A5100640565- RNA modifications and cancer
- Cancer-related molecular mechanisms research
- MicroRNA in disease regulation
- Cancer Genomics and Diagnostics
- Gene expression and cancer classification
- RNA Interference and Gene Delivery
- Wnt/β-catenin signaling in development and cancer
- Bioinformatics and Genomic Networks
- Data Mining Algorithms and Applications
- Cancer Immunotherapy and Biomarkers
- Face and Expression Recognition
- Evolutionary Algorithms and Applications
- RNA Research and Splicing
- Lung Cancer Treatments and Mutations
- Angiogenesis and VEGF in Cancer
- Circular RNAs in diseases
- TGF-β signaling in diseases
- Immune Cell Function and Interaction
- Cancer, Hypoxia, and Metabolism
- Pancreatic and Hepatic Oncology Research
- Genetic factors in colorectal cancer
- Plant Pathogens and Fungal Diseases
- Bayesian Modeling and Causal Inference
- Cholangiocarcinoma and Gallbladder Cancer Studies
- Molecular Biology Techniques and Applications
University of South China
2024
Wuxi People's Hospital
2023
Nanjing Medical University
2023
National Center of Biomedical Analysis
2022
Hainan Medical University
2021
Hainan Medical College Hospital
2021
Mayo Clinic in Arizona
2021
Soochow University
2012-2020
Wuxi Fourth People's Hospital
2008-2020
Jiangnan University
2020
Tumor mutational burden (TMB), as measured by whole-exome sequencing (WES) or a cancer gene panel (CGP), is associated with immunotherapy responses. However, whether TMB estimated circulating tumor DNA in blood (bTMB) clinical outcomes of remains to be explored.To explore the optimal size and algorithm design CGP for estimation, evaluate reliability, further validate feasibility bTMB actionable biomarker immunotherapy.In this cohort study, named NCC-GP150 was designed virtually validated...
Objective: To determine the role of microRNA 21(miR-21) on left ventricular remodeling rat heart with ischemia-reperfusion (I/R) injury and to investigate underlying mechanism miR-21 mediated myocardium protection.Methods: Rats were randomly divided into three groups: an I/R model group Ad-GFP (Ad-GFP group), Ad-miR-21 (Ad-miR-21 group) a sham-surgery group.Changes in hemodynamic parameters recorded at 1 week after I/R.Histological diagnosis was achieved by hematoxylin eosin (H&E).Left (LV)...
Reactive oxygen species (ROS)-induced cardiac myocyte injury resulting from changes in the expression levels of multiple genes plays a critical role pathogenesis numerous heart diseases. The purpose this study was to determine potential roles microRNA-214 (miR-214) hydrogen peroxide (H2O2)-mediated gene regulation myocytes. In study, we used quantitative real-time RT-PCR (qRT-PCR) demonstrate that miR-214 upregulated myocytes after treatment with H2O2. We transfected cells pre-miR-214...
Abstract Koolen-de Vries syndrome (KdVS) is a rare disorder caused by haploinsufficiency of KAT8 regulatory NSL complex subunit 1 ( KANSL1 ), which characterized intellectual disability, heart failure, hypotonia, and congenital malformations. To date, no effective treatment has been found for KdVS, largely due to its unknown pathogenesis. Using siRNA screening, we identified as an essential gene autophagy. Mechanistic study shows that modulates autophagosome-lysosome fusion cargo degradation...
The aims of the present study were to determine role miR-214 on left ventricular remodeling rat heart with acute myocardial infarction (AMI) and further investigate underlying mechanism miR-214-mediated protection. AMI was induced in which adenovirus-expressing (Ad-miR-214), anti-miR-214, or Ad-GFP had been delivered into rats hearts 4 days prior, while a phosphatase tensin homolog (PTEN) inhibitor administered via intra-peritoneal injection 30 minutes prior AMI. Changes hemodynamic...
While methods for detecting SNVs and indels in circulating tumor DNA (ctDNA) with hybridization capture-based next-generation sequencing (NGS) have been available, copy number variations (CNVs) detection is more challenging. Here, we present a method enabling CNV from 150-gene panel using very low amount of ctDNA. First, read depth-based estimation without paired blood sample was developed cfDNA data healthy people were used to build normal (PoN) model. Then, silico vitro simulations...
Gene selection is an important issue in analyzing multiclass microarray data. Among many proposed methods, the traditional ANOVA F test statistic has been employed to identify informative genes for both class prediction (classification) and discovery problems. However, assumes equal variance. This assumption may not be realistic gene expression paper explores other alternative statistics which can handle heterogeneity of variances. We study five such statistics, include Brown‐Forsythe Welch...
Colorectal cancer (CRC) is one of the most common malignancies in world. Studies have demonstrated that single nucleotide polymorphisms (SNPs) microRNA genes (miRSNPs) are involved occurrence cancers. However, relationship between miRSNPs within terminal-loops precursors and development CRC still largely unknown. In this study, we found a miRSNP rs7911488 T>C terminal-loop pre-miR-1307 was significantly associated with CRC. The C allele more prevalent patients than healthy controls (P <...
Traf2- and Nck-interacting kinase (TNIK) is one of the germinal center family members involved in cytoskeleton organization neuronal dendrite extension. Emerging evidence supports that TNIK essential for activation WNT signaling pathway colon cancer growth. To search novel genetic aberrations drive carcinogenesis, we performed microarray-based comparative hybridization assay gene copy number variations primary tumor samples. Our data showed was amplified 7% (8/106) Chinese gastric patients....
Purpose: Tumor mutational burden (TMB) calculated by whole-exome sequencing (WES) is proved to be effective predict the clinical benefit of immune checkpoint blockades. However, WES not commonly used in China. We aimed determine if a 381-caner-gene panel (CGP) could estimate TMB, delineate landscape TMB Chinese patients and identify mutated genes pathways related higher TMB. Methods: first evaluated correlation between estimated 381-cancer-gene MasterView using data from melanoma sample...
// Yong Mao 1, * , Chengda Zou 2, 3, Fanyi Meng 2 Jiehong Kong Weipeng Wang and Dong Hua 1 Department of Medical Oncology, Institute Cancer, Affiliated Hospital Jiangnan University The Fourth People’s Wuxi, Wuxi 214062, China Center for Drug Metabolism Pharmacokinetics, College Pharmaceutical Sciences, Soochow University, Suzhou 215123, 3 Orthopedics, Children’s These authors contributed equally to this work Correspondence to: Wang, email: wangweipeng@suda.edu.cn Hua,...
Gene expression data from microarrays have been successfully applied to class prediction, where the purpose is classify and predict diagnostic category of a sample by its gene profile. A typical microarray dataset consists levels for large number genes on relatively small samples. As consequence, one basic important question associated with prediction is: how do we identify subset informative contributing most classification task? Many methods proposed but focus two-class problems, such as...
Abstract Gallbladder carcinoma (GBC) is a lethal biliary tract malignant neoplasm. Patient-derived primary cancer cell lines (PDPCs) are appropriate models to explore biological characteristics and potential therapeutics; however, there lack of PDPCs in GBC. In this study, we aimed establish characterize the GBC PDPCs, further investigated intra-tumor heterogeneity (ITH). Multi-region sampling (3–9 regions) operable tumor tissue samples was used PDPCs. Short tandem repeat genotyping for...
It has been reported that upregulation of wingless-type protein 5a (Wnt5a) is associated with poor prognosis in patients non-small cell lung cancer (NSCLC). Wnt5a expression often upregulated radiation-resistant NSCLC cells. However, the biological functions or molecular mechanisms radiosensitivity remain unknown. In present study, MTT assay and flow cytometric analysis were performed to assess effect overexpression knockdown and/or radiation on proliferation apoptosis Furthermore, western...
In this paper we investigate the issues of independence and diversity among individual classifiers participating in a multiple classifier fusion scheme. First present formal definition statistically independent classifiers. Then focus on testing between two Dependence leads to conclusion that every ensemble which they participate is not an Previous studies have argued infuses multi-classifier system, directly related improved performance. Consequently, introduce measure for degree as...