A5026335521- Immune Cell Function and Interaction
- Immunotherapy and Immune Responses
- Immune cells in cancer
- Cancer Immunotherapy and Biomarkers
- Gut microbiota and health
- Epigenetics and DNA Methylation
- T-cell and B-cell Immunology
- interferon and immune responses
- Ferroptosis and cancer prognosis
- RNA modifications and cancer
- Pancreatic and Hepatic Oncology Research
- Cancer-related molecular mechanisms research
- Galectins and Cancer Biology
- RNA Research and Splicing
- Autophagy in Disease and Therapy
- Cancer Research and Treatments
- Phagocytosis and Immune Regulation
- Suicide and Self-Harm Studies
- IL-33, ST2, and ILC Pathways
- Traumatic Brain Injury Research
- Helicobacter pylori-related gastroenterology studies
- Virus-based gene therapy research
- Nanoplatforms for cancer theranostics
- Viral-associated cancers and disorders
- CAR-T cell therapy research
Nanjing Medical University
2013-2025
Wuxi People's Hospital
2023-2025
Jiangsu Cancer Hospital
2019-2024
Huaian First People’s Hospital
2022-2024
Nanyang Technological University
2024
Zhongda Hospital Southeast University
2024
Southeast University
2024
National Institutes of Health Clinical Center
2024
The Fourth People's Hospital
2024
Taizhou People's Hospital
2024
Background and Aims: Type 3 innate lymphoid cells (ILC3s) are essential for host defense against infection tissue homeostasis. However, their role in the development of HCC has not been adequately confirmed. In this study, we investigated immunomodulatory short‐chain fatty acids (SCFAs) derived from intestinal microbiota ILC3 regulation. Approach Results: We report that Lactobacillus reuteri was markedly reduced gut mice with HCC, accompanied by decreased SCFA levels, especially acetate....
Abstract The host microbiota is closely associated with tumor initiation and progression in multiple solid tumors including gastric cancer. aim of this study was to investigate patients cancer whether there are alterations any potential association these may have immune dysregulation. 16S rRNA gene sequencing used analyze 53 mucosal tissue 30 chronic gastritis. effect on the microenvironment (TME) studied by single-cell sequencing, immunohistochemistry, multiplex immunofluorescence staining,...
Objective Patients with increased PD-L1 + host cells in tumours are more potent to benefit from antiprogrammed death-1/programmed death ligand-1 (PD-L1) treatment, but the underlying mechanism is still unclear. We aim elucidate nature, regulation and functional relevance of hepatocellular carcinoma (HCC). Design A total untreated 184 HCC patients was enrolled randomly. C57BL/6 mice given injection Hepa1-6 form autologous hepatoma. ELISpot, flow cytometry real-time PCR applied analyse...
The immune responses during the initiation and invasion stages of human lung adenocarcinoma (LUAD) development are largely unknown. Here, we generated a single-cell RNA sequencing map to decipher dynamics LUAD development. We found that T follicular helper (Tfh)-like cells, germinal center B dysfunctional CD8+ cells increase tumor initiation/invasion form tertiary lymphoid structure (TLS) inside tumor. This TLS starts with an aggregation CD4+ generation CXCL13-expressing Tfh-like followed by...
We have previously discovered a relationship between the low expression of protein tyrosine phosphatase, receptor type O (PTPRO) in tumor-infiltrating T cells and immunosuppression. The aim present study was to investigate decreased PTPRO increased programmed death ligand 1 (PD-L1) both peripheral monocytes macrophages human hepatocellular carcinoma (HCC).The correlation all indices were explored within mice HCC. mechanic regulations studied by using vitro vivo studies.We found significant...
Naturally occurring regulatory T (Treg) cells, which specifically express the transcription factor forkhead box P3 (Foxp3), are engaged in maintenance of immunological self-tolerance and homeostasis. By transcriptional start site cluster analysis, we assessed here how genome-wide patterns DNA methylation or Foxp3 binding sites were associated with Treg-specific gene expression. We found that hypomethylated regions closely Treg up-regulated clusters, whereas had no significant correlation...
Abstract In many types of cancer, tumor cells prefer to use glycolysis as a major energy acquisition method. Here, we found that the 18 fluoro-deoxyglucose (FDG) positron emission tomography (PET)/computed (CT)-based markers were positively associated with expression programmed cell death ligand 1 (PD-L1), pyruvate kinase M2 (PKM2), both which indicate poor prognosis in patients pancreatic ductal adenocarcinoma (PDAC). However, regulatory mechanism PD-L1 remains elusive. this study,...
Innate lymphoid cells (ILCs) are tissue-resident lymphocytes that play critical roles in cytokine-mediated regulation of homeostasis and inflammation. However, relationships between their immune phenotypic characteristics HCC remain largely unexplored.We performed single-cell RNA sequencing analysis on sorted hepatic ILC from human patients with validated using flow cytometry, multiplex immunofluorescence staining, functional experiments. Moreover, we applied selection strategies to enrich...
Background & AimsAlthough the presence of tertiary lymphoid structures (TLS) correlates with positive responses to immunotherapy in many solid malignancies, mechanism by which TLS enhances antitumor immunity is not well understood. The present study aimed investigate underlying cross talk circuits between B cells and tissue-resident memory T (Trm) within understand their role context immunotherapy.MethodsImmunostaining H&E staining chemokine (C-X-C motif) ligand 13 (CXCL13)+ cluster...
Radiotherapy (RT) has been the standard-of-care treatment for patients with glioblastoma (GBM); however, clinical effectiveness is hindered by therapeutic resistance. Here, we demonstrated that tumor immune microenvironment (TIME) exhibited immunosuppressive properties and high expression of Golgi phosphoprotein 3 like (GOLPH3L) in RT-resistant GBM. Our study showed GOLPH3L interacted stimulator interferon genes (STING) at aspartic acid residue 184 after RT, leading to coat protein complex...
Background The immune response within the tumor microenvironment plays a key role in tumorigenesis and determines clinical outcomes of head neck squamous cell carcinoma (HNSCC). However, to date, paucity robust, reliable immune-related biomarkers has been identified that are capable estimating prognosis HNSCC patients. Methods High-throughput RNA sequencing was performed tumors matched adjacent tissues from five patients, signatures expression 730 transcripts selected nCounter PanCancer...
Background Hepatocellular carcinoma (HCC) is the most common pathological type of primary liver cancer. The lack prognosis indicators one challenges in HCC. In this study, we investigated combination tertiary lymphoid structure (TLS) and several systemic inflammation parameters as a indicator for Materials Methods We retrospectively recruited 126 postoperative patients with paraffin section was collected TLS density assessment. addition, from peripheral blood samples. evaluated prognostic...
Abstract Tumor recurrence remains the major cause of management failure after surgical resection glioblastoma (GBM). Immunotherapy has potential to effectively eradicate tumors and trigger immune memory prevent tumor but not improve outcomes GBM patients. Here, an injectable, reactive oxygen species‐degradable therapeutic hydrogel (ADU‐AAV‐PD1@Gel), capable sustained local release soluble PD‐1 (sPD‐1) stimulator interferon gene (STING) agonist ADU‐S100 (ADU), is developed applied in a model....
Abstract B cells secreting IL-10 functionally are recognized as functional regulatory (B reg ) cells; however, direct evidence concerning the phenotype, regulation, and clinical relevance of IL-10-secreting in humans is still lacking. Here, we demonstrate that, although itself anti-inflammatory, + patients with systemic lupus erythematosus (SLE) display aggressive inflammatory features; these features shift their functions away from inducing CD8 T cell tolerance cause them to induce a...
Background Immune-checkpoint inhibitors (ICIs) remain ineffective in a large group of non-small cell lung cancer (NSCLC) patients. Mucosal-associated invariant T (MAIT) cells, population unconventional innate-like lymphocytes abundant the human body, play important roles malignancies. Little is known about immune characteristics MAIT cells NSCLC and correlation with prognosis response rate ICIs treatment. Methods To investigate distribution, activation status, function patients their...
B cells have a previously unrecognized helper function in triggering pathogenic differentiation of inflammatory T H subsets.
Background: In the past decade, immunotherapy has become a major choice for treatment of lung cancer, yet its therapeutic efficacy is still relatively limited due to various immune escape mechanisms tumors. Based on this, we introduce Neo-BCV, novel bacterial composite vaccine designed enhance responses against cancer. Methods: We investigated enhancing effect Neo-BCV through in vivo and vitro experiments, including flow cytometry, RNA-seq, Western blot. Results: have demonstrated that can...
In many solid tumours, including gastric cancer (GC), the beginning and progression of tumour are closely correlated with microbiome. Here, we show changes in microbiota their influence on immune regulation promotion GC through 16s rRNA sequencing single cell RNA sequencing. Streptococcus lutetiensis (S. lutetiensis) was found to be enriched tissues patients. Further analysis using single-cell flow cytometry showed that S. notably affects antitumour immunity by suppressing IL17 signalling...
Glioblastoma (GBM) is the most lethal primary brain tumor with intra-tumoral hierarchy of glioblastoma stem cells (GSCs). The heterogeneity GSCs within GBM inevitably leads to treatment resistance and recurrence. Molecular mechanisms different cellular state remain unclear. Here, we find that classical (CL) mesenchymal (MES) are enriched in reactive immune region high CL-MES signature informs poor prognosis GBM. Through integrated analyses RNA sequencing single-cell datasets, identify...