Stilbenes, particularly trans -resveratrol, play a highly relevant defense role in grapevines as phytoalexin is induced response to stress. Metabolism and transport of stilbenes can be conveniently investigated grapevine cell culture since large amounts -resveratrol are accumulated the extracellular medium upon treatment with elicitor methylated cyclodextrin, either alone or combined methyl jasmonate. A proteomic approach on membrane fractions was performed find transporter candidates. The...

Summary Grapevine stilbenes, particularly trans‐resveratrol, have a demonstrated pharmacological activity. Other natural stilbenes derived from resveratrol such as pterostilbene or piceatannol, display higher oral bioavailability and bioactivity than the parent compound, but are far less abundant in sources. Thus, to efficiently obtain these bioactive derivatives, there is need develop new bioproduction systems. cell cultures able produce large amounts of easily recoverable extracellular...

The growing demand for t-resveratrol industrial uses has generated considerable interest in its production. Heterologous resveratrol production plant cell suspensions, apart from requiring the introduction of only one or two genes, advantage high biomass yield and a short cultivation time, thus could be an option large-scale Silybum marianum is source flavonolignan silymarin. Phenylpropanoid synthesis cultures this species can activated by elicitation with methyl jasmonate methylated...

Vitis vinifera cell cultures respond to pathogens and elicitors by synthesising extracellularly accumulating stilbenoid phytoalexins. Large amounts of trans-resveratrol (t-R) are produced when a culture is elicited with methylated cyclodextrins (MBCD), either alone or combined methyl jasmonate (MeJA). t-R transport the extracellular medium, which represents apoplastic space, would place this antifungal defence right in battlefield efficiently fight against pathogen attack. Yet despite their...

Multiple reaction monitoring (MRM) is emerging as a promising technique for the detection and quantification of protein biomarkers in complex biological samples. Compared to Western blotting or enzyme assays, its high sensitivity, specificity, accuracy, assay speed, sample throughput represent clear advantage being approach choice analysis proteins. MRM assays are capable detecting quantifying proteolytic peptides differing mass unique particular proteins, that is, proteotypic peptides,...

Grapevine stilbenes are a family of polyphenols which derive from trans-resveratrol having antifungal and antimicrobial properties, thus being considered as phytoalexins. In addition to their diverse bioactive properties in animal models, they highlight strong potential human health maintenance promotion. Due this relevance, highly-specific qualitative quantitative methods analysis necessary accurately analyze different matrices derived grapevine. Here, we developed rapid, sensitive,...

It is currently possible to transfer a biosynthetic pathway from plant another organism. This system has been exploited the metabolic richness of certain species other plants or even more simple organisms such as yeast bacteria for production high added value compounds. Another application bioconvert substrates into scarcer biologically interesting compounds, piceatannol and pterostilbene. These two resveratrol-derived stilbenes, which have very promising pharmacological activities, are...

Pinostilbene is a monomethyl ether analog of the well-known nutraceutical resveratrol. Both compounds have health-promoting properties, but latter undergoes rapid metabolization and has low bioavailability. O-methylation improves stability bioavailability In plants, these reactions are performed by O-methyltransferases (OMTs). Few efficient OMTs that monomethylate resveratrol to yield pinostilbene been described so far. Here, we report engineering OMT from Vitis vinifera (VvROMT), which...

One of the major intent metabolic engineering in cell culture systems is to increase yields secondary metabolites. Efficient transformation methods are a priority successfully apply cultures plants that produce bioactive or therapeutic compounds, such as Vitis vinifera and Taxus x media. The aim this study was establish reliable method transform non-embryogenic these species. V. cv. Gamay/cv. Monastrell lines media were used for Agrobacterium-mediated using Gateway-compatible Agrobacterium...

Piceatannol is a naturally occurring hydroxylated analogue of the stilbene phytoalexin resveratrol that can be found in grape fruit and derived products. has aroused great interest as it been shown to surpass some human health-beneficial properties including antioxidant activity, several pharmacological activities also bioavailability. The plant biosynthetic pathway piceatannol still poorly understood, which bottleneck for development both defence bioproduction strategies. Cell cultures...

The flag leaf is the most important source of carbohydrate during wheat kernel filling. Around a 75% all sugars stored in come from carbon fixed by this leaf. Terra-Sorb® foliar an L-α-amino acid-based product enzymatic hydrolysis for application with high ratio free to total amino acids. Previous agronomical studies carried out on grassy, horticultural and tree crops have shown that increases photosynthetic plant activity chlorophyll content, promotes rapid recovery stress improves fruit...

ABSTRACT Stilbenes, particularly trans -resveratrol, play a highly relevant defense role in grapevine as phytoalexin induced response to stress. Metabolism and transport of stilbenes can be conveniently investigated cell culture since large amounts -resveratrol are accumulated the extracellular medium upon treatment with elicitor methylated cyclodextrin, either alone or combined methyl jasmonate. Aiming at finding trans-resveratrol transporter candidates proteomic approach on cells membrane...

Here we present a study of the characterization and optimization production trans-Resveratrol (t-R) in grape (Vitis vinifera cv. Gamay) cell cultures elicited with methyl jasmonate (MeJA) dimethyl-β-cyclodextrin (DIMEB). The aim this was to determine influence number factors grapevine culture on t-R level 250 mL shaken flasks that would enable better control bioproduction system when it is upscaled 2 L stirred bioreactor. included optimal growth phase for elicitation, concentration elicitors...

Cocoa, Theobroma cacao L. is one of the main tropical industrial crops.Cocoa has a very high level interfering substances, such as polysaccharides and phenolic compounds that could prevent isolation suitable protein.Efficient methods protein extraction are priority to successfully apply proteomic analyses.We compared evaluated two (A B) tissue preparation for total extract by phenol/SDS protocol.The difference in application was extensively washed dry powder pod were made Method A, whereas...