A5043497621- Allergic Rhinitis and Sensitization
- Food Allergy and Anaphylaxis Research
- Asthma and respiratory diseases
- Contact Dermatitis and Allergies
- Plant Virus Research Studies
- Dermatology and Skin Diseases
- Plant-Microbe Interactions and Immunity
- Indoor Air Quality and Microbial Exposure
- Monoclonal and Polyclonal Antibodies Research
- Transgenic Plants and Applications
- Plant tissue culture and regeneration
- Inhalation and Respiratory Drug Delivery
- Mast cells and histamine
- T-cell and B-cell Immunology
- Plant Disease Resistance and Genetics
- Immunodeficiency and Autoimmune Disorders
- Biomedical and Chemical Research
- Bacteriophages and microbial interactions
- Chromosomal and Genetic Variations
Medical University of Vienna
2015-2025
Max Perutz Labs
2003-2007
Vienna Biocenter
2003-2006
Tallinn University of Technology
2006
University of Vienna
2003
House dust mites (HDMs) are among the most important allergen sources containing many different allergenic molecules. Analysis of patients from a double-blind, placebo-controlled allergen-specific immunotherapy (AIT) study indicated that may benefit AIT to extents depending on their molecular sensitization profiles.Our aim was investigate in real-life setting whether stratification with HDM allergy according analysis enhance success.Serum and nasal secretion samples (n = 24) (at baseline, 7,...
Abstract The movement protein of tobacco mosaic virus, MP30, mediates viral cell-to-cell transport via plasmodesmata. complex MP30 intra- and intercellular distribution pattern includes localization to the endoplasmic reticulum, cytoplasmic bodies, microtubules, plasmodesmata likely requires interaction with plant endogenous factors. We have identified analyzed an MP30-interacting protein, MPB2C, from host Nicotiana tabacum. MPB2C constitutes a previously uncharacterized...
<i>Background:</i> Commercial skin prick test (SPT) extracts used for the diagnosis of dog allergy are prepared by extracting allergens from natural sources, e.g. hair and dander. Due to different starting material extraction methods used, it is likely that differ regarding their allergen contents. <i>Methods:</i> The total protein content composition SPT 5 European manufacturers were compared silver-stained SDS-PAGE. Specific antibody probes generated detect major...
We investigated the prevalence of sensitization to cat lipocalin Fel d 7 among 140 cat-sensitized Swedish patients and elucidated its allergenic activity cross-reactivity with dog Can f 1. Sixty-five had IgE rFel whereof 60 also rCan A moderate correlation between levels 1 was found. activated basophils in vitro inhibited binding 4 13 patients, whereas patients. showed high similarities protein structure epitopes common were found using cross-reactive antisera. is a allergen population that...
Abstract Background In the house dust mite (HDM) Dermatophagoides pteronyssinus, Der p 1, 2, 5, 7, 21, and 23 have been identified as most important allergens. The aim of this study was to define hypoallergenic peptides derived from sequences six allergens use complete antibody, T cell, cytokine responses in sensitized nonsensitized subjects. Methods IgE reactivity HDM‐allergic non‐HDM‐sensitized individuals 15 HDM established using ImmunoCAP ISAC technology. Thirty‐three covering were...
The major cat allergen Fel d 1 represents one of the most important respiratory allergens. Aim this study was to engineer recombinant derivatives with reduced IgE reactivity and preserved T cell epitopes for vaccination tolerance induction.Seven mosaic proteins were generated by reassembly non-IgE-reactive peptides which contained sequence elements induction allergen-specific blocking IgG antibodies epitopes. Mosaic expressed in Escherichia coli using codon-optimized synthetic genes compared...
More than 10% of the world's population suffers from an immunoglobulin E (IgE)-mediated allergy to cats which is accompanied mainly by respiratory symptoms such as rhinitis and asthma. Several cat allergen molecules have been identified, but their allergenic activity has not investigated in depth. Purified (Fel d 1, Fel 2, 3, 4, 6, 7 8) were characterized via mass spectrometry circular dichroism spectroscopy regarding molecular fold, respectively. Cat-allergen-specific IgE levels quantified...
Molecular forms of allergen-specific immunotherapy (AIT) for cat allergy are needed. Fel d 1, 4, and 7 the most important allergens. IgE epitopes 4 were mapped by blocking allergic patients' binding with allergen peptide-specific antisera. Five recombinant fusion proteins (PreS-Cat 1-PreS-Cat 5) each containing hepatitis B virus (HBV)-derived PreS as an immunological carrier non-allergenic peptides from sites expressed in Escherichia coli, purified, characterized mass spectrometry, circular...
Abstract Movement protein binding 2C (MPB2C) is a plant endogenous microtubule-associated previously identified as an interaction partner of tobacco (Nicotiana tabacum) mosaic virus movement (TMV-MP). In this work, the role MPB2C in cell-to-cell transport TMV-MP, viral spread TMV, and subcellular localization TMV-MP was examined. To end, plants with reduced levels were generated by gene-silencing strategy. Local systemic TMV unimpaired MPB2C-silenced compared to nonsilenced plants,...
Due to high IgE recognition frequency and allergenic activity, Der p 5 21 are clinically important house dust mite (HDM) allergens. The objective of this study was characterize the immunodominant epitopes responsible for their activity.A panel 12 overlapping peptides spanning sequence were synthesized search sequential by direct testing allergic patients' reactivity. Peptide-specific antibodies raised in rabbits used inhibition studies localizing conformational which visualized on surfaces...