A5052529245- Respiratory and Cough-Related Research
- Asthma and respiratory diseases
- Neuroscience of respiration and sleep
- Ion Channels and Receptors
- Gastroesophageal reflux and treatments
- Neuropeptides and Animal Physiology
- Voice and Speech Disorders
- Receptor Mechanisms and Signaling
- Olfactory and Sensory Function Studies
- Nicotinic Acetylcholine Receptors Study
- Biochemical Analysis and Sensing Techniques
- Ion channel regulation and function
- Dysphagia Assessment and Management
- Pediatric health and respiratory diseases
- Infant Health and Development
- Air Quality and Health Impacts
- Medical and Biological Ozone Research
- Heart Rate Variability and Autonomic Control
- Respiratory viral infections research
- Pharmacological Receptor Mechanisms and Effects
- Plant-based Medicinal Research
- Vagus Nerve Stimulation Research
- Pharmacological Effects and Assays
- Chronic Obstructive Pulmonary Disease (COPD) Research
- Climate Change and Health Impacts
Johns Hopkins University
2014-2023
Johns Hopkins Medicine
2013-2023
Johns Hopkins Bayview Medical Center
2001-2021
McMaster University
2021
Wythenshawe Hospital
2021
University of Manchester
2014-2021
AstraZeneca (United Kingdom)
2021
Montefiore Medical Center
2014-2021
Albert Einstein College of Medicine
2014-2021
Helix (United States)
2021
We have identified the tracheal and laryngeal afferent nerves regulating cough in anaesthetized guinea-pigs. Cough was evoked by electrical or mechanical stimulation of mucosa, citric acid applied topically to trachea larynx. By contrast, neither capsaicin nor bradykinin challenges larynx cough. Bradykinin histamine administered intravenously also failed evoke Electrophysiological studies revealed that majority capsaicin-sensitive neurones (both Adelta- C-fibres) innervating rostral their...
In the epithelium of lower airways, a cell type unknown function has been termed “brush cell” because distinctive ultrastructural feature, an apical tuft microvilli. Morphologically similar cells in nose have identified as solitary chemosensory responding to taste stimuli and triggering trigeminal reflexes. Here we show that brush mouse trachea express receptors (Tas2R105, Tas2R108), downstream signaling molecules (α-gustducin, phospholipase C β2 ) bitter transduction, synthesis packaging...
Reports from individual centres suggest a preponderance of females with chronic cough. Females also have heightened cough reflex sensitivity. Here we reviewed the age and sex unselected referrals to 11 clinics. To investigate cause any observed dimorphism, functional magnetic resonance imaging putative was analysed in normal volunteers. The demographic profile consecutive patients presenting evaluated. Cough challenge capsaicin undertaken volunteers construct concentration-response curve....
Cough initiated from the trachea and larynx in anaesthetized guinea‐pigs is mediated by capsaicin‐insensitive, mechanically sensitive vagal afferent neurones. Tachykinin‐containing, capsaicin‐sensitive C‐fibres also innervate airways have been implicated cough reflex. Capsaicin‐sensitive nerves act centrally synergistically to modify reflex bronchospasm airway mechanoreceptor stimulation. The hypothesis that polymodal mechanoreceptors similarly interact regulate coughing was addressed this...
We have identified a distinct subtype of airway vagal afferent nerve that plays an essential role in regulating the cough reflex. These afferents are exquisitely sensitive to punctate mechanical stimuli, acid, and decreases extracellular chloride concentrations, but insensitive capsaicin, bradykinin, histamine, adenosine, serotonin, or changes intraluminal pressures. In this study we used intravital imaging, retrograde neuronal tracing, electrophysiological analyses characterize structural...
The cold-sensitive cation channel TRPM8 is a target for menthol, which used routinely as cough suppressant and an additive to tobacco food products. Given that cold temperatures menthol activate neurons through gating of TRPM8, it unclear how actively suppresses cough. In this study we describe the antitussive effects (-)-menthol in conscious anesthetized guinea pigs. pigs, evoked by citric acid applied topically tracheal mucosa was suppressed only when selectively administered vapors upper...
Neuroplasticity of bronchopulmonary afferent neurons that respond to mechanical and chemical stimuli may sensitize the cough reflex. Afferent drive in is carried by vagus nerve, vagal nerve terminals have been well defined animals. Yet, both unmyelinated C fibers particularly morphologically distinct, myelinated, nodose-derived mechanoreceptors described animals are poorly characterized humans. To date there no distinctive molecular markers or detailed morphologies available for human...
Abstract For protection from inhaled pathogens many strategies have evolved in the airways such as mucociliary clearance and cough. We previously shown that protective respiratory reflexes to locally released bacterial bitter “taste” substances are most probably initiated by tracheal brush cells (BC). Our single‐cell RNA‐seq analysis of murine BC revealed high expression levels cholinergic taste signaling transcripts ( Tas2r108 , Gnat3 Trpm5 ). directly demonstrate secretion acetylcholine...