A5102815563- Pregnancy and preeclampsia studies
- Preterm Birth and Chorioamnionitis
- Neonatal Respiratory Health Research
- Reproductive System and Pregnancy
- Neonatal and Maternal Infections
- Birth, Development, and Health
- Gestational Diabetes Research and Management
- Pregnancy-related medical research
- Immune Response and Inflammation
- Blood Coagulation and Thrombosis Mechanisms
- Maternal and fetal healthcare
- Advanced Glycation End Products research
- Congenital Diaphragmatic Hernia Studies
- Amyloidosis: Diagnosis, Treatment, Outcomes
- Pregnancy and Medication Impact
- Infant Nutrition and Health
- Neonatal and fetal brain pathology
- Reconstructive Surgery and Microvascular Techniques
- Cardiovascular Issues in Pregnancy
- Neonatal Health and Biochemistry
- RNA modifications and cancer
- Connective tissue disorders research
- Cancer-related molecular mechanisms research
- Reproductive tract infections research
- Angiogenesis and VEGF in Cancer
University of Illinois Chicago
2019-2024
Illinois College
2021-2023
University of Illinois Urbana-Champaign
2021-2023
Yale University
2006-2022
Nationwide Children's Hospital
2014-2020
The Ohio State University
2014-2018
University of Edinburgh
2011-2012
The Queen's Medical Research Institute
2011
United States Department of Defense
2008
University of Kansas
2007
Preeclampsia is a pregnancy-specific disorder of unknown etiology and leading contributor to maternal perinatal morbidity mortality worldwide. Because there no cure other than delivery, preeclampsia the cause iatrogenic preterm birth. We show that shares pathophysiologic features with recognized protein misfolding disorders. These include urine congophilia (affinity for amyloidophilic dye Congo red), affinity conformational state-dependent antibodies, dysregulation prototype proteolytic...
Background Proteomic analysis of amniotic fluid shows the presence biomarkers characteristic intrauterine inflammation. We sought to validate prospectively clinical utility one such proteomic profile, Mass Restricted (MR) score. Methods and Findings enrolled 169 consecutive women with singleton pregnancies admitted preterm labor or premature rupture membranes. All had a clinically indicated amniocentesis rule out intra-amniotic infection. A fingerprint (MR score) was generated from fresh...
Cerebral organoids (COs) are rapidly accelerating the rate of translational neuroscience based on their potential to model complex features developing human brain. Several studies have examined electrophysiological and neural network COs; however, no study has comprehensively investigated developmental trajectory properties in whole-brain COs correlated these with developmentally linked morphological cellular features. Here, we profiled neuroelectrical activities over span 5 months a...
Significance Pregnancy is a unique physiological state involving biological stresses that promote protein damage (misfolding) within the maternal body. Currently, little known regarding how body copes with elevated misfolding in pregnancy. This important, because accumulation of misfolded proteins underlies many human disorders, including preeclampsia, serious complication In this study, we show pregnancy zone (PZP) efficiently inhibits aggregation proteins, amyloid beta peptide, which forms...
Abstract TLRs are pattern recognition transmembrane receptors that play key roles in innate immunity. A recently discovered soluble truncated form of TLR2 (sTLR2) acts as a decoy receptor, down-regulating the host inflammatory response to bacteria. To identify presence and functional role sTLR2 modulating intraamniotic infection, we studied 109 amniotic fluid samples women with normal pregnancy outcomes (n = 28) 39) without 42) infection. We sought demonstrate signaling vitro by using...
Objective To determine the relationship between presence of amniotic fluid (AF) biomarkers characteristic inflammation (defensins 2 and 1 calgranulins C A) fetal inflammatory status at birth. Design Prospective observational cohort. Setting Tertiary referral University hospital. Population One hundred thirty‐two consecutive mothers (gestational age, median [interquartile range]: 29.6 [24.1–33.1] weeks) who had a clinically indicated amniocentesis to rule out infection their newborns. Methods...
Placenta accreta is clinically associated with maternal uterine scar. Our objective was to investigate the biochemical contribution of scarring hyperinvasive trophoblast. We hypothesised that trophoblast over-invasion in placenta aberrant invasion-site signalling growth and angiogenic factors known be involved wound healing promotion cell invasion through epithelial mesenchymal cellular programme.Cross-sectional series.Yale-New Haven Hospital.Women histologically confirmed normal abnormal...
Proteins in the urine of women with preeclampsia (PE) bind Congo Red dye (urine congophilia). We sought to determine diagnostic performance a paper-based point-of-care test detecting congophilia for rapid triage and diagnosis PE.Prospective cohort study conducted 346 consecutive pregnant evaluated PE Labour Delivery unit at our institution. The Dot (CRD) Paper Test (index test) was performed on fresh samples. CRD results were compared an expert adjudicated each case. accuracy also serum...
Background Intra-amniotic infection and/or inflammation (IAI) are important causes of preterm birth and early-onset neonatal sepsis (EONS). A prompt accurate diagnosis EONS is critical for improved outcomes. We sought to explore the cord blood proteome identify biomarkers functional protein networks characterizing in newborns. Methodology/Principal Findings studied a prospective cohort 180 premature newborns delivered May 2004-September 2009. proteomics discovery phase employing...
Abstract Classic IL-6 signaling is conditioned by the transmembrane receptor (IL-6R) and homodimerization of gp130. During trans-signaling, binds to soluble IL-6R (sIL-6R), enabling activation cells expressing solely Soluble gp130 (sgp130) selectively inhibits trans-signaling. To characterize amniotic fluid (AF) trans-signaling molecules (IL-6, sIL-6R, sgp130) in normal gestations pregnancies complicated intra-amniotic inflammation (IAI), we studied 301 women during second trimester (n =...
Severe preeclampsia is associated with increased neutrophil activation and elevated serum soluble endoglin (sEng) Flt-1 (sFlt-1) in the maternal circulation. To dissect contribution of systemic inflammation anti-angiogenic factors preeclampsia, we investigated relationships between circulating markers severe or inflammatory state during pregnancy.Serum sEng, sFlt-1, placenta growth factor, interleukin-6 (IL-6), calprotectin, plasma α-defensins concentrations were measured by ELISA 88 women...
Though recent advancement in proteomics has provided a novel perspective on several distinct pathogenetic mechanisms leading to preterm birth (inflammation, bleeding), the etiology of most births still remains elusive. We conducted multidimensional proteomic analysis amniotic fluid identify pathways related absence inflammation or bleeding.A fingerprint was generated from fresh using surface-enhanced laser desorbtion ionization time flight (SELDI-TOF) mass spectrometry total 286 consecutive...
Alterations in circulating levels of pro- and antiangiogenic factors have been associated with adverse pregnancy outcomes. Heparin is routinely administered to pregnant women, but without clear knowledge its impact on these factors.We conducted a longitudinal study 42 women. Twenty-one women received prophylactic heparin anticoagulation, 21 healthy served as controls. Compared gestational age-matched controls, treatment was increased soluble fms-like tyrosine kinase-1 (sFlt-1) the third...