A5015736805- Antimicrobial Resistance in Staphylococcus
- Antibiotic Resistance in Bacteria
- Bacterial biofilms and quorum sensing
- COVID-19 Clinical Research Studies
- Clostridium difficile and Clostridium perfringens research
- SARS-CoV-2 and COVID-19 Research
- Immune Cell Function and Interaction
- Gut microbiota and health
- COVID-19 epidemiological studies
- Asthma and respiratory diseases
- Antibiotics Pharmacokinetics and Efficacy
- Dermatology and Skin Diseases
- Bacterial Identification and Susceptibility Testing
- Cervical Cancer and HPV Research
- Allergic Rhinitis and Sensitization
- Antibiotic Use and Resistance
- COVID-19 and healthcare impacts
- Food Allergy and Anaphylaxis Research
- T-cell and B-cell Immunology
- Colorectal and Anal Carcinomas
- IL-33, ST2, and ILC Pathways
- Antifungal resistance and susceptibility
- Cystic Fibrosis Research Advances
- Heat shock proteins research
- Hepatitis B Virus Studies
University Hospital Münster
2019-2025
Institute of Medical Microbiology and Hygiene
2013-2024
Helmholtz Zentrum München
2019-2023
University of Augsburg
2020-2022
University of Münster
2022
Technical University of Munich
2019-2022
Johannes Gutenberg University Mainz
2015
University of Freiburg
2013
Addenbrooke's Hospital
2013
University of Cambridge
2013
Coronavirus disease 2019 (COVID-19) is a life-threatening respiratory condition caused by severe acute syndrome coronavirus 2 (SARS-CoV-2) and was initially detected in China December 2019. Currently, Germany >140 000 cases of COVID-19 are confirmed. Here we report nosocomial outbreak SARS-CoV-2 infections the pediatric dialysis unit University Hospital Münster (UHM).Single-step real-time reverse-transcription polymerase chain reaction (rRT-PCR) from nasopharyngeal swabs used to diagnose...
Currently, hospitals have been forced to divert substantial resources cope with the ongoing coronavirus disease 2019 (COVID-19) pandemic. It is unclear if this situation will affect long-standing infection prevention practices and impact on healthcare associated infections. Here, we report a nosocomial cluster of vancomycin-resistant enterococci (VRE) that occurred COVID-19 dedicated intensive care unit (ICU) despite intensified contact precautions during current Whole genome sequence-based...
MicroRNAs (MiRs) are small, noncoding RNAs that regulate gene expression posttranscriptionally. In this study, we show MiR-210 is induced by Oct-2, a key transcriptional mediator of B cell activation. Germline deletion results in the development autoantibodies from 5 mo age. Overexpression vivo resulted autonomous expansion B1 lineage and impaired fitness B2 cells. Mice overexpressing exhibited class-switched Ab responses, finding confirmed wild-type cells transfected with mimic. vitro...
Staphylococcus aureus is one of the first and most prevalent pathogens cultured from airways cystic fibrosis (CF) patients, which can persist therefor extended periods. Airway infections in CF patients are characterized by a strong inflammatory response highly recruited neutrophils. One killing mechanism neutrophils formation neutrophil extracellular traps (NETs), capture eradicate bacteria fibers chromatin decorated with antimicrobial granule proteins. S. secretes nuclease, degrade NETs. We...
Abstract Background Microbiome analysis is becoming a standard component in many scientific studies, but also requires extensive quality control of the 16S rRNA gene sequencing data prior to analysis. In particular, when investigating low-biomass microbial environments such as human skin, contaminants distort true microbiome sample composition and need be removed bioinformatically. We introduce MicrobIEM, novel tool bioinformatically remove using negative controls. Results benchmarked...
Antimicrobial resistance (AMR) is a growing threat in healthcare systems, particularly the management of infections critically ill patients. This study highlights how to identify clusters and putative sharing mobile genetic elements, such as transposons, hospital setting using long-read whole genome sequencing (lrWGS). The approach described here can be employed investigate transmission dynamics KPC-3-positive Klebsiella pneumoniae at multiple levels, from entire isolate down individual...
ABSTRACT Dissemination of antimicrobial resistance (AMR) is a growing global public health burden. The aim this study was to characterize AMR plasmid transmissions within tertiary care hospital and identify relevant transmission pathways. During an 18-month observation period, 540 clinical gram-negative multidrug-resistant bacterial (MDRB) isolates were collected during routine surveillance subjected Pacific Biosciences long-read whole genome sequencing. Potential clonal determined based on...
Staphylococcus aureus is one of the major pathogens isolated from airways people with cystic fibrosis (pwCF). Recently, we described a mucoid S. phenotype respiratory specimens pwCF, which constitutively overproduced biofilm that consisted polysaccharide intercellular adhesin (PIA) due to 5bp-deletion (5bp-del) in intergenic region (ica) locus. Since were not able identify 5bp-del isolates two pwCF long-term persistence and number prospective multicenter study, these strains (i)...
Introduction The emergence of extended-spectrum β-lactamase (ESBL)-producing Enterobacteriaceae is an urgent and alarming One Health problem. This study aimed to investigate duplications plasmid-encoded ESBL genes their impact on antimicrobial resistance (AMR) phenotypes in clinical screening isolates. Methods Multi-drug-resistant bacteria from hospitalized patients were collected during routine surveillance January 2022 June 2023, susceptibility patterns determined. Genotypes extracted...
Plasmid transfer is one important mechanism how antimicrobial resistance can spread between different species, contributing to the rise of multidrug resistant bacteria (MDRB) worldwide. Here were present whole genome sequencing (WGS) data two MDRB isolates, an Escherichia coli and a Klebsiella quasipneumoniae, which isolated from single patient. Detailed analysis long-read identified identical F2:A-:B- lncFII plasmid containing blaCTX-M-27 in both suggesting horizontal exchange species. As...
During the current severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic, healthcare systems worldwide have to prevent nosocomial SARS-CoV-2 transmission while maintaining duty of care. In our study, we characterize dynamic in inpatients and workers (HCWs) at University Hospital Münster (UHM) northwest Germany. We identified 27 cases healthcare-associated infections (4 23 HCWs) who had contact with patients and/or HCWs without use adequate PPE. The contacts these index were...
Since March 2020, the COVID-19 pandemic forced hospitals worldwide to intensify their infection control measures prevent health care-associated transmission of SARS-CoV-2. The correct use personal protective equipment, especially application masks, was quickly identified as priority reduce with this pathogen. Here, we report a nosocomial cluster methicillin-resistant Staphylococcus aureus (MRSA) that occurred during in gynecology/obstetrics department, despite these intensified contact...
Water systems in health care facilities can form reservoirs for Gram-negative bacteria. While planning a new neonatal intensive unit (NICU), we performed retrospective evaluation of potential risks from water-diverting on the existing NICU our tertiary University Hospital. During 2017 to 2023, recorded nine nosocomial cluster events with bacterial pathogens NICU. Of these, three clusters bacteria were potentially related sink drains: A Klebsiella oxytoca, Pseudomonas aeruginosa, and an...
With the novel coronavirus-induced disease (COVID-19), there is fear of nosocomial infections and severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) transmissions to healthcare workers (HCW). We report case a 64-year-old male patient who underwent explantation shoulder prosthesis due periprosthetic infection. He was tested SARS-CoV-2 positive 7 days after admission orthopaedic department following strict infection control measures, routinely including screening all patients for...
Abstract Purpose Carbapenemase-producing Enterobacterales (CPE) pose a serious threat for healthcare facilities worldwide, yet the mode of transmission is often unclear. Recently, we recorded an increase bla OXA-48 -harboring isolates at our hospital associated with patients previous medical treatment in Ukraine. We used long-read whole genome sequencing (lrWGS) to characterize these including their plasmids. Methods Samples were collected as part clinical routine diagnostic or screening...
Incidence of anal carcinoma is increased in people living with HIV (PLWH). Due to the improved life expectancy PLWH, identifying appropriate prevention strategies for non-AIDS-defining cancer types such as has become a priority managing PLWH today.We aimed evaluate cytology assessment screening tool dysplasia and/or regardless gender or sexual orientation. Additionally, we investigated correlation between risk factors and abnormal results our patient cohort.People from Interdisciplinary...