A5004562287- T-cell and B-cell Immunology
- Acute Myeloid Leukemia Research
- Immune Cell Function and Interaction
- Advanced Fluorescence Microscopy Techniques
- Acute Lymphoblastic Leukemia research
- Cell Image Analysis Techniques
- Immunotherapy and Immune Responses
- Cellular Mechanics and Interactions
- Neuroscience and Neuropharmacology Research
- Chronic Myeloid Leukemia Treatments
- Myeloproliferative Neoplasms: Diagnosis and Treatment
- Microtubule and mitosis dynamics
- Lipid Membrane Structure and Behavior
- Renal and related cancers
- Advanced Biosensing Techniques and Applications
- Photoreceptor and optogenetics research
- Adrenal Hormones and Disorders
- Hippo pathway signaling and YAP/TAZ
- Nanoplatforms for cancer theranostics
- Ubiquitin and proteasome pathways
- Receptor Mechanisms and Signaling
- Single-cell and spatial transcriptomics
- Monoclonal and Polyclonal Antibodies Research
- Advanced biosensing and bioanalysis techniques
- Wnt/β-catenin signaling in development and cancer
Aix-Marseille Université
2012-2024
Inserm
2013-2024
Centre National de la Recherche Scientifique
2010-2024
Laboratoire Adhésion et inflammation
2020-2024
Centre de Recherche en Cancérologie de Marseille
2013-2022
Centre d’Immunologie de Marseille-Luminy
2004-2022
Institut Paoli-Calmettes
2016-2022
Institut Pprime
2017-2022
Physique des interactions ioniques et moléculaires
2016
University of Geneva
2012-2013
The intratumoral microenvironment, or stroma, is of major importance in the pathobiology pancreatic ductal adenocarcinoma (PDA), and specific conditions stroma may promote increased cancer aggressiveness. We hypothesized that this heterogeneous evolving compartment drastically influences tumor cell abilities, which turn PDA aggressiveness through crosstalk mediated by extracellular vesicles (EVs). Here, we have analyzed proteomic stromal signature identified a contribution annexin A6/LDL...
Glutamate receptors are clustered at the membrane through interactions with intracellular scaffolding proteins and cytoskeletal elements but can also be found in compartments or dispersed membrane. This distribution results from an equilibrium between different pools of whose dynamic is poorly known. The group I metabotropic glutamate receptor 5 (mGluR5) concentrated annulus around postsynaptic density large amounts extrasynaptic To analyze stabilization mGluR5, we used single-particle...
Abstract αβT cell development depends upon serial migration of thymocyte precursors through cortical and medullary microenvironments, enabling specialized stromal cells to provide important signals at specific stages their development. Although conventional are subject clonal deletion in the medulla, entry into thymus medulla also fosters differentiation. For example, during postnatal periods, is involved intrathymic generation multiple lineages, notably induction Foxp3+ regulatory T...
In the bone marrow, CXCL12 and IL-7 are essential for B cell differentiation, whereas hematopoietic stem (HSC) maintenance requires SCF CXCL12. Peri-sinusoidal stromal (PSS) cells main source of IL-7, but their characterization as a pro-B niche remains limited. Here, we characterize supporting decipher interaction network allowing retention. Preferential contacts found between PSS cells, which homogeneously express HSC genes. Furthermore, frequently located in vicinity HSCs same niche. Using...
Lymphoid organs exhibit complex structures tightly related to their function. Surprisingly, although the thymic medulla constitutes a specialized microenvironment dedicated induction of T cell tolerance, its three-dimensional topology remains largely elusive because it has been studied mainly in two dimensions using sections. To overcome this limitation, we have developed an automated method for full organ reconstruction three dimensions, allowing visualization intact mouse lymphoid from...
We introduce a series of experimental procedures enabling sensitive calcium monitoring in T cell populations by confocal video-microscopy. Tracking and post-acquisition analysis was performed using Methods for Automated Accurate Analysis Cell Signals (MAAACS), fully customized program that associates high throughput tracking algorithm, an intuitive reconnection routine statistical platform to provide, at glance, the barcode population individual T-cells. Combined with probe, this method...
Replication stress (RS) has a pivotal role in tumor initiation, progression, or therapeutic resistance. In this study, we depicted the mechanism of breast cancer stem cells' (bCSCs) response to RS and its clinical implication. We demonstrated that bCSCs present limited level compared with non-bCSCs patient samples. described for first time spatial nuclear location BMI1 protein triggers cancers. Hence, bCSCs, is rapidly located stalled replication forks recruit RAD51 activate...
Spermatogenesis is a dynamic process that regulated by adhesive interactions between germ and Sertoli cells. Germ cells express the Junctional Adhesion Molecule-C (JAM-C, encoded Jam3), which localizes to germ/Sertoli cell contacts. JAM-C involved in polarity acrosome formation. Using proteomic approach, we demonstrated interacted with Golgi reassembly stacking protein of 55 kDa (GRASP55, Gorasp2) developing Generation study Gorasp2-/- mice revealed knock-out suffered from spermatogenesis...
The thymic medulla is dedicated for purging the T-cell receptor (TCR) repertoire of self-reactive specificities. Medullary epithelial cells (mTECs) play a pivotal role in this process because they express numerous peripheral tissue-restricted self-antigens. Although it well known that formation depends on development single-positive (SP) thymocytes, mechanisms underlying requirement are incompletely understood. We demonstrate here conventional SP CD4⁺ thymocytes bearing autoreactive TCRs...
Receptors for neurotransmitters are concentrated and stabilized at given sites such as synapses through interactions with scaffolding proteins cytoskeletal elements. The transport of receptors first involves directed vesicular trafficking intracellularly stored followed by their targeting to the plasma membrane. Once expressed cell surface, thought reach final location random Brownian diffusion in membrane plane. Here, we investigate whether metabotropic glutamate receptor mGluR5 can also be...
The directed cell migration towards a chemotactic source, chemotaxis, involves three complex and interrelated processes: directional sensing, polarization motility. Directional sensing allows migrating eukaryotic cells to chemotax in extremely shallow gradients (<2% across the body) of chemoattractant. Although has been observed as spatially restricted responses along plasma membrane, our understanding ;compass' that controls gradient-induced translocation proteins during movements is still...
Acute myeloid leukemia (AML) originates from hematopoietic stem and progenitor cells that acquire somatic mutations, leading to disease clonogenic evolution. AML is characterized by accumulation of immature in the bone marrow phenotypic cellular heterogeneity reflective normal differentiation. Here, we show JAM-C expression defines a subset leukemic endowed with leukemia-initiating cell activity (LIC). Stratification de novo patients at diagnosis based on JAM-C-expressing frequencies blood...
Abstract Medullary thymic epithelial cells (mTEC) purge the T cell repertoire of autoreactive thymocytes. Although dendritic (DC) reinforce this process by transporting innocuous peripheral self-antigens, mechanisms that control their entry remain unclear. Here we show mTEC-CD4 + thymocyte crosstalk regulates thymus homing SHPS-1 conventional DCs (cDC), plasmacytoid (pDC) and macrophages. This is controlled lymphotoxin α (LTα), which negatively CCL2, CCL8 CCL12 chemokines in mTECs....
Receptor internalization upon ligand stimulation is a key component of cell's response and allows cell to correctly sense its environment. Novel fluorescent methods have enabled the direct visualization agonist-stimulated G-protein-coupled receptors (GPCR) trafficking in living cells. However, it difficult observe GPCRs vivo due intrinsic autofluorescence cytosolic signals fluorescently labeled GPCRs. This study uses superior positional accuracy single-molecule fluorescence microscopy...
Abstract In the thymus, T lymphocyte repertoire is purged of a substantial portion highly self-reactive cells. This negative selection process relies on strength TCR-signaling in response to self-peptide-MHC complexes, both cortex and medulla regions. However, whether cytokine-signaling contributes remains unclear. Here, we report that, absence Transforming Growth Factor beta (TGF-β) signaling thymocytes, significantly impaired. Highly autoreactive thymocytes first escape cortical acquire...
// Raphaël Bergès 1 , Aurélie Tchoghandjian Arnauld Sergé 2 Stéphane Honoré Dominique Figarella-Branger 3 Felix Bachmann 4 Heidi A. Lane and Diane Braguer Aix-Marseille Univ, CNRS, INP, Inst Neurophysiopathol, Marseille, France Aix Marseille INSERM, Institut Paoli-Calmettes, CRCM, APHM, CHU Timone, 13385, Basilea Pharmaceutica International Ltd., Basel, Switzerland Correspondence to: Braguer, email: diane.braguer@univ-amu.fr Lane,...