A5044718521- SARS-CoV-2 and COVID-19 Research
- COVID-19 Clinical Research Studies
- SARS-CoV-2 detection and testing
- Animal Virus Infections Studies
- COVID-19 epidemiological studies
- Long-Term Effects of COVID-19
- Viral gastroenteritis research and epidemiology
- vaccines and immunoinformatics approaches
- Biosensors and Analytical Detection
- Influenza Virus Research Studies
- Virus-based gene therapy research
- COVID-19 and Mental Health
- Vaccine Coverage and Hesitancy
- interferon and immune responses
- Kawasaki Disease and Coronary Complications
- Immunodeficiency and Autoimmune Disorders
- Blood groups and transfusion
- Heparin-Induced Thrombocytopenia and Thrombosis
- Viral Infections and Outbreaks Research
- Infection Control and Ventilation
- Multiple Sclerosis Research Studies
- Immunotherapy and Immune Responses
- Viral Infections and Immunology Research
University of Regensburg
2021-2025
Institute of Medical Microbiology and Hygiene
2021-2025
University Hospital Regensburg
2020
It is not clear whether there an association between adverse reactions and immune response after vaccination. Seven hundred thirty-five vaccinees from our University Medical Center vaccination clinic provided information about sex, age first second with BNT162b2. Adverse were categorized into three groups: no or minor on the injection side, moderate (not further classified) severe-defined as any symptom(s) resulting in sick leave. We chose 38 most severe compared their humoral...
Vaccines in use against SARS-CoV-2 induce immune responses the spike protein. There is intense interest whether antibody response induced by vaccines will be robust new variants, as well next-generation for previously infected or immunized individuals.
The threat of spillovers coronaviruses associated with the severe acute respiratory syndrome (SARS) from animals to humans necessitates vaccines that offer broader protection sarbecoviruses. By leveraging a viral-genome-informed computational method for selecting immune-optimized and structurally engineered antigens, here we show single antigen based on receptor binding domain spike protein sarbecoviruses elicits broad humoral responses against SARS-CoV-1, SARS-CoV-2, WIV16 RaTG13 in mice,...
SARS-CoV-2 infection fatality ratios (IFR) remain controversially discussed with implications for political measures. The German county of Tirschenreuth suffered a severe outbreak in spring 2020, particularly high case ratio (CFR). To estimate seroprevalence, underreported infections, and IFR the population aged ≥14 years June/July we conducted population-based study including home visits elderly, analyzed 4203 participants antibodies via three antibody tests. Latent class analysis yielded...
The accelerated development of the first generation COVID-19 vaccines has saved millions lives, and potentially more from long-term sequelae SARS-CoV-2 infection. most successful vaccine candidates have used full-length spike protein as an immunogen. As expected RNA viruses, new variants evolved quickly replaced original wild-type SARS-CoV-2, leading to escape natural infection or induced immunity provided by sequence. Next that confer specific targeted broadly neutralising epitopes on...
The rise of SARS-CoV-2 variants has made the pursuit to define correlates protection more troublesome, despite availability World Health Organisation (WHO) International Standard for anti-SARS-CoV-2 Immunoglobulin sera, a key reagent used standardise laboratory findings into an international unitage. Using pseudotyped virus, we examine capacity convalescent from well-defined cohort healthcare workers (HCW) and Patients infected during first wave national critical care centre in UK neutralise...
<title>Abstract</title> A panzootic of H5Nx avian influenza viruses has severely affected poultry and wild bird populations, resulting in multiple mammalian spillovers, including mild to fatal human infections caused by antigenically distinct diverse virus clades. The unpredictable nature spillover events drifted hinders our ability effectively respond the heightened risk human-to-human transmission. Stockpiled strain-specific H5 whole virus-based vaccines provide limited breadth reduced...
<title>Abstract</title> A panzootic of H5Nx avian influenza viruses has severely affected poultry and wild bird populations resulting in multiple mammalian spillovers, including human infections caused by antigenically distinct diverse virus clades. The unpredictable nature spillover events drifted and/or reassorted hinders our ability to effectively respond retroactively the heightened risk human-to-human transmission. Stockpiled strain-specific H5 whole virus-based vaccines provide limited...
The novel coronavirus SARS-CoV-2 is the seventh identified human coronavirus. Understanding extent of pre-existing immunity induced by seropositivity to endemic seasonal coronaviruses and impact cross-reactivity on COVID-19 disease progression remains a key research question in immunopathology COVID-2019 disease. This paper describes panel lentiviral pseudotypes bearing spike (S) proteins for each seven (HCoVs), generated under similar conditions optimized high titre production allowing...
As of June, 2021, the UK population is only partly vaccinated against COVID-19, with many people having received just one vaccination dose (either BNT162b2 [Pfizer–BioNTech]) or ChAdOx1 nCoV-19 [AZD1222; Oxford–AstraZeneca]). Tracking spread SARS-CoV-2 Variants Concern (VOCs) remains important for understanding levels vaccine-induced immunity and identifying emergence vaccine escape variants. The immune correlates protection to COVID-19 established in phase 3 clinical trials following two...
Abstract Background Serum neurofilament light chain (sNfL) is an established biomarker of neuro-axonal damage in multiple neurological disorders. Raised sNfL levels have been reported adults infected with pandemic coronavirus disease 2019 (COVID-19). Levels children COVID-19 not as yet reported. Objective To evaluate whether elevated contracting COVID-19. Methods Between May 22 and July 22, 2020, a network outpatient pediatricians Bavaria, Germany, the Coronavirus antibody screening from...
To assess vaccine immunogenicity in non-infected and previously infected individuals a real-world scenario, SARS-CoV-2 antibody responses were determined during follow-up 2 (April 2021) of the population-based Tirschenreuth COVID-19 cohort study comprising 3378 inhabitants county aged 14 years or older. Seronegative participants vaccinated once with Vaxzevria, Comirnaty, Spikevax had median neutralizing titers ranging from ID50 = 25 to 75. Individuals two immunizations Comirnaty higher ID50s...
Abstract The emergence of severe acute respiratory syndrome-related coronavirus 2 (SARS-CoV-2) in 2019 caused an increased interest neutralizing antibody tests to determine the immune status population. Standard live-virus-based neutralization assays such as plaque-reduction or pseudovirus cannot be adapted point-of-care (POC). Accordingly, quantifying competitive binding inhibition angiotensin-converting enzyme (ACE2) receptor receptor-binding domain (RBD) SARS-CoV-2 by antibodies have been...
Antibody testing for determining the SARS-CoV-2 serostatus was rapidly introduced in early 2020 and since then has been gaining special emphasis regarding correlates of protection. With limited access to representative samples with known infection status during initial period test development validation, spectrum bias be considered when moving from a "test establishment setting" population-based settings, which antibody is currently implemented. To provide insights into presence magnitude...
Abstract SARS-CoV-2 infection fatality ratios (IFR) remain controversially discussed with implications for political measures, but the number of registered infections depends on testing strategies and deduced case (CFR) are poor proxies IFR. The German county Tirschenreuth suffered a severe outbreak in spring 2020 particularly high CFR. To estimate seroprevalence, dark figure, IFR population aged ≥14 years June/July misclassification error control, we conducted population-based study,...
Background: Children and youth are affected rather mildly in the acute phase of COVID-19 thus, SARS-CoV-2 infection may easily be overlooked. In light current discussions on vaccinations children it seems necessary to better identify who immune against due a previous understand related reactions children. Methods: cross-sectional design, aged 1-17 were recruited through primary care pediatricians for study (a) randomly, if they had an appointment regular health check-up or (b) parents...
Abstract Since cell‐based virus neutralization assays are still the gold standard to assess a patient's immune protection against given virus, they of utmost importance for serodiagnosis, convalescent plasma therapy, and vaccine development. Monitoring emergence characteristics neutralizing antibodies in an outbreak situation, confirming as correlates from infection testing vaccine‐induced potency antibody responses, quests automated, fast, parallel assays. We developed impedance‐based...
Abstract Updates of SARS-CoV-2 vaccines are required to generate immunity in the population against constantly evolving variants concerns (VOCs). Here we describe three novel in-silico designed spike-based antigens capable inducing neutralising antibodies across a spectrum VOCs. Three sets utilising pre-Delta (T2_32), and post-Gamma sequence data (T2_35 T2_36) were designed. T2_32 elicited superior responses VOCs compared Wuhan-1 spike antigen DNA prime-boost immunisation regime guinea pigs....
Herein, we provide results from a prospective population-based longitudinal follow-up (FU) SARS-CoV-2 serosurveillance study in Tirschenreuth, the county which was hit hardest Germany spring 2020 and early 2021. Of 4203 individuals aged 14 years or older enrolled at baseline (BL, June 2020), 3546 participated FU1 (November 2020) 3391 FU2 (April 2021). Key metrics comprising standardized seroprevalence, surveillance detection ratio (SDR), infection fatality (IFR) success of vaccination...
SARS-CoV-2 seroprevalence was reported as substantially increased in medical personnel and decreased smokers after the first wave spring 2020, including our population-based Tirschenreuth Study (TiKoCo). However, it is unclear whether these associations were limited to early pandemic decrease due reduced infection or antibody response. We evaluated association of occupation smoking with period-specific seropositivity: for until July 2020 (baseline, BL), low period summer (follow-up 1, FU1,...
In a previous study, we described highly significant association between reactogenicity and SARS-CoV-2 RBD IgG titers wild-type neutralization capacity in males after basic vaccination with BNT162b2. The objective of this study was to assess whether benefit long lasting also evident BNT162b2 booster vaccination. Reactogenicity classified into three groups: no or minor injection site symptoms, moderate (not further classified) severe adverse reactions (defined as any symptom(s) resulting sick...
Abstract Approximately 75% of the UK population has received only one dose a 2-dose COVID-19 vaccine regime in face circulating SARS-CoV-2 Variants Concern (VOCs). We aimed to determine levels vaccine-induced neutralising antibodies variants B.1.1.7, B.1.351 and P.1. To do so, we undertook single-centre cross-sectional study health care workers (HCWs) outpatients with immunodeficiencies (IDP) based at same critical tertiary NHS Trust, following single either BNT162b2 or AZD1222 vaccines....
Background: Children are affected rather mildly by the acute phase of COVID-19, but predominantly in children and youths, potentially severe life threatening pediatric multiorgan immune syndrome (PMIS) occurs later on. To identify at risk early on, we searched for antibodies against SARS-CoV-2 mild symptoms PMIS those with high levels antibodies. Methods: In a cross-sectional design, aged 1-17 were recruited through primary care pediatricians study (a), if they had an appointment regular...