A5028987568- RNA Research and Splicing
- RNA modifications and cancer
- Extracellular vesicles in disease
- RNA and protein synthesis mechanisms
- Amino Acid Enzymes and Metabolism
- Epigenetics and DNA Methylation
- Cell Adhesion Molecules Research
- ATP Synthase and ATPases Research
- MicroRNA in disease regulation
- Cutaneous Melanoma Detection and Management
- Bacterial Genetics and Biotechnology
- Immunotherapy and Immune Responses
- Lymphatic System and Diseases
- Plant nutrient uptake and metabolism
- Drug Transport and Resistance Mechanisms
Spanish National Cancer Research Centre
2019-2024
Centro de Investigación del Cáncer
2019-2021
Universitat de Barcelona
1992
Liquid biopsies from cancer patients have the potential to improve diagnosis and prognosis. The assessment of surrogate markers tumor progression in circulating extracellular vesicles could be a powerful non-invasive approach this setting. We characterized purified lymphatic drainage also known as exudative seroma (ES) stage III melanoma obtained after lymphadenectomy. Proteomic analysis showed that seroma-derived exosomes are enriched proteins resembling progression. In addition, we found...
Significance The transport of amino acids across the plasma membrane plays a central role in physiology. heteromeric acid transporters (HATs) neutral (LAT1/CD98hc, LAT2/CD98hc, and Asc1/CD98hc) participate variety processes such as modulation glutamatergic neurotransmission synaptic plasticity, auditory function, promotion brain development tumor growth by supporting mTORC1 activity. We identify substrate specificity determinants HATs within substrate-binding cavity nearby region that holds...
Nonsense-mediated mRNA decay (NMD) is a surveillance pathway that degrades aberrant mRNAs and also regulates the expression of wide range physiological transcripts. RUVBL1 RUVBL2 AAA-ATPases form an hetero-hexameric ring part several macromolecular complexes such as INO80, SWR1, R2TP. Interestingly, RUVBL1-RUVBL2 ATPase activity required for NMD activation by unknown mechanism. Here, we show DHX34, RNA helicase regulating initiation, directly interacts with in vitro cells. Cryo-EM reveals...
The RuvB-like protein 1/2 complex (RUVBL1/2) is a hetero-hexameric ATPase essential in several cellular processes, including chromatin remodeling and the assembly of mechanistic target rapamycin 1 (mTORC1) mTORC2. aminopyrazolone CB-6644, small molecule that allosterically inhibits RUVBL1/2, displays antitumor activity animal models. However, its binding site mechanism action are not yet understood. Here, we applied cryoelectron microscopy (cryo-EM) to determine ∼2.4 Å resolution structure...
The precise location of the rhaT gene, encoding rhamnose permease, has been established between sodA and rhaC at 3605–3607 kb Kohara's physical map, which corresponds to 88·4 min on Escherichia coli chromosomal map. dependence activity product function rhaC, operon regulatory was by measuring transport in wild-type rhaC-deficient strains. sequence sodA−rhaC interval displayed a single ORF corresponding rhaT, is transcribed counterclockwise E. chromosome. shown be preceded ribosome binding...
Abstract Nonsense-mediated mRNA decay (NMD) is a surveillance pathway that degrades aberrant mRNAs and also regulates the expression of wide range physiological transcripts. RUVBL1 RUVBL2 AAA-ATPases form an hetero-hexameric ring part several macromolecular complexes such as INO80, SWR1 R2TP. Interestingly, RUVBL1-RUVBL2 ATPase activity required for NMD activation by unknown mechanism. Here, we show DHX34, RNA helicase regulating initiation, directly interacts with in vitro cells. Cryo-EM...