Hodgkin's disease (HD) has long been suspected to have an infectious precursor, and indirect evidence implicated Epstein-Barr virus (EBV), a ubiquitous herpesvirus, as causal agent. Recent molecular studies using EBER in situ hybridization or latency membrane protein-I (LMP-I) immunohistochemistry identified EBV latent infection up 50% of HD tumors. However, the epidemiologic features these cases not examined detail. To explore epidemiology EBV-positive so understand role etiology more...

Diffuse large B-cell lymphoma (DLBCL), the most common group of malignant lymphomas, account for 30% adult non-Hodgkin lymphomas. The 2008 World Health Organization (WHO) classification included a new entity, Epstein-Barr virus (EBV)+ DLBCL elderly, affecting patients aged 50 years or older. However, some reports younger EBV+ cases, without evidence underlying immunosuppression, can be found. role EBV in tumor microenvironment composition is still not well understood. Our aim was to assess...

Introduction Epstein-Barr virus (EBV)-associated tumors show different expression patterns of latency genes. Since in breast carcinoma this pattern is not yet fully described, our aim was to characterize EBV positive series. Methods The study conducted on 71 biopsies and 48 non-neoplastic controls. EBNA1, LMP2A LMP1 assessed by immunohistochemistry with monoclonal antibodies, while viral genomic DNA EBERs RNA transcripts performed situ hybridization. presence confirmed PCR. Results EBNA1...

Background/Aims Liver biopsy represents the gold standard for damage evaluation, but noninvasive serum markers that mirror liver fibrosis progression are actual goals both in adults and especially children. The aim was to determine specific correlate with during chronic HCV infection. Methods biopsies concomitant samples from 22 pediatric adult patients were analyzed. Histological parameters evaluated. On TGF-ß1, tissue inhibitor of matrix metalloprotein inhibitor-1 (TIMP-1), hyaluronic acid...

Non-Hodgkin's lymphoma represents 6-10% of pediatric malignancies, and diffuse large B-cell (DLBCL) is one the three major subtypes. The 2008 WHO classification included a new entity, Epstein-Barr virus (EBV)-positive DLBCL elderly, affecting patients >50 years. It has been demonstrated that EBV may play role in tumor microenvironment composition, disturbing antitumor immune response disease progression. As most studies were performed adults, our aim was to assess presence latency pattern,...

Abstract Epstein–Barr Virus (EBV) is present in neoplastic cells of 15% Asian and Latin-American diffuse large B-cell lymphoma (DLBCL) patients. Even though a tolerogenic microenvironment was recently described DLBCL, little known concerning immunomodulatory features induced by EBV. As suggested Hodgkin lymphoma, EBV-specific cytotoxic T-cells are increased but showing immune exhaustion features. Hence, host immunity suppression may play critical role tumor progression. This study aimed to...

A high cure rate may be attained for locally advanced, undifferentiated nasopharyngeal carcinoma (NPC) in children, provided that a combined modality of treatment is employed. Both local and systemic therapies are necessary. Results at single pediatric institution were analyzed.From November 1988 to December 1997, 16 consecutive patients treated with NPC the Hospital Garrahan Buenos Aires, Argentina. The authors able evaluate 11 (9 boys 2 girls); their median age was 12 (range, 8-14) years....

Abstract Epstein‐Barr virus (EBV)‐mediated B cell transformation is achieved predominantly through the action of latent proteins, but recent evidence suggests that lytic EBV replication has also a certain pathogenic role in lymphomagenesis, at least early phases transformation. Particularly, diffuse large lymphoma (DLBCL), cycle by and unexplored, so to disclose contribution our aim was evaluate viral late gene expression relation several immune response markers series EBV+ DLBCL from...

CD4 T cells play a key role in Epstein Barr virus (EBV) infection, by modulating latent antigen expression, and exhibiting cytotoxic regulatory properties. Our aim was to evaluate the presence of Granzyme B (GZMB) Foxp3 at different EBV infection status latency profiles. We examined CD4, GZMB, Foxp3, IL10, TGF-β, CD4-GZMB CD4-Foxp3 expression tonsils pediatric patients with infective CD4+, GZMB+, Foxp3+, CD4-GZMB+ CD4-Foxp3+ cell counts were higher interfollicular region. Higher found...

Abstract Context.—Because the etiology and progression of breast carcinoma remain unclear, novel mechanisms disease pathogenesis need to be considered. Recent interest has focused on Epstein-Barr virus (EBV), an oncogenic ubiquitous herpesvirus. Investigations this association could not only broaden understanding cancer but also have implications regarding early detection, treatment, prevention. Objective.—To assess EBV presence in Argentine series. Design.—Breast biopsy specimens 69 women...

Hodgkin lymphoma (HL) shows a bimodal distribution with first peak in developing countries during childhood. The causative role and prognostic significance of Epstein-Barr virus (EBV) association patients HL is controversial. Our aim was to perform comparative study EBV 2 Latin American pediatric series, correlate it patient's survival. encoded RNAs situ hybridization latent membrane protein 1 immunohistochemistry were performed on formalin-fixed, paraffin-embedded biopsies from 176 public...

Background The etiology and the molecular mechanisms related to breast carcinogenesis remain poorly understood. Some recent reports have examined role of Human Papillomavirus (HPV) in this disease. purpose study was determine prevalence HPV cancer. Methods Sixty one fresh frozen cancers samples were analyzed. Samples tested for by PCR, products automatically sequenced. Findings correlated with clinical pathological characteristics. Results DNA cancer 26% (16/61). Clinical parameters not...

Abstract The immune response is critical in NAFLD pathogenesis, but the liver infiltrate’s composition and role of each T cell population still up for debate. To characterize pathogenesis pediatric adult cases, frequency localization populations [Cytotoxic Lymphocytes (CD8+), helper (CD4+), Regulatory lymphocytes (Foxp3+) Th17 (IL-17A+)] were evaluated. In portal/periportal (P/P) tracts, both age groups displayed a similar proportion CD8+ CD4+ lymphocytes. However, comparable Foxp3+ IL-17A+...

Social distancing, hand hygiene, mask wearing, surface decontamination, travel restrictions, and school closures have been implemented worldwide to control coronavirus disease 2019 (COVID-19). It was reported that the number of EBV infections as well age characteristics infected persons before after COVID-19 pandemic significantly decreased in children from China. Since no studies explored changes EBV-associated lymphomas so far, our aim explore infection viral-associated Hodgkin lymphoma...

Extracellular vesicles (EVs) are an increasingly promising tool for liquid biopsy in liver diseases. Hepatitis C Virus (HCV) infection, alone or together with Human Immunodeficiency (HIV) infection significantly impacts on the microRNA (miRNA) EVs content resembling chronic hepatitis (CHC) progression. The objective of study was to delve into intricate EVs-miRNA profiles CHC patients different fibrosis stages, aiming pinpoint non-invasive markers capable distinguishing significant fibrosis....

Most genomic studies on Epstein–Barr virus variability have focused the geographic and pathological associations of EBV1 genomes. In contrast, EBV2 genomes has been less explored, mainly due to their restricted circulation lesser number sequenced isolates. this study, we analyzed twenty-eight ten a potential recombinant from Argentina, which were combined with two-hundred-and-thirty-nine downloaded complete other regions, produce an initial multi-sample.vcf file comprising 278 EBV context,...

Thyroid cancer is the primary endocrine malignancy, exhibiting distinct genomic drivers. The frequency of genetic alterations varies between adult and pediatric groups across geographic regions ethnicities. Molecular markers may serve as prognostic tools and/or specific treatment-selection in papillary thyroid carcinoma (PTC) children. We sought to characterize molecular PTC from Argentina test a future laboratory algorithm for diagnosis stratification. Immunohistochemistry, fluorescence...

Hodgkin's disease (HD) has long been suspected to have an infectious precursor, and indirect evidence implicated Epstein-Barr virus (EBV), a ubiquitous herpesvirus, as causal agent. Recent molecular studies using EBER in situ hybridization or latency membrane protein-1 (LMP-1) immunohisto-chemistry identified EBV latent infection up 50% of HD tumors. However, the epidemiologic features these cases not examined detail. To explore epidemiology EBV-positive so understand role etiology more...

Abstract Context.—Because the etiology and progression of breast carcinoma remain unclear, novel mechanisms disease pathogenesis need to be considered. Recent interest has focused on Epstein-Barr virus (EBV), an oncogenic ubiquitous herpesvirus. Investigations this association could not only broaden understanding cancer but also have implications regarding early detection, treatment, prevention. Objective.—To assess EBV presence in Argentine series. Design.—Breast biopsy specimens 69 women...

Abstract Apoptosis involvement in liver damage related to hepatitis C virus (HCV) chronic infection has been suggested. Although biopsy represents the gold standard for evaluating disease severity, non‐invasive tests are a growing medical need. The aim of this study was detect apoptosis markers and serum from pediatric HCV‐infected patients assess its utility predict progression. Twenty‐three were included. Liver biopsies used histological analysis as well immunodetection viral protein (NS3)...