A5063346822- Animal Genetics and Reproduction
- CRISPR and Genetic Engineering
- Virus-based gene therapy research
- Xenotransplantation and immune response
- Sperm and Testicular Function
- Animal Nutrition and Physiology
- Reproductive Biology and Fertility
- Gut microbiota and health
- Cancer Cells and Metastasis
- Probiotics and Fermented Foods
- Cancer Research and Treatments
- Pluripotent Stem Cells Research
- Viral Infectious Diseases and Gene Expression in Insects
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Digestive system and related health
- Antimicrobial Peptides and Activities
- 3D Printing in Biomedical Research
- Viral gastroenteritis research and epidemiology
- Tissue Engineering and Regenerative Medicine
- Moringa oleifera research and applications
- Redox biology and oxidative stress
- Renal and related cancers
- Ovarian function and disorders
- Trauma, Hemostasis, Coagulopathy, Resuscitation
- Garlic and Onion Studies
Rural Development Administration
2014-2025
National Institute of Animal Science
2016-2025
Seoul National University
2024-2025
Kyungpook National University
2021
Renji Hospital
2013
Shanghai Hospital Development Center
1986
Could the protamine-1 to protamine-2 mRNA ratio serve as a biomarker estimate fertilizing capacity of sperm from men taking part in an IVF/ICSI programme? The protamine clearly discriminates between fertile and subfertile with normal exhibit higher IVF/ICSI. Aberrant ratios are associated male factor infertility is comparable protein (due transcriptional stop elongating spermatids). study population was drawn men, whose female partners participated IVF or ICSI programmes September 2010...
Intestinal organoids offer great promise for disease-modelling-based host–pathogen interactions and nutritional research feed efficiency measurement in livestock regenerative medicine therapeutic purposes. However, very limited studies are available on the functional characterisation three-dimensional (3D) expansion of adult stem cells species compared to other species. crypts derived from intestinal under a 3D culture system small intestine bovine were successfully established characterised...
Transgenic (TG) animals offer significant potential for diverse applications but may pose risks if the impact of transgene expression on health and physiological parameters is not thoroughly assessed. This study aims to evaluate effects 3D8 scFv gene male TG chickens, focusing key biological markers, mortality, growth. Serum samples were collected at 14 weeks age from non-TG chickens comprehensive analysis serum biochemistry, sex hormones, cytokine profiles. Mortality growth monitored over a...
Establishing chicken primordial germ cells (PGCs) in vitro is critical for producing genetically modified (GM) chickens. Efficient and reliable isolation cultivation of PGCs remain significant challenges advancing avian genetic modifications. To address these challenges, we employed a streamlined practical approach the efficient stable gonadal PGCs. Chicken were isolated from embryonic gonads, surgically removed dissociated using trypsin. The by exploiting differential adhesion properties,...
Bovine viral diarrhea (BVD) is a single-stranded, positive-sense ribonucleic acid (RNA) virus belonging to the genus Pestivirus of Flaviviridae family. BVD frequently causes economic losses farmers. Among bovine (BVDV) strains, BVDV-1b predominant and widespread in Hanwoo calves. Reverse-transcription polymerase chain reaction (RT-PCR) an essential method for diagnosing has become gold standard diagnosis Republic Korea. However, this diagnostic time-consuming requires expensive equipment....
One of the reasons to causing blood coagulation in tissue xenografted organs was known incompatibility and anti-coagulation regulatory system between TG pigs primates. Thus, overexpression human CD73 (hCD73) pig endothelial cells is considered as a method reduce coagulopathy after pig-to-non-human-primate xenotransplantation. This study performed produce breed transgenic expressing hCD73 for studies immune rejection responses could provide successful application The were constructed an...
Probiotics have been defined as live microorganisms that are administered in an appropriate amount to provide health benefits the host animal. In this study, we investigated effect of L. salivarius DJ-sa-01 secreting 3D8 single-chain variable fragment (3D8 scFv) on growth performance, cytokine secretion, and intestinal microbial flora chickens. The experiment was divided into control group expressing scFv experimental group. Chicken fed 109 colony-forming units (CFUs) wild-type (WT) or...
Unlike mouse results, cloning efficiency of nuclear transfer from porcine induced pluripotent stem cells (piPSCs) is very low. The present study was performed to investigate the effect cell cycle inhibitors on synchronization piPSCs. piPSCs were generated using combination six human transcriptional factors under culture condition. To examine synchronization, cultured a matrigel coated plate with media and they treated staurosporine (STA, 20 nM), daidzein (DAI, 100 μM), roscovitine (ROSC, 10...
Recently, a scaffold-based three-dimensional (3D) culture system has provided reliable alternative platform for the establishment of intestinal organoids in vitro.
Recently, we reported the robust in vitro three-dimensional (3D) expansion of intestinal organoids derived from adult bovine (> 24 months) samples. The present study aimed to establish an 3D system for cultivation growing cattle (12 months old) practical use as a potential alternative vivo systems various purposes. However, very few studies on functional characterization and stem cells livestock species compared those other are available. In this study, crypts, including cells, small...
The present study was conducted to investigate the efficacy of oral administration L. reuteri on growth performance, intestine histomorphology, immunological and gut microbiome broilers. A total twenty healthy chickens were used in a five-week experimental trial. Birds assigned into one two groups with orally administrated probiotic without probiotic- (Control -Phosphate-buffered saline). significant (p<0.05) body weight gain observed treatment group compare those control at end In addition,...
Avian sex determination system involves the male ZZ and female ZW chromosomes. However, very few studies are reported expression, functional role importance of genes on W chromosome because its small highly heterochromatic genomic regions. Recent demonstrated that may have critical roles in physiology, subsequent sexual differentiation chickens. Therefore, gene annotation, including describing expression function chicken chromosome, is needed. In this study, we searched chickens selected a...
Abstract Background The 3D8 single chain variable fragment (scFv) is a mini-antibody sequence that exhibits independent nuclease activity against all types of nucleic acids. In this research, crossing scFv G1 transgenic rooster with wild-type hens produced G 2 chickens to evaluate suppression viral transmission. Result were identified using genomic PCR and immunohistochemistry. To Newcastle disease virus (NDV) protection conferred by expression, transgenic, non-transgenic, specific...
Abstract Background Intestinal organoids offer great promise for disease modelling based host-pathogen interactions and nutritional research feed efficiency measurement in livestock as well regenerative medicine therapeutic purposes. However, very limited studies are available on the functional characterization three-dimensional (3D) expansion of adult stem cells species compared to mammals. Therefore, we characterized intestinal derived from small intestine bovine cultivated under vitro 3D...
NANOG, as a transcription factor, plays key role in maintaining pluripotency higher vertebrates. Thus, NANOG gene expression is critical index for the transition from somatic cells to pluripotent stage. Here, we established chicken knock-in DF1 which red fluorescent protein (RFP) was specifically inserted into transcriptional start site of through CRISPR‒Cas9 (clustered regularly interspaced short palindromic repeat-CRISPR associated 9) technical platform. Subsequently, 4 factors (Pou5f3,...
In-Sul Hwang, Dae-Jin Kwon, Tae-Uk Kwak, Joo-Young Lee, Nam-Woong Hyung, Hyeon Yang, Keon Bong Oh, Sun-A Ock, Eung-Woo Park, Gi-Sun Im, and Seongsoo Hwang. Journal of Embryo Transfer 2016;31:117-21. https://doi.org/10.12750/JET.2016.31.2.117