A5102886949- RNA Interference and Gene Delivery
- Advanced biosensing and bioanalysis techniques
- Virus-based gene therapy research
- Antimicrobial agents and applications
- Advanced X-ray and CT Imaging
- Cardiac Imaging and Diagnostics
- Superconducting and THz Device Technology
- Plant and animal studies
- CRISPR and Genetic Engineering
- Advanced Thermodynamic Systems and Engines
- 3D Printing in Biomedical Research
- Bacterial biofilms and quorum sensing
- Polymer Surface Interaction Studies
- Radiation Dose and Imaging
- Adhesion, Friction, and Surface Interactions
- Acoustic Wave Resonator Technologies
- Testicular diseases and treatments
- Radio Astronomy Observations and Technology
- Antimicrobial Peptides and Activities
- Silk-based biomaterials and applications
- Cardiac Structural Anomalies and Repair
- Burkholderia infections and melioidosis
- Nanofabrication and Lithography Techniques
- Cardiac Arrhythmias and Treatments
- Transgenic Plants and Applications
University of Massachusetts Amherst
2016-2023
Chang Gung University
2011-2023
Kaohsiung Chang Gung Memorial Hospital
2011-2023
Taipei Medical University
2021
Cathay General Hospital
2021
National Taiwan University
2016-2020
Amherst College
2018
Institute of Astronomy and Astrophysics, Academia Sinica
2017
Research Center for Applied Science, Academia Sinica
2016-2017
I-Shou University
2016
Genome editing through the delivery of CRISPR/Cas9-ribonucleoprotein (Cas9-RNP) reduces unwanted gene targeting and avoids integrational mutagenesis that can occur strategies. Direct efficient Cas9-RNP into cytosol followed by translocation to nucleus remains a challenge. Here, we report remarkably highly (∼90%) direct cytoplasmic/nuclear Cas9 protein complexed with guide RNA (sgRNA) coengineering carrier nanoparticles. This construct provides effective (∼30%) efficiency opens up...
Abstract Background Oncidium spp. produce commercially important orchid cut flowers. However, they are amenable to intergeneric and inter-specific crossing making phylogenetic identification very difficult. Molecular markers derived from the chloroplast genome can provide useful tools for resolution. Results The complete of economically variety Onc . Gower Ramsey (Accession no. GQ324949) was determined using a polymerase chain reaction (PCR) Sanger based ABI sequencing. length is 146,484 bp....
The rapid emergence of antibiotic-resistant bacterial "superbugs" with concomitant treatment failure and high mortality rates presents a severe threat to global health. superbug risk is further exacerbated by chronic infections generated from biofilms that render them refractory available treatments. We hypothesized efficient antimicrobial agents could be through careful engineering hydrophobic cationic domains in synthetic semirigid polymer scaffold, mirroring amplifying attributes...
Nanocarrier-mediated protein delivery is a promising strategy for fundamental research and therapeutic applications. However, the efficacy of current platforms into cells limited by endosomal entrapment delivered cargo with concomitantly inefficient access to cytosol other organelles, including nucleus. We report here robust, versatile polymeric–protein nanocomposite (PPNC) platform capable efficient (≥90%) proteins cytosol. synthesized library guanidinium-functionalized...
Infections caused by multidrug-resistant (MDR) bacteria are a rapidly growing threat to human health, in many cases exacerbated their presence biofilms. We report here biocompatible oil-in-water cross-linked polymeric nanocomposite that degrades the of physiologically relevant biomolecules. These degradable nanocomposites demonstrated broad-spectrum penetration and elimination MDR bacteria, eliminating biofilms with no toxicity cocultured mammalian fibroblast cells. Notably, serial passaging...
We present here an integrated nanotechnology/biology strategy for cancer immunotherapy that uses arginine nanoparticles (ArgNPs) to deliver CRISPR-Cas9 gene editing machinery into cells generate SIRP-α knockout macrophages. The NP system efficiently codelivers single guide RNA (sgRNA) and Cas9 protein required knock out the "don't eat me signal" in macrophages prevents phagocytosis of cells. Turning off this signal increased innate phagocytic capabilities by 4-fold. This improved attack...
Uncontrolled inflammation is responsible for acute and chronic diseases in the lung. Regulating expression of pro-inflammatory genes pulmonary tissue using small interfering RNA (siRNA) a promising approach to combatting respiratory diseases. However, siRNA therapeutics are generally hindered at cellular level by endosomal entrapment delivered cargo organismal inefficient localization tissue. Here we report efficient anti-inflammatory activity vitro vivo polyplexes an engineered cationic...
Infections caused by bacterial biofilms are an emerging threat to human health. Conventional antibiotic therapies ineffective against due poor penetration of the extracellular polymeric substance secreted colonized bacteria coupled with rapidly growing number antibiotic-resistant strains. Essential oils promising natural antimicrobial agents; however, solubility in biological conditions limits their applications both dispersed (planktonic) and biofilm settings. We report here oil-in-water...
Objectives The purposes of this study were to report the sonographic features superficial epidermal cysts with an emphasis on characteristic pseudotestis appearance and highlight spectrum ancillary findings. Methods medical records studies all cases surgically proven (n = 42) from January 2005 through December 2009 reviewed. Twenty-six (62%) that appeared sonography as ovoid nodules homogeneous low medium echoes, simulating a testicle, included in group. other 16 (38%) without pattern...
Current strategies for the delivery of proteins into cells face general challenges endosomal entrapment and concomitant degradation protein cargo. Efficient directly to cytosol overcomes this obstacle: we report here use biotin-streptavidin tethering provide a modular approach generation nanovectors capable cytosolic biotinylated proteins. This strategy uses streptavidin organize oligo(glutamate) peptide complexes that are then electrostatically self-assembled with cationic...
An unprecedented dual-mode optical switch by combining helicene and bis-azobenzene moieties in proximity allows for cooperative, highly diastereoselective chirochromic (92/8 to 3/97) photochromic (>99/<1 19/81) switchings both solution nematic LC with tunable pitches handedness permutation of four different metastable states.
Cell adhesion is crucial to cell behaviors including survival, growth, and differentiation. In recent years, quartz crystal microbalance with dissipation monitoring (QCM-D) has exhibited advantages in examining real-time viscoelastic changes of surface interactions. Self-assembled monolayers (SAMs) are known for their convenience versatility modifying surfaces. A series ζ-potentials can be obtained by introducing two functional groups opposite charge gold surfaces, namely,...
The presence of aortic arch calcification (AoAC) and cardiomegaly on chest radiography has been demonstrated as important risk factors for cardiovascular mortality in patients with chronic kidney disease (CKD). However, the interrelationship among AoAC, cardiomegaly, renal function progression remains unclear. aim this study is to assess whether AoAC are independently associated stages 3-5 CKD.We retrospectively determined by X-ray 237 patients, followed up at least three years without...
Heat treatment in fluorous solvents generates stable films on 3D substrates, enabling translation of protein molecular properties into film characteristics.
Acute epididymo-orchitis (AEO)-related global testicular infarction (GTI) is rare. We report herein the clinical and ultrasound findings of 6 patients with AEO-related GTI. Seventeen torsion-related GTI were also reviewed compared. The echotexture ranged from mildly inhomogeneous to diffuse heteroechoic, depending on severity necrotic changes. All showed a juxta-epididymal string-of-bead pattern color Doppler ultrasound, which was ascribed patent arteries (5/6, 87%) collateral vessels (1/6,...
Protein-based biomaterials provide versatile scaffolds for generating functional surfaces biomedical applications. However, tailoring the and biological properties of protein films remains a challenge. Here, we describe high-throughput method to designing stable, by combining inkjet deposition inks with nanoimprint lithography based methodology. The translation intrinsically charged proteins into materials was demonstrated through controlled cellular adhesion. This modular strategy offers...
Objective: Heart rate variability (HRV) reflects autonomous nervous system disturbance and is used for seizure prediction. The aim of this study was to develop a real-time, continuous physiological medical signal data acquisition in detection intensive care unit (ICU). Methods: This prospective conducted National Taiwan University Hospital from August 2018 October 2019. included 20 patients who (a) had sustained return spontaneous circulation following out-of-hospital cardiac arrest, (b)...
Nanotechnology for non-viral vaccination is a rapidly advancing field. Supramolecular assembly between plasmid DNA and cationic polymer, PONI-Guan enables efficient transfection in vitro vivo against the Newcastle disease virus.