A5064716609- Platelet Disorders and Treatments
- Extracellular vesicles in disease
- Preterm Birth and Chorioamnionitis
- Ectopic Pregnancy Diagnosis and Management
- Pregnancy and preeclampsia studies
- Cell Adhesion Molecules Research
- Blood transfusion and management
- interferon and immune responses
- Blood groups and transfusion
- Complement system in diseases
- Assisted Reproductive Technology and Twin Pregnancy
- Systemic Lupus Erythematosus Research
- Monoclonal and Polyclonal Antibodies Research
- Pregnancy-related medical research
- T-cell and B-cell Immunology
- Transplantation: Methods and Outcomes
- Inflammasome and immune disorders
- Phagocytosis and Immune Regulation
- Gestational Diabetes Research and Management
- Prenatal Screening and Diagnostics
- Parasites and Host Interactions
- Maternal and Perinatal Health Interventions
- Alcohol Consumption and Health Effects
- Liver Diseases and Immunity
- Bacterial Infections and Vaccines
Lund University
2021-2025
Université Laval
2014-2024
Centre hospitalier universitaire de Québec
2015-2022
Wilfrid Laurier University
2020
Rabin Medical Center
2020
Héma-Québec
2016
Significance Immune complexes (ICs) form when antibodies encounter their antigens. ICs are present in blood multiple pathological conditions. Given the abundance of platelets and that they express a receptor for ICs, called Fcγ IIA (FcγRIIA), we examined impact mouse model. We found circulating induced systemic shock, characterized by loss consciousness, activating platelet FcγRIIA. Shock was mediated liberation serotonin, molecule better known its role brain, from granules. During were...
Abstract Extracellular vesicles (EV) are small membrane produced by cells upon activation and apoptosis. EVs heterogeneous according to their origin, mode of release, composition, organelle biochemical content, other factors. Whereas it is apparent that implicated in intercellular communication, they can also be used as biomarkers. Continuous improvements pre-analytical parameters flow cytometry permit more efficient assessment EVs; however, methods objectively distinguish from background,...
BACKGROUND Whereas platelet transfusion is a common medical procedure, inflammation still occurs in fraction of transfused individuals despite the absence any apparent infectious agents. Platelets can shed membrane vesicles, called extracellular vesicles (EVs), some which contain mitochondria (mito+EV). With its content damage‐associated molecular pattern (DAMP), mitochondrion stimulate innate immune system. Mitochondrial DNA (mtDNA) recognized DAMP detected milieu numerous inflammatory...
On activation, platelets release vesicles called microparticles (MPs). MPs are heterogeneous with regard to the presence or absence of mitochondria. We quantified in platelet concentrates (PCs) taking their mitochondrial content into account. Platelet-rich plasma (PRP), buffy coat (BC) and apheresis (AP) PCs were tested through 7 days storage. A combination flow cytometry spanning-tree progression analysis density-normalized events (SPADE) was used determine MP during All PC biochemical...
Abstract Mitochondria are organelles that govern energy supply and control cell death. also express bacterial features, such as the presence of inner membrane cardiolipin a circular genome rich in hypomethylated CpG motifs. While mitochondrial extrusion by damaged organs or activated cells is thought to trigger innate immunity, it unclear whether extracellular mitochondria stimulate an adaptive immune response. We describe development novel assays detect autoantibodies specific two distinct...
The mitochondrion supplies energy to the cell and regulates apoptosis. Unlike other mammalian organelles, mitochondria are formed by binary fission cannot be directly produced cell. They contain numerous copies of a compact circular genome that encodes RNA molecules proteins involved in mitochondrial oxidative phosphorylation. Whereas DNA (mtDNA) activates innate immune system if present cytosol or extracellular milieu, it is also target circulating autoantibodies systemic lupus...
Abstract Apoptotic exosome-like vesicles (ApoExos) are a novel type of extracellular vesicle that contribute to the propagation inflammation at sites vascular injury when released by dying cells. ApoExos characterized presence C-terminal perlecan LG3 fragment and 20S proteasome, they produced downstream caspase-3 activation. In present study, we assessed relative roles autophagy caspase-3-mediated pathways in controlling biogenesis secretion immunogenic ApoExos. Using electron microscopy...
Allergic asthma is characterized by type 2 inflammation and eosinophilia. Tartrate-resistant acid phosphatase 5 (TRAP5/ACP5) a metallophosphatase expressed alveolar macrophages that dephosphorylates osteopontin (OPN), phosphoglycoprotein with increased expression in asthma. To investigate the role of TRAP5 during asthma, we used murine model ovalbumin (OVA) induced allergic airway as well IL-33 including Trap5-/- wild-type (WT) mice. Histological analyses lung revealed OVA-induced formation...
CpG oligonucleotides (ODNs) are synthetic single-stranded DNA sequences that act as immunostimulants. They have been increasingly used to treat several cancers; however, thrombocytopenia is a potential recognized side effect of some sequences.
Objective The study aimed to estimate the predictive value of midtrimester cervical length (CL) and optimal cut-off CL that should be applied with asymptomatic nulliparous women for prediction spontaneous preterm birth (sPTB). Study Design This is a prospective cohort singleton gestation. Participants underwent measurement by transvaginal ultrasound between 20 24 weeks participants their health care providers remained blinded results measurement. primary outcomes were sPTB before 35 37...
Extracellular vesicles (EVs) are derived from the membrane of platelets and released into circulation upon activation or injury. Analogous to parent cell, platelet-derived EVs play an important role in hemostasis immune responses by transfer bioactive cargo cells. Platelet release increase several pathological inflammatory diseases, such as sepsis. We have previously reported that M1 protein bacterial pathogen Streptococcus pyogenes directly mediates platelet activation. In this study, were...
Mitochondria are organelles in charge of energy supply and the control apoptosis. Owing to their similarities with bacteria, however, extracellular mitochondria considered damage‐associated molecular patterns ( DAMP s) capable activating immune system non‐immune cells. Studies revealed that diverse blood products contain mitochondria, which could account for adverse reactions can occur transfusion. In this review, we discuss how mitochondrial s trigger inflammatory responses, highlight...
Platelets are active participants in maintaining haemostasis. Upon activation, platelet shed vesicles from their cytoplasmic membrane known as microparticles. These microparticles and unique cargo thought to participate intercellular communication. Platelet transfusion is frequently required for patients with low counts or disorders restore normal functions. Unfortunately, adverse reactions most commonly associated than any other type of labile blood products. In this review, we raise the...
Objective This study was aimed to estimate the value of transabdominal (TA) ultrasound measurement cervical length (CL), as an alternative transvaginal (TV) ultrasound, for universal screening short cervix in midtrimester. Study Design We conducted a prospective cohort nulliparous women with singleton pregnancy at 20 24 weeks gestation. All participants underwent TA followed by TV acquisitions images and videos uterine cervix. A second sonographer, blinded participants' data outcomes,...
<h3>Background</h3> There is a growing appreciation for the contribution of platelets to immunity; however, our knowledge mostly relies on platelet functions associated with vascular injury and prevention bleeding. Circulating immune complexes (ICs) contribute both acute chronic inflammation in multitude clinical conditions through their interaction members Fcγ receptor (FcγR) family. FcγRIIA most abundantly expressed FcγR human blood circulation. However, mice do not express FcγRIIA, murine...