A5053097964- Bone health and osteoporosis research
- Bone health and treatments
- Connexins and lens biology
- Bone Metabolism and Diseases
- Wnt/β-catenin signaling in development and cancer
- Heat shock proteins research
- Vitamin D Research Studies
- Bone and Joint Diseases
- Protein Kinase Regulation and GTPase Signaling
- Parathyroid Disorders and Treatments
- Ion channel regulation and function
- Estrogen and related hormone effects
- Marriage and Sexual Relationships
- Metal Extraction and Bioleaching
- Multiple Myeloma Research and Treatments
- Hip and Femur Fractures
- Connective tissue disorders research
- Cancer-related gene regulation
- MXene and MAX Phase Materials
- Metabolism, Diabetes, and Cancer
- Protein Degradation and Inhibitors
- Kruppel-like factors research
- Nicotinic Acetylcholine Receptors Study
- Dental Radiography and Imaging
- Erythrocyte Function and Pathophysiology
Washington University in St. Louis
2015-2024
NIHR Oxford Musculoskeletal Biomedical Research Centre
2024
Zero to Three
2020-2022
American Society for Bone and Mineral Research
2019-2022
John Wiley & Sons (United States)
2019-2022
Hudson Institute
2019-2020
Third Way
2020
BJC HealthCare
2018
Jewish Hospital
1988-2015
Missouri Bone and Joint Center
1998-2011
<h3>Background</h3> Antiresorptive agents for the treatment of osteoporosis suppress bone remodeling and reestablish turnover at a lower rate to reduce loss. Recombinant teriparatide (human parathyroid hormone 1-34) stimulates formation, increases mass, improves microarchitecture. We contrasted effects once-daily doses 20 μg 10 mg alendronate sodium on mineral density (BMD) markers turnover. <h3>Methods</h3> Markers areal BMD were assessed in 203 postmenopausal women with an 18-month...
To investigate the effectiveness of calcitonin treatment postmenopausal osteoporosis in relation to bone turnover, we examined 53 osteoporotic women before and after one year therapy with salmon (sCT), at dose 50 IU every other day. Baseline evaluation revealed that 17 (32%) patients had high turnover (HTOP), 36 (68%) normal (NTOP) as assessed by measurement whole body retention (WBR) 99mTc-methylene diphosphonate. The two groups did not differ terms mineral content (BMC) measured dual...
Connexin(Cx)43 is the major gap junction protein present in osteoblasts. We have shown that overexpression of Cx45 osteoblasts expressing endogenous Cx43 leads to decreased cell–cell communication (Koval, M., S.T. Geist, E.M. Westphale, A.E. Kemendy, R. Civitelli, E.C. Beyer, and T.H. Steinberg. 1995. J. Cell Biol. 130:987–995) transcriptional downregulation several osteoblastic differentiation markers (Lecanda, F., D.A. Towler, K. Ziambaras, S.-L. Cheng, M. Koval, Steinberg, Civitelli....
To define the potential role of subclinical vitamin D deficiency in postmenopausal bone loss, we analyzed levels circulating 25-hydroxyvitamin (25OHD) 539 midwestern caucasian women screened for osteoporosis. Low 25OHD (less than 38 nmol/L) was found 49 subjects (aged 52-77 yr). Women with low had a reduced vertebral density (VBD), assessed by quantitative computed tomography, compared to age-matched controls (P less 0.001). They also significantly lower serum calcium and phosphate, urinary...
Abstract Objective Although oral bisphosphonates are effective treatments for postmenopausal women with osteoporosis, dosing may be unsuitable some patients. An efficacious intravenously administered bisphosphonate could beneficial such Ibandronate, a potent nitrogen‐containing bisphosphonate, can using extended intervals, either orally or by rapid intravenous injection. The aim of this study was to identify the optimal regimen ibandronate in osteoporosis. Methods In randomized,...
Bone-forming cells are organized in a multicellular network interconnected by gap junctions. In these cells, junctions formed connexin43 (Cx43) and connexin45 (Cx45). Cx43 form pores that more permeable to negatively charged dyes such as Lucifer yellow calcein than Cx45 pores. We studied whether altering junctional communication manipulating the relative expression of affects osteoblast phenotype. Transfection express primarily (ROS 17/2.8 MC3T3-E1) decreased both dye transfer osteocalcin...
Estrogen treatment improves calcium malabsorption induced by surgical or natural menopause, but the mechanisms involved are still under debate, with both increased production of 1,25-dihydroxyvitamin D3 [1,25-(OH)2D3] and improved peripheral responsiveness to steroid having been proposed. To address this issue, we studied effect short term administration 1,25-(OH)2D3 (1 μg/day for 7 days) on intestinal fractional absorption 47Ca (47Ca FA) vertebral bone density, measured dual photon...
Many cells coordinate their activities by transmitting rises in intracellular calcium from cell to cell. In nonexcitable cells, there are currently two models for intercellular wave propagation, both of which involve release inositol trisphosphate (IP3)- sensitive stores. one model, IP3 traverses gap junctions and initiates the stores neighboring cells. Alternatively, waves may be mediated not junctional communication, but rather autocrine activity secreted ATP on P2 purinergic receptors. We...
Parathyroid hormone (PTH) produced a dose-dependent immediate stimulation of inositol triphosphate and diacylglycerol production in the opossum kidney cell line, primary culture proximal tubular cells, basolateral membranes from canine segments. The increase was accompanied by minor phosphate no significant bisphosphate production. Associated with changes diacylglycerol, there an hydrolysis phosphatidylinositol 4'5-bisphosphate. effect on phospholipid followed phosphorylation 4'...
We have examined cell coupling and expression of gap junction proteins in monolayer cultures cells derived from human bone marrow stromal (BMC) trabecular osteoblasts (HOB), the osteogenic sarcoma line, SaOS-2. Both HOB BMC were functionally coupled, since microinjection Lucifer yellow resulted dye transfer to neighboring cells, with averages 3.4 +/- 2.8 (n = 131) 8.1 9.3 51) coupled per injection, respectively. In contrast, little diffusion was observed SaOS-2 monolayers (1.4 1.8 n 100)....
Mutations of critical components the Wnt pathway profoundly affect skeletal development and maintenance, probably via modulation beta-catenin signaling. We tested hypothesis that is involved in mesenchymal lineage allocation to osteogenic cells using a mutant with constitutive transcriptional activity (DeltaN151). Although this stable had no effects by itself on differentiation multipotent embryonic cell lines, it synergized bone morphogenetic protein-2 (BMP-2) resulting dramatic stimulation...
A previous study reported by this group found that patients in periodontal maintenance programs taking vitamin D and calcium supplementation had a trend for better health compared to not supplementation. The objective of the present is determine, same cohort subjects, whether such differences persist over 1-year period.
<h3>Background</h3> There is an increased prevalence of osteoporosis among patients with celiac disease. However, the relative disease osteoporotic and nonosteoporotic populations not known, benefit screening population for remains controversial. <h3>Methods</h3> We evaluated 840 individuals, 266 574 without osteoporosis, from Washington University Bone Clinic by serologic Individuals positive test results antitissue transglutaminase or antiendomysial antibody were offered endoscopic...
1,25-dihydroxycholecalciferol (1,25(OH)2D3) rapidly affects calcium (Ca2+) transport in several cell systems, suggesting physiological actions independent of genomic activation. To test this hypothesis, we studied immediate to early effects (0.5–300 sec) 1,25(OH)2D3 on cytosolic ca2+ [ca2+h single osteogenic sarcoma ROS 17/2.8 cells loaded with fura-2. An acute rise [ca2+]i was observed 40% the following addition 1,25(OH)2d3, a threshold concentration 10-11 M. In most cases, transient,...
Connexin43 (Cx43) is involved in bone development, but its role adult homeostasis remains unknown. To overcome the postnatal lethality of Cx43 null mutation, we generated mice with selective osteoblast ablation Cx43, obtained using a Cx43fl allele and 2.3-kb fragment alpha1(I) collagen promoter to drive Cre osteoblasts (ColCre). Conditionally osteoblast-deleted ColCre;Cx43-/fl show no malformations at birth, develop low peak mass remain osteopenic age, exhibiting reduced formation defective...
Background: A low dietary intake of vitamin D and calcium hastens bone loss osteoporosis. Because metabolites may also alter the inflammatory response have antimicrobial effects, we studied whether use supplements affects periodontal disease status. Methods: cohort 51 subjects receiving maintenance therapy was recruited from two dental clinics; 23 were taking (≥400 IU/day) (≥1,000 mg/day) supplementation, 28 not such supplementation. All had at least interproximal sites with ≥3 mm clinical...
Oculodentodigital dysplasia (ODDD) is a dominant negatively inherited disorder with variable but characteristic anomalies of the fingers and toes, eyes, face teeth, which are caused by mutations in connexin 43 (Cx43) gene. All analyzed so far have negative influence on conductance through gap junctional channels hemichannels, as well trafficking Cx43 protein transfected cells. In this study, we inserted human Cx43G138R point mutation into mouse gene generated mice conditionally expressing...
Connexin43 (Cx43) has an important role in skeletal homeostasis, and Cx43 gene (Gja1) mutations have been linked to oculodentodigital dysplasia (ODDD), a human disorder characterized by prominent abnormalities. To determine the function of at early steps osteogenesis its ODDD phenotype, we used Dermo1 promoter drive Gja1 ablation or induce mutation chondro-osteogenic linage. Both null mutant mice develop age-related osteopenia, primarily due progressive enlargement medullary cavity cortical...