A5025879887- Metabolomics and Mass Spectrometry Studies
- Analytical Chemistry and Chromatography
- Microfluidic and Capillary Electrophoresis Applications
- Sulfur Compounds in Biology
- Biochemical effects in animals
- Amino Acid Enzymes and Metabolism
- Diet and metabolism studies
- Alzheimer's disease research and treatments
- Innovative Microfluidic and Catalytic Techniques Innovation
- HER2/EGFR in Cancer Research
- Nanofabrication and Lithography Techniques
- Monoclonal and Polyclonal Antibodies Research
- Cancer, Hypoxia, and Metabolism
- Chromatography in Natural Products
- Tryptophan and brain disorders
- Microfluidic and Bio-sensing Technologies
- Mass Spectrometry Techniques and Applications
- Structural and Chemical Analysis of Organic and Inorganic Compounds
- Glutathione Transferases and Polymorphisms
- Pharmacological Effects and Assays
- CAR-T cell therapy research
- Folate and B Vitamins Research
- Alcoholism and Thiamine Deficiency
- Chemical Reactions and Mechanisms
- Advanced Biosensing Techniques and Applications
The University of Tokyo
2013-2024
Ajinomoto (Japan)
2020-2023
Suzuki (Japan)
2023
The site-directed chemical conjugation of antibodies remains an area great interest and active efforts within the antibody-drug conjugate (ADC) community. We previously reported a unique site modification using class immunoglobulin-G (IgG) Fc-affinity reagents to establish versatile, streamlined, site-selective native enhance therapeutic index resultant ADCs. This methodology, termed "AJICAP", successfully modified Lys248 produce site-specific ADC with wider than Food Drug...
A method for analyzing biothiols based on high-performance liquid chromatography (HPLC)-fluorescence detection under hydrophilic interaction (HILIC) conditions has been developed. Thiols were derivatized with nonfluorescent ammonium 7-fluoro-2,1,3-benzoxadiazole-4-sulfonate (SBD-F), which selectively reacts the thiol groups to furnish corresponding fluorescent SBD-thiols. Among six different kinds of HILIC columns examined, ZIC-HILIC column sulfobetaine in stationary phase proved be best...
Background: To delay the onset of dementia, it is important for healthy adults to take preventive actions before cognitive function clearly declines. Protein malnutrition a potential risk factor senile although precise link between protein/amino acid nutrition and unknown. The purpose this study was examine effect ingestion seven selected essential amino acids as granular powder, namely, leucine, phenylalanine, lysine supplemented with isoleucine, histidine, valine, tryptophan on...
Nutritional epidemiology shows that insufficient protein intake is related to senile dementia. The levels of in aged people are positively associated with memory function, and elderly high have a low risk mild cognitive impairment. Although the beneficial roles nutrition maintaining brain function well demonstrated, little known about mechanism by which dietary affects conditions. We fed mice diet (LPD) for 2 months, caused behavioral abnormalities, examined nutritional effect essential...
The site-directed chemical conjugation of antibodies remains an area great interest and active efforts within the antibody-drug conjugate (ADC) community. We previously reported a unique site modification using class immunoglobulin-G (IgG) Fc-affinity reagents to establish versatile, streamlined, site-selective native enhance therapeutic index resultant ADCs. This methodology, termed “AJICAP,” successfully modified Lys248 produce site-specific ADC with wider than Food Drug...
Intake of specific seven essential amino acids affects the pathology brain.
To realize efficient, fast separations on pillar array columns with turns, a novel turn low-dispersion and low-pressure-drop properties was developed. This "pillar-distribution-controlled" (PDC) designed as constant-radius filled octagonal pillars that were arranged to control the linear velocity of mobile phase in radial direction. After positions adjusted by computational fluid dynamics analysis, 27 mm long two turns fabricated 20 × mm2 silicon glass plate. The PDC suppressed sample...
High-performance liquid chromatography-fluorescence detection using a hydrophilic interaction chromatography-mode column (ZIC®-HILIC) was used to determine four kinds of thiol compounds in human serum. Sera were obtained from 34 subjects for this study (17 male aged 22-38 years and 17 female 18-38 years). Serum cysteine, cysteinylglycine, glutathione, γ-glutamylcysteine, derivatized with ammonium 7-fluoro-2,1,3-benzoxadiazole-4-sulfonate, separated on the ZIC®-HILIC quantified. The serum...
The chemical derivatization of target analytes can enhance the sensitivity and selectivity separation-based methods for metabolite analysis using microfluidic devices. However, development chromatography-based devices with integrated units is challenging. In this study, a novel unit pillar array (PA)-based mixing channel was developed postcolumn during on-chip liquid chromatography (LC). PA mixer enhanced between reagents in transverse direction, while preventing analyte dispersion flow...
Biological thiols – homocysteine (Hcy), cysteine (Cys), γ-glutamylcysteine (γGluCys), glutathione (GSH), and cysteinylglycine (CysGly) in plasma samples of cystathionine β-synthase (CBS, EC 4.2.1.22)-deficient mice were quantified using hydrophilic interaction liquid chromatography (HILIC) fluorescence detection. Mice deficient CBS provide a model mouse homocystinuria, an inherited metabolic disease characterized by the abnormal accumulation Hcy urine blood. For quantification, derivatized...
Hydrophilic interaction liquid chromatographic (HILIC) separation of biologically important thiols derivatized with 4-aminosulfonyl-7-fluoro-2,1,3-benzoxadiazole (ABD-F) was performed for the first time. After optimization mobile phase conditions, including acetonitrile content, pH, and buffer concentration, eight ABD-thiols (cysteamine, cysteine, N-acetylcysteine, glutathione, homocysteine, cysteinylglycine, γ-glutamylcysteine, N-(2-mercaptopropionyl)glycine as an internal standard) were...
• 2-HG underwent lactonization before forming a fluorescent derivative with NBD-PZ. A 2D liquid chromatography system an ODS column and chiral established. Enantiomers in U937 cells successfully quantified. IDH mutant had more D-2-HG than wild-type cells. In this study, heart-cutting two-dimensional (2D-LC) method for the determination of intracellular 2-hydroxyglutarate (2-HG) enantiomers is proposed. oncometabolite produced by tumors harboring isocitrate dehydrogenase (IDH) mutations....
In this study, we investigated suitable conditions for the reduction of disulfides in mouse serum samples by tris(2-carboxyethyl) phosphine (TCEP) fast analysis total biothiols. Disulfides were reduced with TCEP, and then, thiols derivatized fluorogenic reagent, ammonium 7-fluoro-2,1,3-benzoxadiazole-4-sulfonate (SBD-F). Interference peaks on chromatograms disappeared when TCEP reaction was conducted ice instead at room temperature, which is used classically. Low-molecular-weight disulfides,...
Abstract Background Nutritional epidemiology has implicated dietary protein intake in senile dementias. However, mechanistic links of and amino acids to age‐related neurodegenerative conditions remain unclear. Here, we tested the hypothesis that could affect neuronal functions survival a mouse model tauopathies, which develops tau pathologies leading progressive brain atrophy with aging. Methods Non‐transgenic (nTg) tauopathy (rTg4510) mice were fed normal diet (NPD) or low (LPD) from 3...
MEMS liquid chromatography (LC) microchip with low dispersion and a pressure drop turn structure was fabricated. In the structure, we used higher density pillar array at inner side while lower outer to control speed of sample flow. An LC chip an improved can obtain similar theoretical plate number (1233) compared previously proposed tapered (1135). The necessary inlet is also reduced from 2.7 0.4 MPa under flow rate 10 μL/min. These results show applicable for fast analysis time high conditions.