynamics
- Heat shock proteins research
- Crystallization and Solubility Studies
- Freezing and Crystallization Processes
- Magnetic and Electromagnetic Effects
- Protein purification and stability
- Analytical Chemistry and Chromatography
- RNA modifications and cancer
- Cancer-related Molecular Pathways
- Neurological Disorders and Treatments
- Microfluidic and Bio-sensing Technologies
- Pharmacological Effects of Natural Compounds
- Bioactive Compounds and Antitumor Agents
- Toxin Mechanisms and Immunotoxins
- Mass Spectrometry Techniques and Applications
- Neurological Disease Mechanisms and Treatments
- Natural product bioactivities and synthesis
- Renal and related cancers
- Power Systems and Technologies
- Hydrological Forecasting Using AI
- Mesenchymal stem cell research
- Bone and Dental Protein Studies
- Phytochemical Studies and Bioactivities
- Alkaloids: synthesis and pharmacology
Xi'an Medical University
2014-2024
City University of Macau
2024
Harbin University of Commerce
2024
Shaanxi University of Chinese Medicine
2021-2024
Komatsu (Japan)
2023
Northwest University
2021-2022
Conference Board
2022
Ministry of Education of the People's Republic of China
2021
Northwestern Polytechnical University
2012-2016
China XD Group (China)
2015
The effect of magnetic fields on water is still a highly controversial topic despite the vast amount research devoted to this in past decades. Enhanced evaporation field, however, less disputed. underlying mechanism for phenomenon has been investigated previous studies. In paper, we present an investigation large gradient field. pure at simulated gravity positions (0 level (ab. g), 1 g, 1.56 g and 1.96 g) superconducting magnet was compared with that absence results showed indeed faster...
Abstract Background and Objective Octamer‐4 (Oct4), a transcription factor involved in regulating human embryonic stem cells (ESCs), may play role tumorigenesis. Since little is known about the efficacy of Oct4 as potential biomarker for gastric cancer (GC), we investigated its expression GC tissues relationship to various clinicopathological parameters. Methods Primary tumor matching, adjacent non‐cancerous were obtained from 62 patients, was examined by reverse transcription‐PCR (RT‐PCR)...
The mechanism of cross-linked protein crystals using glutaraldehyde, and their properties applications are discussed in detail.
In the present study, we investigated therapeutic potential of mesenchymal stem cells (MSCs) in a rat acute renal failure (ARF) model and explored possible vivo vitro mechanisms action. Rat human MSCs were isolated from bone marrow. After being co-cultured with injured kidney tissues trans-well dishes vitro, became rounded tubular epithelial-like cells, highly expressed markers such as cytokeratin 18 (CK18) aquaporin-1 (AQP1). Human infused into rats ARF, techniques microscopy, histology,...
This paper reports on an ultrasonic levitation system developed for crystallization from solution in a containerless condition. The has been proven to be able levitate droplets stably and grow crystals rapidly freely levitated droplet. Crystals of four samples, including NaCl, NH4Cl, lysozyme, proteinase K, were obtained successfully utilizing the system. studies showed that acoustically all exhibited higher growth rates, larger sizes, better shapes, fewer crystals, as well twins shards,...
High-quality protein crystals of suitable size are an important prerequisite for applying X-ray crystallography to determine the 3-dimensional structure proteins. However, it is often difficult obtain appropriate and quality because nucleation growth processes can be unsuccessful. Here, we show that by adsorbing proteins onto porous polystyrene-divinylbenzene microspheres (SDB) floating on surface crystallisation solution, a localised high supersaturation region at low below coexist in...
High-quality crystals are key to obtaining accurate three-dimensional structures of proteins using X-ray diffraction techniques. However, such protein is often a challenge. Several containerless crystallization techniques have been reported the ability improve crystal quality, but it unknown which most favourable way grow high-quality crystals. In this paper, quality comparison were grown under three conditions provided by diamagnetic levitation, silicone oil and agarose gel was conducted. A...
Most protein crystallisation begins from heterogeneous nucleation; in practice, typically occurs the presence of a solid surface solution. The provides nucleation site such that energy barrier for is lower on than bulk Different types surfaces exhibit different energies, and barriers depend characteristics surfaces. Therefore, treatment may alter properties to increase chance obtain crystals. In this paper, we propose method modify glass cover slip using self-assembled monolayer (SAM)...
New targeted chemotherapy agents greatly improved five-year survival in NSCLC patients, but which were susceptible to drug resistance. NVP-AUY922, terminated phase II clinical trials, exhibited promising anti-NSCLC (non-small-cell lung cancer) activity targeting Hsp90N (heat shock protein), demonstrated advantages overcoming resistance as a broad-spectrum anti-cancer target. It was expected develop novel drugs overcome by the structural optimization of NVP-AUY922. However, absence...
Nε-carboxymethyl-lysine (CML), an advanced glycation end product, is involved in vascular calcification (VC) diabetic atherosclerosis. This study aimed to investigate the effects of CML on VC atherosclerosis induced by smooth muscle cell (VSMC)-derived foam cells. Human studies, animal studies and were performed. The human results from 100 patients revealed a poor blood glucose lipid status more severe coronary lesions stenosis with artery disease diabetes mellitus. Intraperitoneal injection...
SNX-2112, as a promising anticancer lead compound targeting heat shock protein 90 (Hsp90), absence of complex crystal structure Hsp90 N -SNX-2112 hindered further structural optimization and understanding on molecular interaction mechanism. Herein, high-resolution was successfully determined by X-ray diffraction, resolution limit, 2.14 Å, PDB ID 6LTK, their analyzed in detail, which suggested that SNX-2112 well accommodated the ATP-binding pocket to disable chaperone activity Hsp90,...
We present a new method for the realization of high-throughput protein crystallization screening using an array 96 capillaries aligned in circle. In this method, each capillary represents single condition, and all experience identical magnetic field condition.
This report describes the modification of crystallization plates by simply oxidizing surface protein wells. The oxidized were tested in standard screening and reproducibility studies. results showed that wells treated smoother more optically transparent than those untreated plates, importantly, was significantly promoted after oxidation treatment. Because there is no change to routine protocol, this method simple easy apply crystallization.