Expansins are plant cell wall loosening proteins that involved in enlargement and a variety of other developmental processes. The expansin superfamily contains four subfamilies; namely, α-expansin (EXPA), β-expansin (EXPB), expansin-like A (EXLA), B (EXLB). Although the genome sequencing soybeans is complete, our knowledge about pattern expansion evolutionary history soybean genes remains limited. total 75 were identified genome, grouped into subfamilies based on their phylogenetic...

Abstract Background WRKY genes encode one of the most abundant groups transcription factors in higher plants, and its members regulate important biological process such as growth, development, responses to biotic abiotic stresses. Although soybean genome sequence has been published, functional studies on still lag behind those other species. Results We identified a total 133 genome. According structural features their encoded proteins phylogenetic tree, family could be classified into three...

Salinity is a major abiotic stress affecting plant growth and development. Understanding the molecular mechanisms of salt response defense in plants will help efforts to improve tolerance crops. Brachypodium distachyon new model for wheat, barley, several potential biofuel grasses. In current study, proteome phosphoproteome changes induced by were focus. The Bd21 leaves initially treated with concentrations ranging from 80 320 mm then underwent recovery process prior analysis. A total...

Expansin loosens plant cell walls and involves in enlargement various abiotic stresses. Plant expansin superfamily contains four subfamilies: α-expansin (EXPA), β-expansin (EXPB), expansin-like A (EXLA), B (EXLB). In this work, we performed a comprehensive study on the molecular characterization, phylogenetic relationship expression profiling of common wheat (Triticum aestivum) gene family using recently released genome database (IWGSC RefSeq v1.1 with coverage rate 94%).Genome-wide analysis...

Abstract Background Low-molecular-weight glutenin subunits (LMW-GS) play a crucial role in determining end-use quality of common wheat by influencing the viscoelastic properties dough. Four different methods - sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), two-dimensional (2-DE, IEF × SDS-PAGE), matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) and polymerase chain reaction (PCR), were used to characterize LMW-GS composition...

High molecular weight glutenin subunits (HMW-GS) from 60 germplasms including 30 common wheat cultivars and related species were separated characterized by a suite of separation methods sodium dodecyl sulfate−polyacrylamide gel electrophoresis (SDS-PAGE), reversed-phase high-performance liquid chromatography (RP-HPLC), capillary (HPCE), matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS). Comparative analysis demonstrated that each methodology has its...

Multidrug and toxic compound extrusion (MATE) transporter proteins are present in all organisms. Although the functions of some MATE gene family members have been studied plants, few studies investigated expansion patterns, functional divergence, or effects positive selection.Forty-five genes from rice 56 Arabidopsis were identified grouped into four subfamilies. similar exon-intron structures Arabidopsis; conserved each subfamily but differ among In both species, has expanded mainly through...

A wheat cultivar “Chinese Spring” chromosome substitution line CS-1Sl(1B), in which the 1B was substituted by 1Sl from Aegilops longissima, developed and found to possess superior dough breadmaking quality. The molecular mechanism of its super quality conformation is studied aspects high glutenin genes, protein accumulation patterns, polymeric proteins, bodies, starch granules, disulfide isomerase (PDI) PDI-like expressions. Results showed that introduced HMW-GS 1Sl×2.3* 1Sly16* possesses...

Abstract Protein acetylation and succinylation are the most crucial protein post-translational modifications (PTMs) involved in regulation of plant growth development. In this study, we present first lysine-acetylation lysine-succinylation proteome analysis seedling leaves Brachypodium distachyon L (Bd). Using high accuracy nano LC-MS/MS combined with affinity purification, identified a total 636 lysine-acetylated sites 353 proteins 605 lysine-succinylated 262 proteins. These participated...

DNA binding with one finger (Dof) transcription factors play important roles in plant growth and abiotic stress responses. Although genome-wide identification analysis of the DOF factor family has been reported other species, no relevant studies have emerged wheat. The aim this study was to investigate evolutionary functional characteristics associated responses by wheat Dof gene family.Using recently released genome database (IWGSC RefSeq v1.0), we identified 96 members, which were...

The Multidrug and toxin efflux (MATE) gene family plays crucial roles in plant growth development response to adverse stresses. This work investigated the structural evolutionary characteristics, expression profiling potential functions involved aluminium (Al) tolerance from a genome-wide level. In total, 211 wheat MATE genes were identified, which classified into four subfamilies unevenly distributed on chromosomes. Duplication analysis showed that fragments tandem repeats played main...

Abstract Background Multiple proteins containing BURP domain have been identified in many different plant species, but not any other organisms. To date, the molecular function of is still unknown, and no systematic analysis expression profiling gene family soybean ( Glycine max ) has reported. Results In this study, multiple bioinformatics approaches were employed to identify all members genes soybean. A total 23 types identified. These had diverse structures distributed on chromosome 1, 2,...

The 14-3-3 gene family identified in all eukaryotic organisms is involved a wide range of biological processes, particularly resistance to various abiotic stresses. Here, we performed the first comprehensive study on molecular characterisation, phylogenetics and responses stresses Brachypodium distachyon L.. A total seven genes from B. 120 five main lineages among 12 species were identified, which divided into well-conserved subfamilies. structure analysis showed that plant highly...

GATA transcription factor genes participate in plant growth, development, morphogenesis, and stress response. In this study, we carried out a comprehensive genome-wide analysis of wheat to reveal their molecular evolutionary characteristics involvement salt drought tolerance. total, 79 TaGATA containing conserved domain were identified the genome, which classified into four subfamilies. Collinear indicated that fragment duplication plays an important role amplification gene family....

The auxin response factor (ARF) gene family is involved in plant development and hormone regulation. Although the ARF has been studied some species, its structural features, molecular evolution, expression profiling Brachypodium distachyon L. are still not clear.Genome-wide analysis identified 19 genes B. distachyon. A phylogenetic tree constructed with 182 from seven species revealed three different clades, within a clade exhibited conservation, although certain divergences occurred clades....

NAC (NAM, ATAF1/2, CUC2) transcription factors are involved in regulating plant developmental processes and response to environmental stresses. Brachypodium distachyon is an emerging model system for cereals, temperate grasses biofuel crops. In this study, a comprehensive investigation of the molecular characterizations, phylogenetics expression profiles under various abiotic stresses gene family was performed. total, 118 BNAC genes B. were identified, which 22 (18.64%) tandemly duplicated...

Cadmium, one of the toxic heavy metals, robustly impact crop growth and development food safety. In this study, mechanisms wheat (Triticum aestivum L.) selenium-binding protein-A (TaSBP-A) involved in response to Cd stress was fully investigated by overexpression Arabidopsis wheat. As a cytoplasm protein, TaSBP-A showed high expression plant roots its levels were highly induced treatment. The enhanced Cd-toleration yeast, Meanwhile, transgenic under lower H2O2 malondialdehyde content higher...

Two new x-type high-molecular-weight glutenin subunits with similar size to 1Dx5, designated 1Dx5*t and 1Dx5.1*t in Aegilops tauschii, were identified by SDS-PAGE, RP-HPLC, MALDI-TOF-MS. The coding sequences isolated AS-PCR the complete ORFs obtained. Allele consists of 2481 bp encoding a mature protein 827 residues deduced Mr 85,782 Da whereas comprises 2526 842 87,663 Da. Mr's both genes consistent those determined Molecular structure analysis showed that repeat motifs correspondingly...

Protein disulfide isomerases (PDI) are involved in catalyzing protein bonding and isomerization the endoplasmic reticulum functions as a chaperone to inhibit aggregation of misfolded proteins. Brachypodium distachyon is widely used model plant for temperate grass species such wheat barley. In this work, we report first molecular characterization, phylogenies, expression profiles PDI PDI-like (PDIL) genes B. different tissues under various abiotic stresses. Eleven PDIL genome by silico...

Protein phosphorylation is one of the most important post-translational modifications involved in regulation plant growth and development as well diverse stress response. As a member Poaceae, Brachypodium distachyon L. new model for wheat barley several potential biofuel grasses such switchgrass. Vegetative vital biomass accumulation plants, but knowledge regarding role protein modification during vegetative growth, especially far from comprehensive. In this study, we carried out first...