A5103127331- Ruminant Nutrition and Digestive Physiology
- RNA and protein synthesis mechanisms
- Rangeland Management and Livestock Ecology
- RNA Research and Splicing
- Indigenous Studies and Ecology
- Demographic Trends and Gender Preferences
- CRISPR and Genetic Engineering
- Viral Infections and Immunology Research
- Composting and Vermicomposting Techniques
- Reproductive System and Pregnancy
- Viral gastroenteritis research and epidemiology
- interferon and immune responses
- Amyotrophic Lateral Sclerosis Research
- RNA modifications and cancer
- Congenital Heart Disease Studies
- Reproductive Physiology in Livestock
- MicroRNA in disease regulation
- Advanced biosensing and bioanalysis techniques
- Tracheal and airway disorders
- Neurogenetic and Muscular Disorders Research
- SARS-CoV-2 and COVID-19 Research
- Pregnancy and preeclampsia studies
- Air Quality and Health Impacts
- Animal Virus Infections Studies
- Reproductive Health and Contraception
Jiangxi University of Traditional Chinese Medicine
2025
Huazhong University of Science and Technology
2025
Xiamen University
2025
Qilu Hospital of Shandong University
2024
Yale University
2019-2024
Tibet Academy of Agricultural and Animal Husbandry Sciences
2023
Henan Agricultural University
2019-2023
Baokang Hospital Affiliated to Tianjin University of Traditional Chinese Medicine
2023
Women's Hospital, School of Medicine, Zhejiang University
2019-2023
Northeast Institute of Geography and Agroecology
2023
SARS-CoV-2 is a betacoronavirus with single-stranded, positive-sense, 30-kilobase RNA genome responsible for the ongoing COVID-19 pandemic. Although population average structure models of were recently reported, there little experimental data on native structural ensembles, and most structures lack functional characterization. Here we report secondary heterogeneity entire in two lines infected cells at single nucleotide resolution. Our results reveal alternative conformations across critical...
Translation of open reading frame 1b (ORF1b) in severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) requires a programmed -1 ribosomal frameshift (-1 PRF) promoted by an RNA pseudoknot. The extent to which SARS-CoV-2 replication may be sensitive changes PRF efficiency is currently unknown. Through unbiased, reporter-based high-throughput compound screen, we identified merafloxacin, fluoroquinolone antibacterial, as inhibitor for SARS-CoV-2. Frameshift inhibition merafloxacin robust...
Summary SARS-CoV-2 is a betacoronavirus with single-stranded, positive-sense, 30-kilobase RNA genome responsible for the ongoing COVID-19 pandemic. Currently, there are no antiviral drugs proven efficacy, and development of these treatments hampered by our limited understanding molecular structural biology virus. Like many other viruses, structures in coronaviruses regulate gene expression crucial viral replication. Although transcriptome data were recently reported, to date little...
Abstract Expansion of an intronic (GGGGCC) n repeat region within the C9orf72 gene is a main cause familial amyotrophic lateral sclerosis and frontotemporal dementia (c9ALS/FTD). A hallmark c9ALS/FTD accumulation misprocessed RNAs, which are often targets cellular RNA surveillance. Here, we show that decay mechanisms involving upstream frameshift 1 (UPF1), including nonsense-mediated (NMD), inhibited in brains cultured cells expressing either two arginine-rich dipeptide repeats (R-DPRs),...
CRISPR/Cas9-mediated knock-in (KI) technology allows for easier fluorescent-protein tagging in zebrafish (Danio rerio), a preferred model organism vivo imaging due to its transparency during the early developmental stage. Here, we provide detailed protocol performing high-efficiency fluorescence gene KI, rapid screening KI founders, and low-abundance protein tracing larvae, which will lay critical foundation subsequent physio-pathological studies zebrafish. The current includes complete...
Abstract With the growing prevalence of dual‐earner families in China, fertility decisions are increasingly influenced by experiences work domain. While previous research has explored relationship between static occupational status and intentions, it largely overlooked how career development affects these dynamics. Changes alter both material resources available for childbearing balance family life. Using diagonal reference models on pooled data from Chinese General Social Survey, this study...
Congenital heart defect is the leading malformation in China. There may have been changes congenital incidence because of birth policy shift China over past years. This study aimed to investigate epidemiology, prenatal diagnosis, and outcomes disease improve medical decisions.
Embryo implantation and trophoblast invasion are principal limiting factors of pregnancy establishment. Aberrant embryo development or improper differentiation may lead to various unfavorable pregnancy-related outcomes, including early loss (EPL). Our clinical data show that the serum BMP2 levels were significantly increased during first trimester expression lower in women with EPL than normal pregnancies. Moreover, we observed was expressed oocytes cells cleaved embryos blastocysts prior...
β-Ga2O3 is considered an attractive candidate for next-generation high-power electronics due to its large band gap of 4.9 eV and high breakdown electrical field 8 MV/cm. However, the relatively low carrier concentration electron mobility in β-Ga2O3-based device limit application. Herein, high-quality single crystal with doping ∼3.2 × 1019 cm-3 was realized using optical float-zone method through Ta doping. In contrast SiO2/β-Ga2O3 gate stack structure, we used hexagonal boron nitride as...
Hashimoto's thyroiditis (HT) is an autoimmune disorder with unclear molecular mechanisms. While current diagnosis well-established, understanding of the gut-thyroid axis in HT remains limited. This study aimed to uncover novel signatures by integrating gut metagenome and host transcriptome data (miRNA/mRNA), potentially elucidating disease pathogenesis identifying new therapeutic targets. We recruited 31 early patients 30 healthy controls a two-stage (discovery validation). Blood fecal...
Translation of open reading frame 1b (ORF1b) in severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) requires programmed -1 ribosomal frameshifting (-1 PRF) promoted by an RNA pseudoknot. The extent to which SARS-CoV-2 replication may be sensitive changes PRF efficiency is currently unknown. Through unbiased, reporter-based high-throughput compound screen, we identified merafloxacin, a fluoroquinolone antibacterial, as inhibitor SARS-CoV-2. Frameshift inhibition merafloxacin robust...
Many positive-strand RNA viruses, including all known coronaviruses, employ programmed -1 ribosomal frameshifting (-1 PRF) to regulate the translation of polycistronic viral RNAs. However, only a few host factors have been shown PRF. Through genome-wide CRISPR-Cas9 knockout screen, we identified that either suppress or enhance severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) Among them, eukaryotic initiation factor 2A (eIF2A) specifically and directly enhances PRF independent...
This study evaluated demographic profiles and prevalence of serologic markers among donors who used confidential unit exclusion (CUE) to assess the effectiveness CUE guide public policies regarding use for enhancing safety versus jeopardizing blood supply by dropping CUE.We conducted a cross-sectional analysis whole donations at large center in São Paulo from July 2007 through June 2009, compared data, confirmed results have never (CUE never).There were 265,550 units collected 181,418 2009....
Objective China's family planning program focuses on younger women. Women older than 50 years, especially those in rural areas, are ineligible for free services and often have few other care options. Few studies examined the sexual reproductive health demands unmet needs of these Methods This cross-sectional, interviewer-administered survey included 1,652 women aged to 64 years from seven provinces China. We women's status, knowledge, service demands, whether they still had an intrauterine...
Abstract Background Partner infection is a significant factor in preventing mother-to-child syphilis transmission. We compared pregnancy outcomes between discordant and concordant couples. Methods conducted retrospective study among 3076 syphilis-positive women who received screening together with their partners during pregnancy. Multivariate analysis was used to explore risks for abnormal objects correcting the major covariate factors. Adjusted odds ratios (OR) 95% confidence intervals (CI)...
A dual-functional microRNA probe was constructed for identification of targets and regulation function through light control.