A5084947075- Immunotherapy and Immune Responses
- Connexins and lens biology
- Cancer Immunotherapy and Biomarkers
- Immune Cell Function and Interaction
- Heat shock proteins research
- Nicotinic Acetylcholine Receptors Study
- Immune cells in cancer
- Extracellular vesicles in disease
- Heme Oxygenase-1 and Carbon Monoxide
- Immune Response and Inflammation
- Autophagy in Disease and Therapy
- vaccines and immunoinformatics approaches
- Cancer, Hypoxia, and Metabolism
- Postharvest Quality and Shelf Life Management
- T-cell and B-cell Immunology
- Cholangiocarcinoma and Gallbladder Cancer Studies
- Phagocytosis and Immune Regulation
- RNA Interference and Gene Delivery
- Lipid metabolism and disorders
- Peroxisome Proliferator-Activated Receptors
- Protein Structure and Dynamics
- Pancreatic and Hepatic Oncology Research
- Computational Drug Discovery Methods
- Plant Physiology and Cultivation Studies
- CAR-T cell therapy research
Metropolitan University of Technology
2020-2025
Dirección de Investigación y Desarrollo
2025
Weatherford College
2022
Millennium Institute on Immunology and Immunotherapy
2011-2020
University of Chile
2010-2020
Inserm
2014-2015
Institut Gustave Roussy
2013-2015
Institute for Economic Research
2014
Institute of Biomedical Science
2011
University College London
2011
Abstract Clear cell renal carcinomas (RCC) frequently display inactivation of von Hippel-Lindau (VHL) gene leading to increased level hypoxia-inducible factors (HIF). In this study, we investigated the potential role HIF2α in regulating RCC susceptibility natural killer (NK) cell–mediated killing. We demonstrated that line 786-0 with mutated VHL was resistant NK-mediated lysis as compared VHL-corrected (WT7). This resistance found require stabilization. On basis global expression profiling...
Although natural killer (NK) cells play an important role in the control of melanoma, hypoxic stress tumor microenvironment may impair NK-mediated cell killing by mechanisms that are not fully understood. In this study, we investigated effect hypoxia on expression and channel activity connexin 43 (Cx43) melanoma its impact their susceptibility to NK cell-mediated lysis. Our results demonstrated increases Cx43 via hypoxia-inducible factor-1α (HIF-1α) transcriptional activity. Hypoxic...
Alterations in connexin 43 (Cx43) expression and/or gap junction (GJ)-mediated intercellular communication are implicated cancer pathogenesis. Herein, we have investigated the role of Cx43 melanoma cell proliferation and apoptosis sensitivity vitro, as well metastatic capability tumour growth vivo. Connexin levels, GJ coupling rates were analysed four different human lines. Furthermore, lung metastasis high compared with low Cx43-expressing FMS cells evaluated vivo using a xenograft model....
This study characterizes, biologically and clinically, a novel type of dendritic cells (DC) produced in the short term called tumor antigen-presenting (TAPCells). In particular, we identified factors present lysate derived from heat-shocked allogeneic melanoma (TRIMEL) that are associated with TAPCells' enhanced capability to induce CD8(+) T-cell responses vitro vaccinated patients.First, extensive phenotypic functional characterization TAPCells was performed, followed by vaccination 45...
Gap junction (GJ) mediates intercellular communication through linked hemichannels from each of two adjacent cells. Using human and mouse models, we show that connexin 43 (Cx43), the main GJ protein in immune system, was recruited to immunological synapse during T cell priming as both GJs stand-alone hemichannels. Cx43 accumulation at Ag specific time dependent, required an intact actin cytoskeleton. Fluorescence recovery after photobleaching Cx43-specific inhibitors were used prove between...
Cold storage is used to inhibit peach fruit ripening during shipment distant markets. However, this cold can negatively affect the quality of when it ripened, resulting in disorders such as wooliness, browning or leathering. In order understand individual and combined biological effects that factors have on quality, we taken a comparative EST transcript profiling approach identify genes are differentially expressed response these factors.We sequenced 50,625 Expressed Sequence Tags (ESTs)...
Abstract Gap junctions (GJs) mediate intercellular communication between adjacent cells. Previously, we showed that connexin 43 (Cx43), the main GJ protein in immune system, mediates Ag transfer human dendritic cells (DCs) and is recruited to immunological synapse during T cell priming. This crosstalk contributed activation, intracellular Ca2+ responses, cytokine release. However, role of GJs NK activation by DCs cell–mediated cytotoxicity against tumor remains unknown. In this study, found...
Phosphorus deficiency is one of the major nutrient stresses affecting plant growth. Plants respond to phosphate (Pi) through multiple strategies, including synthesis high-affinity Pi transporters. In this study, expression pattern putative wheat transporter, TaPT2, was examined in roots and leaves under Pi-deficient conditions. TaPT2 transcript levels increased Pi-starved plants. A 579 bp fragment promoter sufficient drive GUS reporter gene specifically Pi-deprived wheat. This also able...
Upon tumor antigen recognition, cytotoxic T lymphocytes (CTLs) and target cells form specialized supramolecular structures, called immunological synapses, which are required for polarized delivery of granules. In previous reports, we described the accumulation connexin 43 (Cx43)-formed gap junctions (GJs) at natural killer (NK) cell–tumor cell synapse. this report, demonstrate functional role Cx43-GJs synapse established between CTLs melanoma during cytotoxicity. Using confocal microscopy,...
Abstract Background Cold acclimation is the process by which plants adapt to low, non freezing temperatures that naturally occur during late autumn or early winter. This enables resist of Temperatures similar those associated with cold are also used fruit industry delay ripening in peaches. However, peaches subjected long periods storage may develop chilling injury symptoms (woolliness and internal breakdown). In order better understand relationship between peaches, we isolated functionally...
Gallbladder cancer (GBC), although infrequent in industrialized countries, has high incidence rates certain world regions, being a leading cause of death among elderly Chilean women. Surgery is the only effective treatment, and five-year survival rate advanced-stage patients less than 10%. Hence, exploring immunotherapy relevant, GBC immunogenicity poorly understood. This study examined relationship between host immune response patient based on presence tumor-infiltrating lymphocytes at...
Alterations in microRNA (miRNA) profiles, induced by tumor microenvironment stressors, like hypoxia, allow cancer cells to acquire immune-resistance phenotypes. Indeed, hypoxia-induced miRNAs have been implicated progression through numerous cell non-autonomous mechanisms, including the direct transfer of hypoxia-responsive miRNA from immune via extracellular vesicles. Connexin-43 (Cx43)-constituted gap junctions (GJs) also involved intercellular mobilization, other biological processes. In...
Background Immune checkpoint blocker (ICB) therapy has shown survival benefits for some patients with cancer. Nevertheless, many individuals remain refractory or acquire resistance to treatment, motivating the exploration of complementary immunotherapies. Accordingly, cancer vaccines offer an attractive alternative. Optimal delivery multiple tumor-associated antigens combined potent adjuvants seems be crucial vaccine effectiveness. Methods Here, a prototype generic melanoma vaccine, named...
Gallbladder cancer (GBC) is an aggressive and highly lethal disease with relatively low global incidence, but one that constitutes a major health problem in Asian Latin American countries, particularly Chile. The identification of new tumor-associated markers potential prognosis value required for GBC clinical practice. Using immunohistochemistry/tumor tissue microarray, we evaluated the expression 17 gastrointestinal protein (CK7, CK17, CK19, CK20, CKLMW, CKHMW, MUC1, MUC2, MUC5AC, MUC6,...
// Tamara García-Salum 1, 2 , Andrea Villablanca Franziska Matthäus 3 Andrés Tittarelli Mauricio Baeza 4 Cristián Pereda M. Alejandra Gleisner Fermín E. González 2, 5 Mercedes N. López Jörg D. Hoheisel 6 Johannes Norgauer 7 Peter J. Gebicke-Haerter 8 and Flavio Salazar-Onfray 1 Disciplinary Program of Immunology, Institute Biomedical Sciences, Faculty Medicine, Universidad de Chile, 8380453 Santiago, Chile Millennium on Immunology Immunotherapy, Biological Sciences FIAS, University...