A5073422497- Osteoarthritis Treatment and Mechanisms
- Tendon Structure and Treatment
- Knee injuries and reconstruction techniques
- Connective Tissue Growth Factor Research
- Exercise and Physiological Responses
- Cardiovascular Effects of Exercise
- Connective tissue disorders research
- MicroRNA in disease regulation
- Sports injuries and prevention
- Cell Image Analysis Techniques
- Gene Regulatory Network Analysis
- RNA and protein synthesis mechanisms
- Single-cell and spatial transcriptomics
- RNA modifications and cancer
- Cellular Mechanics and Interactions
- Periodontal Regeneration and Treatments
- Cell Adhesion Molecules Research
- RNA Interference and Gene Delivery
- RNA Research and Splicing
- Immunotherapy and Immune Responses
- Inflammatory mediators and NSAID effects
- Molecular Biology Techniques and Applications
- Virus-based gene therapy research
- Musculoskeletal synovial abnormalities and treatments
- Heat shock proteins research
University of Oxford
2022-2025
University of Liverpool
2018-2024
Nuffield Orthopaedic Centre
2022-2023
Abstract The molecular and cellular basis of health in human tendons remains poorly understood. Among tendons, hamstring tendon has markedly low pathology can provide a prototypic healthy reference. aim this study was to determine the transcriptomes location all cell types tendon. Using single nucleus RNA sequencing, we profiled 10 533 nuclei from four donors identified 12 distinct types. We confirmed presence two fibroblast types, endothelial cells, mural immune previously unreported...
Osteoarthritis (OA) is a whole joint disease that affects all tissues, with changes in the articular cartilage (AC), subchondral bone and synovium. Pathologies menisci ligaments, however, are rarely analysed, although both known to play vital roles mechanical stability of joint. The aim our study was describe pathological ligament during development murine spontaneous post-traumatic surgically induced OA quantify tissue mineralisation space using micro-computed tomography (μCT) imaging progression.
Abstract Tendon ruptures in humans have often been studied during the chronic phase of injury, particularly context rotator cuff disease. However, early response to acute tendon remains less investigated. Quadriceps tendons, which require prompt surgical treatment, offer a model investigate this response. Therefore, study aimed explore cellular changes ruptured compared healthy human quadriceps tendons. samples were collected from patients undergoing fracture repair (healthy) or surgery...
Abstract Objectives Osteoarthritis (OA) is increasingly recognised as a whole joint disease, with an important role for synovium. However, the repertoire of immune cells and fibroblasts that constitute OA synovium remains understudied. This study aims to characterise cellular composition advanced explore potential correlations between different cell types patient demographics or clinical scores. Methods Synovium, collected from 10 patients during total knee replacement surgery, was...
Aberrant miRNA expression has been associated with a large number of human diseases. Therefore, targeting miRNAs to regulate their levels become an important therapy against diseases that stem from the dysfunction pathways regulated by miRNAs. In recent years, small molecules have demonstrated enormous potential as drugs (i.e., SM-miR). A clear understanding mechanism action on upregulation and downregulation allows precise diagnosis treatment oncogenic pathways. However, outside slow costly...
Abstract Tendon ruptures in humans have regularly been studied during the chronic phase of injury. However, early response to injury remains less investigated. Quadriceps tendons, which require prompt surgical treatment, offer a model investigate this response. Therefore, study aimed explore cellular changes ruptured compared healthy human quadriceps tendons. tendon samples were collected from patients undergoing tibial shaft fracture repair (healthy) or surgery for complete rupture...
Abstract Background Knee joint injuries, common in athletes, have a high risk of developing post-traumatic osteoarthritis (PTOA). Ligaments, matrix-rich connective tissues, play important mechanical functions stabilising the knee joint, and yet their role post-trauma is not understood. Recent studies shown that ligament extracellular matrix structure compromised early stages spontaneous (OA) PTOA, but it remains unclear how pathology affects function. In this study, we aim to investigate...
ABSTRACT CCN2 is a matricellular protein involved in several crucial biological processes. In particular, cartilage development and osteoarthritis. Ccn2 null mice exhibit range of skeletal dysmorphisms, highlighting its importance regulating matrix formation during development; however, role adult remains unclear. The aim this study was to determine the postnatal chondrocytes models post-traumatic osteoarthritis (PTOA). deletion induced articular male transgenic at 8 weeks age. PTOA knees...
The collection of (human) tissue is essential for many aspects biomedical research. In order to maintain quality and achieve reproducible representative results, it critical that the collected in a consistent manner, especially when by different people, at locations, and/or over long period time. This particularly important snap-frozen tissue, which can be stored periods time before use range experiments including bulk single nucleus RNA sequencing imaging. Therefore, this protocol provides...
Abstract Articular soft tissue mineralization and ossification are clear pathological signs of osteoarthritis joints. However their molecular cellular aetiologies remain largely unknown. TGF-ß family members known contributors to both development. In this study, we used a Fibrillin-1 mutant mouse, the Tight Skin mouse (TSK), define detrimental effects abnormal Fbn1 in TSK mice high activity joint pathology such as articular ossification. Knee joints male Female Wild-type (WT) littermates...
Abstract The molecular and cellular basis of health in human tendons remains poorly understood. Amongst tendons, the hamstrings are least likely to be injured or degenerate, providing a prototypic healthy tendon reference. aim this study was define transcriptome location all cell types hamstring tendon. We profiled transcriptomes 10,533 nuclei from 4 donors using single-nucleus RNA sequencing (snRNA-seq) identified 12 distinct types. confirmed presence two fibroblast types, endothelial...
Abstract Osteoarthritis (OA) is a whole joint disease that affects all tissues, with changes in the articular cartilage (AC), subchondral bone and synovium. Pathologies menisci ligaments, however, are rarely analysed, although both known to play vital roles mechanical stability of joint. The aim our study was describe pathological ligament during development murine spontaneous post-traumatic surgically-induced OA quantify tissue mineralisation space using µCT imaging progression. Knees...
Abstract CCN2 is a matricellular protein involved in several critical biological processes. In particular, cartilage development and osteoarthritis. null mice exhibit range of skeletal dysmorphisms, highlighting its importance regulating matrix formation during development, however role adult remains unclear. The aim this study was to determine the postnatal chondrocytes models post-traumatic osteoarthritis (PTOA). deletion induced articular male transgenic at 8 weeks age. PTOA knees either...
Abstract Aberrant miRNA expression has pervasively been found to relate a growing number of human diseases. Therefore, targeting miRNAs regulate their levels become an important therapy against diseases that stem from the dysfunction oncogenic pathways regulated by miRNAs. In recent years, small molecule compounds have demonstrated enormous potential as drugs ( i . e ., SM-miR). A clear understanding mechanism action molecules on down- and up-regulating allows precise diagnosis treatment...
1 Abstract Osteoarthritis (OA) is a whole joint disease that affects all knee tissues. Ligaments, matrix-rich connective tissue, play an important mechanical function stabilises the and yet their role in OA not well studied. Recent studies have shown ligament extracellular matrix (ECM) structure compromised early stages of OA, but it remains unclear how this biomechanics. In study, aim was to investigate structural, cellular viscoelastic changes anterior cruciate (ACL) murine non-invasive...