A5100646968- Cancer-related molecular mechanisms research
- Circular RNAs in diseases
- MicroRNA in disease regulation
- Extracellular vesicles in disease
- RNA modifications and cancer
- Pancreatic and Hepatic Oncology Research
- Single-cell and spatial transcriptomics
- Cancer Cells and Metastasis
- Systemic Lupus Erythematosus Research
- Immune cells in cancer
- Esophageal Cancer Research and Treatment
- Cancer-related gene regulation
- Renal Diseases and Glomerulopathies
- Cancer Genomics and Diagnostics
- Inflammatory Biomarkers in Disease Prognosis
- Lipid metabolism and disorders
- Systemic Sclerosis and Related Diseases
- Cancer, Lipids, and Metabolism
- Kruppel-like factors research
- Peptidase Inhibition and Analysis
- Genetic factors in colorectal cancer
- Vasculitis and related conditions
- TGF-β signaling in diseases
- Nerve injury and regeneration
- HVDC Systems and Fault Protection
Shandong First Medical University
2018-2025
Shandong Provincial Hospital
2017-2025
Central South University
2020-2024
Second Xiangya Hospital of Central South University
2020-2024
Liaocheng People's Hospital
2024
Central Hospital of Wuhan
2024
Hubei University of Arts and Science
2024
Xiangyang Central Hospital
2024
Hunan Provincial Center for Disease Control and Prevention
2023
Longhua Hospital Shanghai University of Traditional Chinese Medicine
2020-2022
Chromatin accessibility plays a crucial role in mediating transcriptional dysregulation and heterogeneity Esophageal Squamous Cell Carcinoma (ESCC). Examining the chromatin of ESCC at single-cell level is imperative to understand how it activates oncogenes contributes onset metastasis ESCC. We performed assay for transposase-accessible sequencing (scATAC-seq) on cancerous adjacent noncancerous tissues from four patients who were pathological staged as T1a, T2b, T3b, or T4a, investigate...
Myocardial infarction associated transcript (MIAT) is identified as a long chain non-coding RNA (lncRNA), which was with myocardial susceptibility. While intense efforts have been made to elucidate the relationship between MIAT and carcinogenesis, tumor immunoreaction of remains elusive. Thus, this study aimed investigate role in immunoregulation breast cancer (BC) further explore better clinical significance.The differential expression BC normal/adjacent tissues compared using Wilcoxon rank...
Oncolytic virotherapies have emerged as new modalities for cancer treatment. We previously reported that coxsackievirus B3 (CVB3) is a novel oncolytic virus (OV) with strong ability to lyse human non-small cell lung cells; however, its non-specific toxicity against normal cells remains be resolved. To improve safety profile, microRNA target sequences complementary miR-34a/c, which expressed preferentially in cells, were inserted into the 5' UTR or 3' of CVB3 genome. In presence gene-modified...
The underlying circular RNAs (circRNAs)-related competitive endogenous RNA (ceRNA) mechanisms of pathogenesis and prognosis in non-small cell lung cancer (NSCLC) remain unclear.Differentially expressed circRNAs (DECs) two Gene Expression Omnibus datasets (GSE101684 GSE112214) were identified by utilizing R package (Limma). Circinteractome StarBase databases used to predict circRNA associated-miRNAs mRNAs, respectively. Then, protein-protein interaction (PPI) network hub genes ceRNA...
Understanding the regulatory mechanisms within esophageal epithelia is essential to gain insight into pathogenesis of diseases, which are among leading causes morbidity and mortality throughout world. The zinc-finger transcription factor Krüppel-like (KLF4) implicated in a large number cellular processes, such as proliferation, differentiation, inflammation epithelia. In murine epithelia, Klf4 overexpression chronic mediated by activation NFκB signaling downstream KLF4, this produces...
Background Previous studies have elucidated that FOXM1 may predict poor prognosis in patients with multiple solid malignant tumors. In this study we explored the differential expression of stage IIA esophageal squamous cell carcinoma (ESCC) and investigated its prognostic value. Methods Immunohistochemistry (IHC) Western blot were used to detect ESCC. Correlations between clinicopathological variables, five‐year lymphatic metastatic recurrence (LMR) overall survival (OS) analyzed. Results...
Enhancing the potency of dendritic cells (DCs) by downregulating negative immunoregulatory pathways may provide immunotherapeutic possibilities against candidiasis.In this study, a si-RNA method is used to repress expression cytokine signaling-3 suppressor (SOCS3) in murine bone marrow-DCs, and then maturation DCs subsequent T-cell response after exposure C. albicans are monitored vitro.Along with higher DC markers CD40, CD86 MHC-II, IL-6/STAT3 markedly upregulated SOCS3 siRNA-treated as...
Bone tissue is a common metastatic site of lung cancer, and bone metastasis characterized by abnormal differentiation malfunction osteoclast, the roles exosomes derived from cancer have attracted much attention. In our study, we found that level HOTAIR expression in A549 H1299 was higher than those normal fibrocytes. Overexpression promoted osteoclast differentiation. Furthermore, A549-Exos H1299-Exos targeted tissues, formation significantly inhibited vivo. Mechanistically, exosomal lncRNA...
Osseous metastases of pulmonary carcinoma and the detailed mechanisms remain unclear, effects exosomes (Exos) originated from adenocarcinoma cells in this process have received a lot attentions. Our study revealed that Exos secreted A549 (A549-Exos) enhanced osteoclastogenesis osseous resorption vitro. In addition, A549-Exos showed targeted effect on bones to enhance vivo. A549-exosomal miR-328 via downregulating neuropilin 2 (Nrp-2) expression, inhibitors suppressed Therefore, enhances...
To investigate whether FGFR1 gene amplification is associated with clinicopathologic characteristics and its potential impact on survival in patients resected esophageal squamous cell carcinoma (ESCC).Five hundred fifty-six ESCC undergoing curative resection of were retrospectively studied. copy number was determined microarrayed tumor samples using fluorescent situ hybridization (FISH) analysis. status prespecified as ≥ 6 or FGFR1/CEN 8 ratio 2.2. expression evaluated by...
Runt‑related transcription factor 3 (RUNX3) is a candidate tumor suppressor, and its inactivation may play crucial role in the carcinogenesis process of numerous cancer types, including esophageal squamous cell carcinoma (ESCC). We previously revealed that RUNX3 was correlated with lymph node metastasis (LNM) ESCC recurrence. However, exact mechanisms this are still under investigation. The aim present study to examine potential roles underlying molecular epithelial‑mesenchymal transition...
Ras homolog enriched in brain (Rheb) is a small GTPase that activates mammalian target of rapamycin complex 1 (mTORC1). Previous studies have shown constitutively active Rheb can enhance the regeneration sensory axons after spinal cord injury by activating downstream effectors mTOR. S6K1 and 4E-BP1 are important mTORC1. In this study, we investigated role Rheb/mTOR its protection retinal ganglion cells. We transfected an optic nerve crush mouse model with adeno-associated viral 2-mediated...
Our study is aimed at investigating whether Lipopolysaccharide- (LPS-) treated podocytes could polarize naive CD4+ T cells into different subsets in vitro.Podocytes and mouse bone marrow-derived dendritic (BMDCs) were first cultured with 25 μg/ml LPS for 6 hours, respectively. Then, cocultured the LPS-treated or BMDCs a ratio of 1 : 1. After 48 we collected suspended supernatant from all groups to measure helper (Th)17 cells, regulatory (Treg) cytokine concentration.We observed expression...