A5058955468- Allergic Rhinitis and Sensitization
- Food Allergy and Anaphylaxis Research
- Asthma and respiratory diseases
- Contact Dermatitis and Allergies
- Dermatology and Skin Diseases
- Mast cells and histamine
- Monoclonal and Polyclonal Antibodies Research
- Plant Reproductive Biology
- Entomological Studies and Ecology
- Transgenic Plants and Applications
- Insect and Pesticide Research
- Immunotherapy and Immune Responses
- Chronic Myeloid Leukemia Treatments
- Celiac Disease Research and Management
- Occupational exposure and asthma
- IL-33, ST2, and ILC Pathways
- Urticaria and Related Conditions
- Veterinary Equine Medical Research
- Indoor Air Quality and Microbial Exposure
- Studies on Chitinases and Chitosanases
- Glutathione Transferases and Polymorphisms
- Immune Cell Function and Interaction
- Healthcare and Venom Research
- Lichen and fungal ecology
- Health and Medical Studies
Medical University of Vienna
2014-2023
Vienna General Hospital
2000-2018
Christian Doppler Laboratory for Thermoelectricity
2010-2017
University of Vienna
1996-2006
Hôpitaux Universitaires de Strasbourg
2000-2004
Karolinska University Hospital
2004
Research Institute of General Pathology and Pathophysiology, the Russian Academy of Medical Sciences
2000-2002
Inserm
2000
Institute of General and Experimental Biology
1991-1999
Institutes of Science and Development
1998
IgE-mediated allergy affects >25% of the population in industrialized countries. Repeated contact with disease-eliciting allergens induces rises allergen-specific IgE Abs and progression disease to more severe manifestations. Our study uses a type vaccine that is based on genetically modified allergen derivatives treat allergic patients. We developed hypoallergenic major birch pollen allergen, Bet v 1, by genetic engineering vaccinated pollen-allergic patients (n = 124) double-blind,...
Abstract The house dust mite (HDM) Dermatophagoides pteronyssinus is one of most important allergen sources and a major elicitor allergic asthma. We screened D. expression cDNA library with IgE Abs from HDM patients. A coding for new was isolated, which showed sequence homology to peritrophins, contain chitin-binding domains are part the peritrophic matrix lining gut arthropods. mature Der p 23 expressed in Escherichia coli as an 8-kDa protein without its hydrophobic leader purified...
A novel approach to reduce the anaphylactic activity of allergens is suggested. The strategy makes use presence conformational immunoglobulin E (IgE) epitopes on one most common allergens. three dimensional structure major birch pollen allergen, Bet v 1, was disrupted by expressing two parts 1 cDNA representing amino acids 1-74 and 75-160 in Escherichia coli. In contrast complete recombinant fragments showed almost no allergenicity exhibited random coil conformation as analyzed circular...
Summary Background Mites belong to the most frequent and potent allergen sources. Immunotherapy with mite extracts is frequently performed if avoidance not possible or successful. However, highly controversial results have been reported for mite‐specific immunotherapy. Objective The aim of this study was develop diagnostic concepts that may contribute an improved selection patients immunotherapy Der p be used immunological monitoring undergoing treatment. Methods IgE reactivity profiles...
Abstract Background House dust mites (HDM) Dermatophagoides pteronyssinus are a frequent indoor allergen source. Our aim was to determine the frequencies of IgE reactivity purified HDM molecules in mite allergic patients from different parts Europe order establish an panel for diagnosis allergy. Materials and methods Populations D. ‐allergic Austria ( n = 56), France 55), Italy 67) Sweden 65) storage 31) were analysed eight natural (n) recombinant (r) allergens (nDer p 1, rDer 2, nDer 4, 5,...
<b><i>Background:</i></b> Diagnosis and immunotherapy of house-dust mite (HDM) allergy is still based on natural allergen extracts. The aim this study was to analyze commercially available <i>Dermatophagoides pteronyssinus</i> extracts from different manufacturers regarding composition content whether variations may affect their allergenic activity. <b><i>Methods:</i></b> Antibodies specific for several <i>D. pteronyssinus...
House dust mites (HDMs) represent one of the most important inducers respiratory allergies worldwide.We sought to investigate IgE and IgG reactivity profiles a comprehensive panel HDM allergens in children with allergic asthma compare them those nonasthmatic atopic children.Sera from clinically well-characterized asthmatic allergy (n = 105), 53), nonatopic 53) were analyzed for 7 (nDer p 1, rDer 2, 5, 7, 10, 21, 23) by means allergen microarray technology.Asthmatic more frequently showed an...
Since mite allergens are the most relevant inducers of allergic diseases worldwide, resulting in significant morbidity and increased burden on health services, International Collaboration Asthma, Allergy Immunology (iCAALL), formed by American Academy Allergy, Asthma (AAAAI), College (ACAAI), European Clinical (EAACI), World Organization (WAO), has proposed to issue an Consensus (ICON) clinical consequences hypersensitivity. The objectives this document highlight aspects biology that...
House dust mites (HDMs) are among the most important allergen sources containing many different allergenic molecules. Analysis of patients from a double-blind, placebo-controlled allergen-specific immunotherapy (AIT) study indicated that may benefit AIT to extents depending on their molecular sensitization profiles.Our aim was investigate in real-life setting whether stratification with HDM allergy according analysis enhance success.Serum and nasal secretion samples (n = 24) (at baseline, 7,...
Abstract Background Understanding differences in sensitization profiles at the molecular allergen level is important for diagnosis, personalized treatment and prevention strategies allergy. Methods Immunoglobulin E (IgE) were determined more than 2800 sera from children nine population‐based cohorts different geographical regions of Europe; north [BAMSE (Sweden), ECA (Norway)], west/central [PIAMA (the Netherlands), BiB United Kingdom), GINIplus (Germany)], south [INMA Sabadell Gipuzkoa...
Allergic disorders are caused by a combination of hereditary and environmental factors. The hygiene hypothesis postulates that early-life microbial exposures impede the development subsequent allergic disease. Recently developed “wildling” mice genetically identical to standard laboratory specific pathogen–free (SPF) but housed under seminatural conditions have rich from birth. Thus, comparing conventional SPF with wildlings, we can uncouple impact lifelong genetic factors on immune...
Abstract We isolated a cDNA encoding major grass pollen allergen from timothy (Phleum pratense) expression library using allergic patients' IgE. The complete encoded an that binds IgE about 80% of pollen-allergic patients. Significant sequence homology was found to other allergens Kentucky bluegrass (Poa as well rye (Lolium perenne) which originally were believed form different identities. Using monoclonal and polyclonal antibodies raised against group V we identified the recombinant protein...
In this study we reinvestigated the kinetics of allergen release from birch pollen (Betula verrucosa) and timothy grass (Phleum pratense) using different protein extraction procedures, immunoblotting with specific antibodies immune electron microscopy. Pollen allergens such as major allergen, Bet v I, allergens, Phl p I V, group-II/III profilins were released rapidly in large amounts hydrated pollen. Within a few minutes could be detected aqueous supernatants prepared serum IgE or...
In order to establish a test system for grass pollen allergy based on the use of recombinant allergens we chose timothy (Phleum pratense), widely spread grass, as model. From lambda gtll cDNA expression library that had constructed from RNA (P. obtained with serum IgE pollen-allergic individual 60 IgE-binding clones. By differential testing sera different patients, selected three distinct clones encoding Phl p I (group I), V V) and profilin which when used together allowed diagnosis in 97...
Summary Background House dust mites (HDMs) represent significant indoor allergen sources for patients with atopic dermatitis (AD). Subcutaneous allergen‐specific immunotherapy (SCIT) has been shown to be successful in allergic rhinitis and mild asthma might an attractive therapeutic option the long‐term treatment of HDM sensitizations AD patients. However, only a few studies have conducted on effectiveness SCIT AD, resulting controversial clinical results. Data immunological changes induced...
Tropomyosins represent clinically relevant seafood allergens but the role of mite tropomyosin, Der p 10, in house dust (HDM) allergy has not been studied detail.To express and purify a recombinant 10 with equivalent IgE reactivity as natural to evaluate its allergenic activity HDM-allergic patients.rDer was expressed Escherichia coli, purified characterized by mass spectrometry circular dichroism. It tested for 1322 patients. Detailed IgE-reactivity profiles six HDM (Der 1, 2, 5, 7, 21) were...