A5101616295- RNA modifications and cancer
- Cancer-related molecular mechanisms research
- MicroRNA in disease regulation
- RNA Research and Splicing
- Metal complexes synthesis and properties
- Cancer, Hypoxia, and Metabolism
- Erythrocyte Function and Pathophysiology
- Cancer Research and Treatments
- Thyroid Disorders and Treatments
- Endoplasmic Reticulum Stress and Disease
- Esophageal Cancer Research and Treatment
- Peroxisome Proliferator-Activated Receptors
- Petroleum Processing and Analysis
- Glutathione Transferases and Polymorphisms
- Wheat and Barley Genetics and Pathology
- bioluminescence and chemiluminescence research
- Heat transfer and supercritical fluids
- Fungal Biology and Applications
- Angiogenesis and VEGF in Cancer
- Biosensors and Analytical Detection
- Folate and B Vitamins Research
- Immune Response and Inflammation
- Mass Spectrometry Techniques and Applications
- Cancer-related Molecular Pathways
- Plant tissue culture and regeneration
Harvard University
2023
Massachusetts General Hospital
2023
Heilongjiang University
2020-2021
Harbin University
2020
Dalian Minzu University
2018
Second Military Medical University
2009-2016
Changhai Hospital
2009-2016
University of Cincinnati Medical Center
2003-2011
University of Cincinnati
1999-2007
Shanxi University
1988
MicroRNAs (miRNAs) are small noncoding RNAs that post-transcriptional regulate gene expression in a variety of cancers. Increasing evidences indicate miR-30 is down-regulated numerous human cancers including non-small cell lung cancer (NSCLC) which hypothesizes may play an important role tumorigenesis. The aim this study was to investigate the target and its roles tumor growth NSCLC. Luciferase reporter assays were employed validate regulation putative miR-30. effect on endogenous levels...
Abstract Background The enhancer of zeste homolog 2 (EZH2) was found to be overexpressed and associated with tumor metastasis in esophageal squamous cell carcinoma (ESCC). On the other hand, it reported that miR-26a, miR-98, miR-101, miR-124, miR-138 miR-214 could inhibit expression EZH2 some tumors. However, role miRNAs regulation human ESCC has not been documented. aim this study determine these via overexpression ESCC. Methods results mRNA were examined by qPCR protein detected western...
Abstract The actions of 1,25-dihydroxyvitamin D3 (1,25(OH)2 D3) are mediated through the nuclear vitamin D receptor (VDR). regulation VDR abundance plays an important role in determining magnitude target cell response to 1,25(OH)2D3. major physiological activity 1,25(OH)2D3 is calcium absorption small intestine, and level factor this regulation. However, characterization gene expression intestine remains unknown. In present study, we investigated human (hVDR) intestine. 4.0 kb 5′-flanking...
Accumulating evidence indicates that Voltage Dependent Anion Channel 1 (VDAC1) correlates with the initiation and progression of non-small cell lung cancer (NSCLC). However, regulatory mechanism VDAC1 in NSCLC remains unclear. Previous studies have reported expression miR-320a was decreased human primary squamous carcinoma, which prompted us to investigate whether there is a functional link between high VDAC1. In present report, using computational analysis, we first show has potential...
A convincing line of evidence is being developed that the congenital nongoitrous hypothyroidism and dwarfism observed in WIC-rdw rat may indeed be caused by a primary defect thyroid hormonogenesis. In support this hypothesis, several recent reports have shown presence elevated molecular chaperone levels thyrocytes, endoplasmic reticulum which was markedly dilated, suggesting intracellular protein transport. Here studies were undertaken to identify precise rat. First, genetic linkage analysis...
It has been suggested that a thyroglobulin (Tg)-R19K missense mutation may be newly identified cause of human congenital goiter, which is surprising for this seemingly conservative substitution. Here, we have examined the intracellular fate recombinant mutant Tg expressed in COS-7 cells. Incorporation R19K largely blocked secretion, and was approximately 90% degraded intracellularly over 24-h period after synthesis. Before its degradation, Tg-R19K exhibited abnormally increased association...
Annexin IV (AIV), a Ca2+-dependent membrane-binding protein, is expressed in many epithelia. modifies membrane bilayers by increasing rigidity, reducing water and H+ permeability, promoting vesicle aggregation, regulating ion conductances, all manner. We have characterized mouse which gene trap has been inserted into the first intron of annexin IV. Processing primary transcript disrupted. Northern blot immunoblot data indicated that expression was eliminated but not tissues....
A cobalt coordination polymer was prepared, which expresses multiple fluorescence responses to transition metal ions, antibiotics, pesticides and nitrobenzene.
Lung cancer is a major health problem worldwide. The aim of this study to investigate aquaporin 3 (AQP3) expression and its relationship with the clinicopathologic characteristics non-small cell lung (NSCLC).AQP3 microvascular density (MVD) tissue samples from 180 cases NSCLC were detected by immunohistochemistry.AQP3 was negative in 25 (13.9%), positive 67 (37.2%), strongly 88 (48.9%) cases, which significantly higher than that normal (P<0.01). A significant correlation found between AQP3...
A naphthalene‐containing dicarboxylic ligand, 4‐(carboxymethoxy)‐6‐methyl‐1‐(p‐tolyl)‐2‐naphthoic acid (Hcmtna), is synthesized and used to construct a coordination compound, Co(H 2 O) 4 (cmtna) ( Co‐cmtna ), through the hydrothermal strategy. The moderate rigidity flexibility of organic molecule result low number cobalt ion with ligand provide more possibilities for potential applications. Experimental results show that fluorescence can be quenched by multiple objects, including...
It is well-known that angiopoietin-2 (Ang-2) plays an important role in the formation of blood vascular system. Angiopoietin involved many diseases about angiogenesis such as tumor, so may have great prospects for treatment these diseases. The aim this study to evaluate influence inhibiting Ang-2 via adeno-associated virus induced RNA interference (RNAi) on biological characteristics bronchogenic adenocarcinoma.AAV-Ang-2shRNA driven by H1 promoter was constructed transfect A549 cell line....
Objective The aim of this study was to assess the value △Np63α in predicting tumor recurrence after curative resection esophageal squamous cell carcinoma (ESCC) patients.Methods We analyzed protein cxpression 304 clinicopathologically characterized ESCC cases by immunohistochemistry.Results found expression positive 122 (40％) cases.△Np63α higher cancer tissue than non-tumorous control at level(P =0.034).There a significant difference patients categorized according invasive depth (P...
Currently, many prostate cancer patients, detected through the specific antigen test, harbor organ-confined indolent disease that cannot be differentiated from aggressive according to clinically and pathologically known measures. Spermine has been considered as an endogenous inhibitor for prostate-confined growth its expression shown correlation with rates. If established clinically, measurements of spermine bio-synthesis rates in prostates may predict patient outcomes. Using rat models, we...
Objective To investigate whether RNA interference could induce gene silencing in lung adenocarcinoma cells as well assess the degree of vascular endothelial growth factor(VEGF) and angiopoietin2(Ang2) its effect on functional outcome. Methods Recombinant adenovirus AdVEGF shRNA AdAng2 were constructed driving by H1 promoter targeted VEGF Ang2 utilizing recombinant technology.A549 was transfected with adenovirus.30 nude rats randomly divided into 5 equal groups to be transplanted...
Lung cancer is a major worldwide health problem. The aim of this study to establish novel Chinese human lung adenocarcinoma cell line and examine its biological characteristics.Lung specimens were freshly resected during surgery. tissues incubated in vitro the was named Ch-Huang-1. characteristics cells investigated by light microscopy, chromosome analysis, transplantation experiment.Light microscopy revealed that from primary tumor, Ch-Huang-1 line, transplanted tumor possessed malignant...