In previous studies we reported that plasmacytoid dendritic cells (PDC) infiltrating head and neck cancer tissue are functionally impaired, but the molecular basis for functional deficiency remained unclear. Here demonstrate tumour-derived prostaglandin E2 (PGE(2)) transforming growth factor-beta (TGF-beta) increase interleukin-8 (IL-8) synergistically inhibit interferon-alpha (IFN-alpha) tumour necrosis factor (TNF) production of Toll-like receptor 7 (TLR7)- 9 (TLR9)-stimulated PDC. The...

// Verena Rolfes 1, * , Christian Idel 2, Ralph Pries Kirstin Plötze-Martin 2 Jens Habermann 3 Timo Gemoll Sabine Bohnet 4 Eicke Latz 5, 6 Julika Ribbat-Idel 7 Bernardo S. Franklin and Barbara Wollenberg 1 Institute of Innate Immunity, University Hospital, Bonn, Germany Hospital Schleswig Holstein, Campus Lübeck, Clinic for Otorhinolaryngology – Head Neck Surgery, Luebeck, Section Translational Oncology Biobanking, Pulmonary Medicine, 5 Department Infectious Diseases...

Abstract Bisphosphonates are frequently used for the antiresorptive treatment in bone metastasis diseases or osteoporosis. A side effect of this therapy is osteonecrosis jaw. This inhibits osteoclast function, but osteoblasts and fibroblasts also negatively affected terms impaired proliferation. Additive local with platelet-rich plasma (PRP) fibrin (PRF) promotes adhesion, proliferation migration cells due to high concentrations growth factors like PDGF, TGF IGF. The aim study was...

The T helper 17 (Th17) cells recently identified as distinct cell lineage are characterised by their production of the proinflammatory cytokine interleukin 17. Although much effort has been made in understanding function Th17 pathogenesis different diseases, influence carcinogenesis remain largely unknown.We studied prevalence and induction head neck squamous carcinoma (HNSCC) patients flow cytometry. To determine migration mechanism into primary tumours metastasis HNSCC, we performed...

In head and neck squamous cell carcinoma (HNSCC) aerobic glycolysis is the key feature for energy supply of tumor. Quantitative microdialysis (μD) offers an online method to measure parameters carbohydrate metabolism in vivo. The aim was standardize a quantitative μD-study patients with HNSCC prove if ketogenic diet would differently influence tumor tissue. Commercially available 100 kDa-CMA71-μD- catheters were implanted tumor-free tissue simultaneous measurements up 5 days. metabolic...

Background: As a tomographic imaging technology, magnetic particle (MPI) allows high spatial resolution and sensitivity, the possibility to create real-time images by determining distribution of particles. To ensure prospective biosafe application UL-D (University Luebeck-Dextran coated superparamagnetic nanoparticles), we evaluated biocompatibility iron oxide nanoparticles (SPIONs), their impact on biological properties, cellular uptake using head neck squamous cancer cells (HNSCCs)....

Obstructive sleep apnea syndrome (OSAS) represents a substantial disease of recurrent fragmentation, leading to intermittent hypoxia and subsequent diseases such as cardiovascular, metabolic, or cognitive dysfunctions. In addition, OSAS is considered low-grade systemic inflammation, which associated with higher incidence cancer, severity infections, an overall immune dysregulation. This research project aims comprehensively investigate the interplay wholesome functions circulating monocytes...

Chronic Otitis Media (COM) is defined as long term inflammation and colonization with pathogenic bacteria due to a defect or retraction of the tympanic membrane. Surgical interventions are often augmented by antibiotic resistance development therefore, off-label treatment using natural drug 1,8-Cineol was carried out. All COM patients underwent therapy middle ear surgery developed resistances. Microbiological investigations from auditory canal stool samples were performed in correlation...

ABSTRACT The role of plasmacytoid dendritic cells (PDC), the major producers alpha interferon upon viral infection, in nasal mucosa is largely unknown. Here we examined presence PDC together with myeloid (MDC) epithelia healthy individuals, asymptomatic patients chronic allergy, undergoing steroid therapy, and infectious rhinitis or rhinosinusitis. Considerable numbers MDC could be detected epithelium. Furthermore, demonstrate expression SDF-1, chemoattractant for PDC, levels were...

Protein expression of human toll-like receptors (TLR) 1-10 was measured in cell lines and solid tumors head neck squamous carcinoma (HNSCC). All HNSCC 80% were found to express TLR3 as a predominantly intracellular protein, while no other TLR proteins expressed. has previously been shown contribute the activation nuclear factor-kappaB (NF-kappaB), transcription factor which promotes several types cancers. Significantly, NF-kappaB strongest protein extracts from tissue overexpressed....

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Head and neck squamous cell carcinoma (HNSCC) is an aggressive epithelial malignancy of the upper aerodigestive tract, associated with poor survival. As part HNSCC microenvironment, interleukin-18 (IL-18)/IL-18 binding protein (IL-18BP) signaling becoming increasingly interesting as a potential biomarker therapeutic target. However, systemic expression levels IL-18BP in context immunological environment patients remain unexplored. ELISA measurements plasma IL-18-BP were carried out regard to...

Abstract 1,8-Cineol is a natural plant-based therapeutic agent and commonly used to treat broad range of acute chronic airway inflammatory diseases. has recently been shown attenuate the checkpoint molecule PDL-1 in circulating monocytes patients with Otitis media (OM) was associated an improved clinical outcome. Hypoxia-inducible factor (HIF) thought play essential role middle ear process, mainly due dysfunctions eustachian tube. However, unambiguous impact on hypoxia-driven immune...

Lung cancer remains one of the most prevalent cancers worldwide, with high morbidity and mortality rates. Besides established treatment options such as surgery radio(chemo)therapy, advanced approaches anti-programmed death 1 (PD-1)/anti-programmed ligand (PD-L1) immune checkpoint inhibitors (ICIs) have been introduced effective therapeutic options. In this context, PD-L1 expression on myeloid cells has correlated poor clinical outcomes in patients cancer. This study aimed to investigate...

It has been shown that head and neck squamous cell carcinoma (HNSCC) are infiltrated by plasmacytoid dendritic cells (pDCs). The HNSCC TH2 biased microenvironment leads to strong alterations of the cellular functions pDC thus impairs initiation function adequate immune responses. In this work we comprehensively analyzed capacity CpG-oligonucleotides activate interferon (IFN)-α secretion human in presence HNSCC. IFN-α was measured using ELISA Technique. Class A CpG dinucleotide 2216 used...