A5014165093- T-cell and B-cell Immunology
- Psoriasis: Treatment and Pathogenesis
- Immune Cell Function and Interaction
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Cytokine Signaling Pathways and Interactions
- Cell Adhesion Molecules Research
- Neuroinflammation and Neurodegeneration Mechanisms
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- Immunotherapy and Immune Responses
- Immune Response and Inflammation
- Immune cells in cancer
- Dermatology and Skin Diseases
- Systemic Lupus Erythematosus Research
- Atherosclerosis and Cardiovascular Diseases
- interferon and immune responses
- Cancer Immunotherapy and Biomarkers
- Asthma and respiratory diseases
- Immunodeficiency and Autoimmune Disorders
- Inflammatory mediators and NSAID effects
- Whipple's Disease and Interleukins
- Multiple Sclerosis Research Studies
- Sodium Intake and Health
- Axon Guidance and Neuronal Signaling
- IL-33, ST2, and ILC Pathways
- Phagocytosis and Immune Regulation
Idorsia (Switzerland)
2019-2024
University Medical Center of the Johannes Gutenberg University Mainz
2008-2022
Johannes Gutenberg University Mainz
2006-2022
University of Zurich
2010-2020
Institute for Molecular Medicine
2010-2014
Universitätsklinikum Erlangen
2014
Kiel University
2014
Leipzig University
2014
Saint Louis University
2010
University of Cologne
2008
The conditions leading to the induction of adaptive Foxp3(+) regulatory T cells (T-regs) from peripheral in vivo are incompletely understood. Here, we show that unresponsiveness IL-6 by cell-selective deletion gp130 or immunization wild-type mice with antigen incomplete Freund's adjuvant (IFA), which fails induce IL-6, promotes conversion CD4(+) into T-regs. Thus, both cell-conditional knockout (KO) immunized MOG35-55 complete (CFA) and IFA develop overwhelming antigen-specific T-reg...
Recently T-helper 17 (Th17) cells were demonstrated to disrupt the blood-brain barrier (BBB) by action of IL-17A. The aim present study was examine mechanisms that underlie IL-17A-induced BBB breakdown. Barrier integrity analyzed in murine brain endothelial cell line bEnd.3 measuring electrical resistance values using call impedance sensing technology. Furthermore, in-cell Western blots, fluorescence imaging, and mono-cyte adhesion transendothelial migration assays performed. Experimental...
The clear association of Th17 cells with autoimmune pathogenicity implicates cytokines as critical mediators chronic diseases such EAE. To study the impact IL-17A on CNS inflammation, we generated transgenic mice in which high levels expression could be initiated after Cre-mediated recombination. Although ubiquitous overexpression led to skin inflammation and granulocytosis, T cell-specific did not have a perceptible development health mice. In context EAE, neither cell-driven nor its...
Interleukin (IL)-17A is regarded as an important cytokine to drive psoriasis, inflammatory skin disease marked by increased cardiovascular mortality. We aimed test the hypothesis that overproduction of IL-17A in leading dermal inflammation may systemically cause vascular dysfunction psoriasis-like disease.Conditional overexpression keratinocytes caused severe mice (K14-IL-17A(ind/+) mice), associated with reactive oxygen species formation and circulating CD11b(+) leukocytes blood,...
Abstract Ductal occlusion has been postulated to precipitate focal pancreatic inflammation, while the nature of primary occluding agents remained elusive. Neutrophils make use histone citrullination by peptidyl arginine deiminase-4 (PADI4) in contact particulate extrude decondensed chromatin as neutrophil extracellular traps (NETs). In high cellular density, NETs form macroscopically visible aggregates. Here we show that such aggregates inside ducts humans and mice thereby driving...
Psoriatic arthritis (PsA) is a debilitating immune-mediated inflammatory of unknown pathogenesis commonly affecting patients with skin psoriasis. Here we use complementary single-cell approaches to study leukocytes from PsA joints. Mass cytometry demonstrates 3-fold expansion memory CD8 T cells in the joints compared peripheral blood. Meanwhile, droplet-based and plate-based RNA sequencing paired cell receptor alpha beta chain sequences show pronounced clonal expansions within Transcriptome...
Skin inflammation inhibits bone formation through IL-17A.
IL-17-producing CD4(+) T cells (Th17) have been classified as a new helper cell subset. Using an IL-17 fate mapping mouse strain, which genetically fixes the memory of expression, we demonstrate that IL-17A/F-expressing generated either in vitro or vivo are not stable T-cell Upon adoptive transfer IL-17F-reporter-positive Th17 to RAG-deficient WT animals, encephalitogenic partially lose expression and upregulate IFN-γ. Additionally, show Th1 can convert IL-17A/IFN-γ-coexpressing mesenteric...
Conventional αβ T cells have the ability to form a long‐lasting resident memory T‐cell (T RM ) population in nonlymphoid tissues after encountering foreign antigen. Conversely, concept of ‘innate memory’, where nonadaptive branches immune system deliver rapid, strengthened response upon reinfection or rechallenge, is just emerging. Using T‐cell‐independent Aldara psoriasis mouse model combination with genetic fate‐mapping and reporter systems, we identified subset γδ mice that capable...
Prolonged infections or adjuvant usage can trigger emergency granulopoiesis (EG), leading to dysregulation in neutrophil blood counts. However, the impact of EG on T and B cell function remains largely unknown. In this study, address question, we used a mouse model neutropenia studied immune activation after administration. The initial neutropenic state fostered an environment increased dendritic cell-derived IL-17 production. Interestingly, lysozyme 2-diphtheria toxin A mice exhibited...
CSF-1R haploinsufficiency causes adult-onset leukoencephalopathy with axonal spheroids and pigmented glia (ALSP). Previous studies in the Csf1r+/− mouse model of ALSP hypothesized a central role elevated cerebral Csf2 expression. Here, we show that monoallelic deletion rescues most behavioral deficits histopathological changes mice by preventing microgliosis eliminating microglial transcriptomic alterations, including those indicative oxidative stress demyelination. We also elevation...
Alzheimer’s disease (AD) is accompanied by a robust inflammatory response mediated plaque-associated myeloid cells of the brain. These exhibit altered gene expression profiles and serve as barrier, preventing neuritic dystrophy. The origin these has been controversial therapeutic importance. Here, we genetically labeled different populations unequivocally demonstrated that in AD brain are derived exclusively from resident microglia, with no contribution circulating peripheral monocytes.
Interleukin (IL)-10 is considered a prototypical anti-inflammatory cytokine, significantly contributing to the maintenance and reestablishment of immune homeostasis. Accordingly, it has been shown in intestine that IL-10 produced by Tregs can act on effector T cells, thereby limiting inflammation. Herein, we investigate whether this role also applies cells during central nervous system (CNS) During neuroinflammation, both CNS-resident -infiltrating produce IL-10; yet, as pleotropic function,...
Autoreactive CD4+ T lymphocytes play a vital role in the pathogenesis of multiple sclerosis and its animal model, experimental autoimmune encephalomyelitis. Since discovery helper 17 cells, there is an ongoing debate whether 1, or both subtypes are important for initiation neuroinflammation. We examined peripheral blood cells from patients with active stable relapsing–remitting sclerosis, used mice conditional deletion over-expression transforming growth factor-β inhibitor Smad7, to...