A5067350319- Radiation Dose and Imaging
- Radioactivity and Radon Measurements
- Radiology practices and education
- Advanced X-ray and CT Imaging
- Cell Adhesion Molecules Research
- Radioactive contamination and transfer
- RNA Research and Splicing
- Advanced Radiotherapy Techniques
- Chemokine receptors and signaling
- RNA modifications and cancer
- Cancer, Hypoxia, and Metabolism
- Immune Cell Function and Interaction
- MicroRNA in disease regulation
- Immunotherapy and Immune Responses
- Erythrocyte Function and Pathophysiology
- T-cell and B-cell Immunology
- Ubiquitin and proteasome pathways
- Metabolism, Diabetes, and Cancer
- Angiogenesis and VEGF in Cancer
- Multiple Myeloma Research and Treatments
- Autophagy in Disease and Therapy
- CAR-T cell therapy research
- Cell Image Analysis Techniques
- Digital Radiography and Breast Imaging
- Glycosylation and Glycoproteins Research
Spanish National Cancer Research Centre
2012-2025
Centro de Investigación del Cáncer
2021-2025
Universidad Autónoma de Madrid
2002-2024
Centre for Biomedical Network Research on Rare Diseases
2015-2024
Hospital Universitario La Paz
2021-2024
Instituto de Salud Carlos III
2024
Universidad de Málaga
1999-2016
Centro Nacional de Epidemiología
2016
Consejo Superior de Investigaciones Científicas
2015
Centro de Biología Molecular Severo Ochoa
2015
Abstract CAR-T-cell therapy against MM currently shows promising results, but usually with serious toxicities. CAR-NK cells may exert less toxicity when redirected resistant myeloma cells. CARs can be designed through the use of receptors, such as NKG2D, which recognizes a wide range ligands to provide broad target specificity. Here, we test this approach by analyzing antitumor activity activated and expanded NK (NKAE) CD45RA − T from patients that were engineered express an NKG2D-based CAR....
Pharmacological activation of cannabinoid receptors elicits antitumoral responses in different cancer models. However, the biological role these tumor physio-pathology is still unknown. We analyzed CB2 receptor protein expression two series 166 and 483 breast samples operated University Hospitals Kiel, Tübingen, Freiburg between 1997 2010 mRNA previously published DNA microarray datasets. The oncogenesis was studied by generating a mouse line that expresses human V-Erb-B2 Avian...
Reversible and sub-lethal stresses to the mitochondria elicit a program of compensatory responses that ultimately improve mitochondrial function, conserved anti-aging mechanism termed mitohormesis. Here, we show harmol, member beta-carbolines family with anti-depressant properties, improves function metabolic parameters, extends healthspan. Treatment harmol induces transient depolarization, strong mitophagy response, AMPK pathway both in cultured C2C12 myotubes male mouse liver, brown...
Antigen-specific cognate interaction of T lymphocytes with antigen-presenting cells (APCs) drives major morphological and functional changes in cells, including actin rearrangements at the immune synapse (IS) formed cell-cell contact area. Here we show, using cell lines as well primary that clathrin, a protein involved endocytic processes, accumulation IS. Clathrin is recruited towards IS parallel kinetics to actin. Knockdown clathrin prevents proteins polymerization, such dynamin-2, Arp2/3...
// Michele Cioffi 1 , Sara M. Trabulo 1, 2 Mireia Vallespinos Deepak Raj Tony Bou Kheir Meng-Lay Lin Julfa Begum Ann-Marie Baker 3 Ala Amgheib Jaimy Saif 4 Manuel Perez 5 Joaquim Soriano Desco 6, 7, 8 Maria Victoria Gomez-Gaviro Lorena Cusso Diego Megias Alexandra Aicher Christopher Heeschen Stem Cells & Cancer Group, Spanish National Research Centre (CNIO), Madrid, Spain in Ageing, Barts Institute, Queen Mary University of London, UK for Tumour Biology, School Clinical Sciences,...
Abstract The possible involvement of the Rho-p160ROCK (Rho coiled-coil kinase) pathway in signaling induced by chemokine Stromal cell-derived factor (SDF)-1α has been studied human PBL. SDF-1α activation RhoA, but not that Rac. RhoA was followed p160ROCK mediated which led to myosin light chain (MLC) phosphorylation, dependent on and activities. kinetics MLC similar p160ROCK. role this cascade overall cell morphology functional responses examined employing different chemical inhibitors....
Endocytosis of chemokine receptors regulates signal transduction initiated by chemokines, but the molecular mechanisms underlying this process are not fully defined. In work, we assessed involvement motor protein nonmuscle myosin heavy chain IIA (MIIA) in endocytosis CXCR4 induced SDF-1alpha (also known as CXCL12) T lymphocytes. Overexpression C-terminal half MIIA inhibited ligand-induced CXCR4, that transferrin receptor. Targeting either silencing its expression with small interfering RNA...
The adaptive immune response depends on the interaction of T cells and antigen-presenting at synapse. Formation synapse subsequent T-cell activation are highly dependent actin cytoskeleton. In this work, we describe that express drebrin, a neuronal actin-binding protein. Drebrin colocalizes with chemokine receptor CXCR4 F-actin peripheral supramolecular cluster in interacts cytoplasmic tail both proteins redistribute to similar kinetics. knockdown impairs redistribution inhibits...
Pheochromocytomas (PCCs) and paragangliomas (PGLs) are rare neuroendocrine neoplasias of neural crest origin that can be part several inherited syndromes. Although their mRNA profiles known to depend on genetic background, a number questions related tumor biology clinical behavior remain unanswered. As microRNAs (miRNAs) key players in the modulation gene expression, comprehensive analysis could resolve some these issues. Through characterization miRNA 69 frozen tumors with germline...
Abstract Regulation of actin polymerization is critical for many different functions T lymphocytes, including cell migration. Here we show that the RhoA effector mDia induced in vitro activated PBL and highly expressed vivo diseased tissue-infiltrating lymphocytes. localizes at leading edge polarized lymphoblasts an area immediately posterior to lamella, which its protein profilin also concentrated. Overexpression mutant results inhibition both spontaneous chemokine-directed motility. does...
There is active crosstalk between tumor cells and the microenvironment during metastatic progression, a process that significantly affected by obesity, particularly in breast cancer. Here we analyze impact of high fat diet (HFD) on metastasis, focusing role platelets formation premetastatic niches (PMNs). We find HFD provokes pre-activation endothelial cells, promoting PMNs lung. These are characterized increased vascular leakiness, platelet activation overexpression fibronectin both cells....
The nucleolus is a membraneless organelle and an excellent stress sensor. Any changes in its architecture or composition lead to nucleolar stress, resulting cell cycle arrest interruption of ribosomal activity, critical factors aging cancer. In this study, we identified described the pivotal role RNA-binding protein (RBP) HNRNPK ribosome dynamics. We developed vitro model endogenous overexpression vivo mouse ubiquitous overexpression. These models showed disruptions translation caused...
The objective of this work was to estimate patient doses (dose-area product, organ dose, effective dose and entrance surface dose) for barium procedures. A total 175 procedures, in patients, five different examination categories analysed. Dose-area product determined using a transmission ionization chamber. Organ were assessed knowledge the software. For all contribution fluoroscopy greater than that from radiography. double contrast enema, enteroclysis intestinal tract procedures higher...
To calculate the difference in patient radiation dose radiologically guided interventional vascular procedures between conventional and digital systems to estimate effective energy imparted with system.A total of 318 (in patients) 15 different examination groups were analyzed. The dose-area product was determined by using a transmission chamber fitted an x-ray-tube light-beam diaphragm; software.Urinary biliary tract showed small differences average systems. products higher than system. 0.88...
One of the main problems we face with PPGL is lack molecular markers capable predicting development metastases in patients. Telomere-related genes, such as TERT and ATRX, have been recently described PPGL, supporting association between activation immortalization mechanisms disease progression. However, contribution other genes involving telomere preservation machinery has not previously investigated. In this work, aimed to analyze prognostic value a comprehensive set involved maintenance....
Congenital microcephaly (MCPH) is a neurodevelopmental disease associated with mutations in genes encoding proteins involved centrosomal and chromosomal dynamics during mitosis. Detailed MCPH pathogenesis at the cellular level still elusive, given diversity of lack comparative vivo studies. By generating series CRISPR/Cas9-mediated genetic KOs, we report here that - whereas defects spindle pole (ASPM, MCPH5) result mild development centrosome (CDK5RAP2, MCPH3) or centriole (CEP135, MCPH8)...
The multiple myeloma (MM) landscape has changed in the last few years, but most patients eventually relapse because current treatment modalities do not target clonogenic stem cells, which are drug-resistant and can self-renew. We hypothesized that side population (SP) cells represent and, searching for new strategies, analyzed anti-myeloma activity of natural killer (NK) against cells. Activated expanded NK (NKAE) products were obtained by co-culturing from MM with K562-mb15-41BBL cell line...