We explored the vascular biology of adipose-derived stromal cells (ASCs) from diabetic patients and applied these to a murine ischemic flap model assess comparative angiogenic potentials between normal human ASCs. ASCs were obtained (n = 5) controls 5). Secretion expression cytokines measured under normoxic hypoxic condition in vitro. Conditioned media harvested ASC cultures assessed for their ability stimulate umbilical vein endothelial cell proliferation tubulization. The control injected...

Aging is a biologic process characterized by time-dependent functional declines that are influenced oxidative stress-induced inflammatory reactions. In particular, ultraviolet (UV) irradiation plays key role in cellular senescence photo-aged skin. However, the of epidermal keratinocytes and dermal fibroblasts UV may differ depending on exposure time dosage irradiation. Therefore, purpose study was to evaluate compare effects human (HaCaT) (HDFs). After cell viability test, 200 mJ/cm used...

Sphingolipid metabolites, such as sphingosine and ceramide, are known to play important roles in cell proliferation, differentiation apoptosis, but the physiological of phytosphingosine (PHS) phytoceramide (PHC) poorly understood. In this study we investigated effects PHS, C2-PHC (N-acetylPHS) C6-PHC (N-hexanoylPHS) on growth intracellular signalling enzymes. Treatment Chinese hamster ovary (CHO) cells with or resulted death a time- dose-dependent manner. induced internucleosomal DNA...

Abstract The use of injectable scaffolding materials for in vivo tissue regeneration has raised great interest because it allows cell implantation through minimally invasive surgical procedures. Previously, we showed that poly(lactic-co-glycolic acid) (PLGA) microspheres can be used as an scaffold to engineer cartilage the subcutaneous space athymic mice. purpose this study was determine whether PLGA regenerate hyaline osteochondral defects rabbit knees. A full-thickness wound patellar...

Bone-tendon junction injuries have poor healing potential. This study evaluated the role of TGF-β and BMP-2 in a fibrin glue carrier at bone-tendon junction. Seventy-two skeletally mature male rabbits were divided into 4 groups. The tendo-Achilles was surgically transected its insertion reattached with pullout suture. Group 1 served as control. In groups 2, 3, 4, glue, mixture injected animals sacrificed 8 weeks after surgical procedure. addition to did not significantly improve...

The prevalence of atopic dermatitis (AD) has increased recently all over the world. Several studies worldwide reported growth retardation associated with AD, but few were in Korea. Therefore, objective this study was to identity differences and nutrient intakes between Korean children without AD. participants 71 AD age, gender-matched 81 control aged 10 36 months. Demographic information gathered by questionnaires. Height weight measured at clinic health centers. for height converted as...

Background: Human adipose-derived stromal cells (hASCs) produce various cytokines. Also, there is a growing opinion that large proportion of the useful effects cell therapy may be attributable to secretion Several reports suggested beneficial hASCs on skin. These include antioxidant activity, accelerated wound healing, whitening effects, and antiaging. We investigated effect skin Wnt signaling, which associated with regeneration differentiation. Methods: Pieces human were cocultured hASCs, 2...

Ultraviolet (UV) irradiation affects neuronal structures of the skin and accelerates aging. Cytokine cascades in keratinocytes after UV may result a paracrine inhibitory effect on nerve cells. The purpose present study was to determine direct cytokines induced by radiation cells terms senescence. Our group performed preliminary UV-irradiated keratinocytes. Among 40 studied, granulocyte-macrophage colony-stimulating factor (GM-CSF) increased 4-fold inflammation antibody array. GM-CSF added...

Purpose: The aim of this study was to identify the factors influencing spontaneous decolonization period vancomycin resistant enterococcus (VRE) species in pediatric patients. Methods: medical records patients presenting positive VRE cultures between January 2005 and November 2010 at a tertiary hospital Seoul, Korea, were reviewed retrospectively. subjects divided into two groups according average number days for (325 days). Clinical characteristics compared shorter colonization (

Although therapies with human amnion/chorion are used to ameliorate acute and chronic wounds, it is unclear which component of the tissue promotes wound healing. To characterize comparative effects amnion chorion in healing, we adipose-derived stromal cells assess cell viability, migration, gel contraction after treatment membrane extract (AME) or (CME). We then correlated possible effectors via AME CME protein profiling, compared them by enzyme-linked immunosorbent assay (ELISA), western...

Hepatocyte nuclear factor 4 alpha (HNF4α) is a key master transcriptional for hepatic fat and bile acid metabolic pathways. We aimed to investigate the role of HNF4α in non-alcoholic fatty liver disease (NAFLD). The was evaluated free acid-induced lipotoxicity chenodeoxycholic (CDCA)-induced toxicity. Furthermore, methionine choline deficiency (MCD)-diet-induced NAFLD model. overexpression reduced intracellular lipid contents attenuated palmitic (PA)-induced lipotoxicity. However, protective...

Abstract The present study is based on the concept of neuro-aging and how it may affect surrounding skin cells. It has been shown that many factors play a significant role in homeostasis by interfering with various cytokines, either through activation or inhibition. Granulocyte macrophage colony-stimulating factor (GM-CSF) generally recognized as an inflammatory cytokine, our previous its effects neuronal senescence after ultraviolet (UV) irradiation Following work, this was performed to...