A5001890987- Microtubule and mitosis dynamics
- Pluripotent Stem Cells Research
- CRISPR and Genetic Engineering
- Cancer-related Molecular Pathways
- Pancreatic function and diabetes
- PI3K/AKT/mTOR signaling in cancer
- Proteoglycans and glycosaminoglycans research
- Sleep and Wakefulness Research
- Chromosomal and Genetic Variations
- Colorectal Cancer Surgical Treatments
- Immune cells in cancer
- Genetic and Kidney Cyst Diseases
- Cannabis and Cannabinoid Research
- Vascular Malformations and Hemangiomas
- Ubiquitin and proteasome pathways
- DNA Repair Mechanisms
- Plant Molecular Biology Research
- Chemokine receptors and signaling
- Congenital heart defects research
- Wnt/β-catenin signaling in development and cancer
- 3D Printing in Biomedical Research
- Fetal and Pediatric Neurological Disorders
- Photosynthetic Processes and Mechanisms
- Protist diversity and phylogeny
- Colorectal Cancer Screening and Detection
Spanish National Cancer Research Centre
2019-2024
Hospital de Especialidades
2022
Mexican Social Security Institute
2022
Institute for Social Security and Services for State Workers
2022
Centro Medico Nacional Siglo XXI
2022
Revista Conamed
2022
Universidad Complutense de Madrid
2016-2020
Instituto de Investigación Sanitaria del Hospital Clínico San Carlos
2016-2020
Autophagy is considered primarily a cell survival process, although it can also lead to death. However, the factors that dictate shift between these 2 opposite outcomes remain largely unknown. In this work, we used Δ9-tetrahydrocannabinol (THC, main active component of marijuana, compound triggers autophagy-mediated cancer death) and nutrient deprivation (an autophagic stimulus cytoprotective autophagy) investigate precise molecular mechanisms responsible for activation cytotoxic autophagy...
Glioblastoma (GBM) is one of the most aggressive forms cancer. It has been proposed that presence within these tumors a population cells with stem-like features termed Glioma Initiating Cells (GICs) responsible for relapses take place in patients this disease. Targeting cell therefore an issue great therapeutic interest neuro-oncology. We had previously found neurotrophic factor MIDKINE (MDK) promotes resistance to glioma death. The main objective work investigating role MDK regulation GICs....
Article21 February 2021Open Access Source DataTransparent process PLK1 regulates centrosome migration and spindle dynamics in male mouse meiosis Enrique Alfaro orcid.org/0000-0002-4681-147X Departamento de Biología, Facultad Ciencias, Unidad Biología Celular, Universidad Autónoma Madrid, Spain Search for more papers by this author Pablo López-Jiménez orcid.org/0000-0002-6673-5996 José González-Martínez orcid.org/0000-0002-3850-6803 Cell Division Cancer Group, Spanish National Research Centre...
Congenital microcephaly (MCPH) is a neurodevelopmental disease associated with mutations in genes encoding proteins involved centrosomal and chromosomal dynamics during mitosis. Detailed MCPH pathogenesis at the cellular level still elusive, given diversity of lack comparative vivo studies. By generating series CRISPR/Cas9-mediated genetic KOs, we report here that - whereas defects spindle pole (ASPM, MCPH5) result mild development centrosome (CDK5RAP2, MCPH3) or centriole (CEP135, MCPH8)...
Chromosome segregation requires that centromeres properly attach to spindle microtubules. This essential step regulates the accuracy of cell division and must therefore be precisely regulated. One main centromeric regulatory signaling pathways is haspin-H3T3ph-chromosomal passenger complex (CPC) cascade, which responsible for recruitment CPC centromeres. During mitosis, haspin kinase phosphorylates histone H3 at threonine 3 (H3T3ph), an epigenetic mark recruits CPC, in catalytic component...
The human cerebellum has undergone significant evolutionary expansion compared to other species, contributing both motor and cognitive skills. However, the mechanisms underlying this process remain largely unknown as interrogating cerebellar development alongside species date been unfeasible. To address this, we developed a minimally guided organoid protocol that combines unguided neural induction with precise temporal calibration of posteriorizing morphogens. This approach effectively...
Abstract Aurora A is a serine/threonine kinase whose role in cell cycle progression and tumour generation has been widely studied. Recent work revealed an unexpected function for during CD4 + T activation and, also, graft versus host disease development. However, it remains unknown whether involved CD8 effector cytotoxic lymphocyte-mediated antiviral response. Here, we show that chemical inhibition leads to impairment of both the peptide-specific cytotoxicity degranulation activity cells....
Abstract Commonly expressed at developmental transitions, microRNAs operate as fine tuners of gene expression to facilitate cell fate acquisition and lineage segregation. Nevertheless, how they might regulate the earliest transitions in early mammalian embryogenesis remains obscure. Here, a strictly vivo approach based on novel genetically-engineered mouse models single-cell RNA sequencing, we identify miR-203 critical regulator timing restriction within totipotency pluripotency transition...
The aim of this study was to compare a standard versus segmental withdrawal during screening colonoscopy and its effect on the adenoma detection rate (ADR).We performed single-center clinical trial average-risk patients 50 years age older undergoing colonoscopy. Patients were randomized into four groups: at least 6 or 8 minutes withdrawal, in which ≥3 ≥4 dedicated right side colon, with minimum time minutes, respectively.There 311 study. There no difference ADR between groups (relative ratio...
SUMMARY Brain development requires correct tissue patterning and production of appropriate cell types. Transcription factors (TFs) play essential roles in these processes, regulating the expression target genes responsible for neuronal subtypes specific features. Cell adhesion molecules are key components identities that control sorting, migration, neurite outgrowth/guidance synaptogenesis. To date, link between TFs is considered to be unidirectional. Here, we demonstrate ectopic Dbx1 leads...
Abstract Autosomal Recessive Primary Microcephaly (MCPH) is a rare disease associated to proteins involved in centrosomal and spindle dynamics including Cep135 (MCPH8). Although has been centriolar assembly, the mechanisms pathogenesis underlying MCPH8 mutations are unclear. By using series of CRISPR/Cas9-edited murine alleles, we report here that lack results perinatal lethality accompanied by significant microcephaly dosis-dependent manner. deficiency, but not other such as Aspm or...
Abstract The AKT-mTOR pathway is a central regulator of cell growth and metabolism. Upon sustained mTOR activity, AKT activity attenuated by feedback loop that restrains upstream signaling. However, how cells control the signals limit not fully understood. Here we show MASTL/Greatwall, cell-cycle kinase supports mitosis phosphorylating PP2A/B55 inhibitors ENSA/ARPP19, inhibits PI3K-AKT sustaining mTORC1- S6K1-dependent phosphorylation IRS1 GRB10. Genetic depletion MASTL results in an...
RESUMENLa gigantomastia es una patología que tratamos frecuentemente, por lo importante conocer los distintos problemas a nos podemos enfrentar.Reportamos un caso de donde el acceso mamoplastia reducción con técnica pedículo superior se modificó, debido hallazgos transoperatorios, mastectomía total injerto libre pezón.Se realizó la resección 4.8 kg en mama derecha y 3.4 izquierda.El estudio histopatológico definitivo inmunohistoquímica reportó hiperplasia estromal pseudoangiomatosa, entidad...