A5100772631- RNA Research and Splicing
- Cancer-related molecular mechanisms research
- RNA modifications and cancer
- Pluripotent Stem Cells Research
- CRISPR and Genetic Engineering
- Genomics and Chromatin Dynamics
- Nanoplatforms for cancer theranostics
- Immune Cell Function and Interaction
- Ocular Oncology and Treatments
- interferon and immune responses
- RNA regulation and disease
- Epigenetics and DNA Methylation
- T-cell and B-cell Immunology
- Single-cell and spatial transcriptomics
- Skin and Cellular Biology Research
- RNA Interference and Gene Delivery
- Immunotherapy and Immune Responses
- Cytomegalovirus and herpesvirus research
- Genetic Syndromes and Imprinting
- Gene expression and cancer classification
- Microfluidic and Bio-sensing Technologies
- HVDC Systems and Fault Protection
- Natural product bioactivities and synthesis
- Plant biochemistry and biosynthesis
- Genomics and Phylogenetic Studies
Shanghai Jiao Tong University
2021-2025
Ministry of Education of the People's Republic of China
2025
Sichuan Agricultural University
2025
Guangzhou University of Chinese Medicine
2024
Stanford University
2013-2024
Guangdong Academy of Medical Sciences
2024
Guangdong Provincial People's Hospital
2024
Howard Hughes Medical Institute
2013-2014
Chinese Academy of Sciences
2010
Long noncoding RNAs (lncRNAs) are thought to be prevalent regulators of gene expression, but the consequences lncRNA inactivation in vivo mostly unknown. Here, we show that targeted deletion mouse Hotair leads derepression hundreds genes, resulting homeotic transformation spine and malformation metacarpal-carpal bones. RNA sequencing conditional reveal an ongoing requirement repress HoxD genes several imprinted loci such as Dlk1-Meg3 Igf2-H19 without affecting imprinting choice. binds both...
Patients with recessive dystrophic epidermolysis bullosa (RDEB) lack functional type VII collagen owing to mutations in the gene COL7A1 and suffer severe blistering chronic wounds that ultimately lead infection development of lethal squamous cell carcinoma. The discovery induced pluripotent stem cells (iPSCs) ability edit genome bring possibility provide definitive genetic therapy through corrected autologous tissues. We generated patient-derived COL7A1-corrected epithelial keratinocyte...
HOTAIR is a 2.2-kb long noncoding RNA (lncRNA) whose dysregulation has been linked to oncogenesis, defects in pattern formation during early development, and irregularities the process of epithelial-to-mesenchymal transition (EMT). However, oncogenic transformation determined by vivo its impact on chromatin dynamics are incompletely understood. Here, we generate transgenic mouse model with doxycycline-inducible expression human context MMTV-PyMT breast cancer-prone background systematically...
Abstract Significant progress in the application of immune checkpoint blockade (ICB) for treatment multiple types cancers has been achieved, but its overall response rate and therapeutic efficacy remain unsatisfactory. To address these limitations, identification a combinational approach to enhance ICB is needed. The activation cyclic GMP-AMP synthase-stimulator interferon genes (cGAS-STING) signaling critical induction antitumor innate responses promising target development immunotherapy....
Self-renewal and differentiation of embryonic stem cells (ESCs) are controlled by intracellular transcriptional factors extracellular factor-activated signaling pathways. Transcription factor Oct4 is a key player maintaining ESCs in an undifferentiated state, whereas the Erk/MAPK pathway known to be important for ESC differentiation. However, manner which pluripotency modulate pathways not well understood. Here, we report identification target gene Oct4, serine/threonine kinase 40 ( Stk40 ),...
Abstract We present Dystrophic Epidermolysis Bullosa Cell Therapy (DEBCT), a scalable platform producing autologous organotypic iPS cell-derived induced skin composite (iSC) grafts for definitive treatment. Clinical-grade manufacturing integrates CRISPR-mediated genetic correction with reprogramming into one step, accelerating derivation of COL7A1 -edited cells from patients. Differentiation epidermal, dermal and melanocyte progenitors is followed by CD49f-enrichment, minimizing maturation...
Memory T cells exhibit considerable diversity that determines their ability to be protective. Here, we examine whether changes in cell heterogeneity contribute the age-associated failure of immune memory. By screening for age-dependent cell-surface markers, identify CD4 and CD8 memory subsets are unrelated previously defined central effector cells. expressing ecto-5'-nucleotidase CD73 constitute a functionally distinct subset declines with age. They resemble long-lived, polyfunctional but...
Summary Background Gene editing in induced pluripotent stem (iPS) cells has been hailed to enable new cell therapies for various monogenetic diseases including dystrophic epidermolysis bullosa (DEB). However, manufacturing, efficacy and safety roadblocks have limited the development of genetically corrected, autologous iPS cell-based therapies. Methods We developed Dystrophic Epidermolysis Bullosa Cell Therapy (DEBCT), a generation GMP-compatible (cGMP), reproducible, scalable platform...
A. muciniphila (AKK) has attracted extensive research interest as a potential next-generation probiotics, but its role in intestinal pathology is remains unclear. Herein, this study was conducted to investigate the effects of DSM 22,959 on growth performance, barrier function, microecology and inflammatory response weaned piglets stimulated by dextran sulfate sodium salt (DSS). Twenty-four Duroc × Landrace Yorkshire (DLY) used for 2 factorial arrangement treatments were divided into four...
We develop PIRCh-seq, a method which enables comprehensive survey of chromatin-associated RNAs in histone modification-specific manner. identify hundreds several cell types with substantially less contamination by nascent transcripts. Non-coding are found enriched on chromatin and classified into functional groups based the patterns their association specific modifications. find single-stranded RNA bases more chromatin-associated, we discover allele-specific RNA-chromatin interactions. These...
Abstract Tumor-specific neoepitopes are promising targets in cancer immunotherapy. However, the identification of functional tumor-specific remains challenging. In addition to most common source, single-nucleotide variants (SNV), alternative splicing (AS) represents another rich source and can be utilized cancers with low SNVs such as uveal melanoma (UM). UM, prevalent adult ocular malignancy, has poor clinical outcomes due a lack effective therapies. Recent studies have revealed promise...
Abstract Background ARID1A, a subunit of the SWI/SNF chromatin remodeling complex, is thought to play significant role both in tumor suppression and initiation, which highly dependent upon context. Previous studies have suggested that ARID1A deficiency may contribute cancer development. The specific mechanisms whether loss affects tumorigenesis by RNA editing remain unclear. Results Our findings indicate leads an increase levels alterations categories mediated adenosine deaminases acting on...
The quantification of developmental potential is critical for determining stages and identifying essential molecular signatures in single-cell studies. Here, we present FitDevo, a novel method inferring using scRNA-seq data. main idea FitDevo first to generate sample-specific gene weight (SSGW) then infer by calculating the correlation between SSGW expression. generated generalized linear model that combines information learned from training dataset covering data 17 previously published...
Abstract Deciphering cell-type-specific 3D structures of chromatin is challenging. Here, we present InferLoop, a novel method for inferring the strength interaction using single-cell accessibility data. The workflow InferLoop is, first, to conduct signal enhancement by grouping nearby cells into bins, and then, each bin, leverage signals loop newly constructed metric that similar perturbation Pearson correlation coefficient. In this study, have described three application scenarios including...
Abstract Stoliczka’s Asian trident bat ( Aselliscus stoliczkanus ) is a small-bodied species and very sensitive to climate change. Here, we presented chromosome-level genome assembly of A. by combining Illumina sequencing, Nanopore sequencing high-throughput chromatin conformation capture (Hi-C) technology. The was 2.18 Gb in size with 98.26% the sequences anchored onto 14 autosomes two sex chromosomes (X Y). quality high contig scaffold N50 72.98 162 Mb, respectively, Benchmarking Universal...
ABSTRACT Many long noncoding RNAs (lncRNAs) regulate gene transcription through binding to histone modification complexes. Therefore, a comprehensive study of nuclear in modification-specific manner is critical understand their regulatory mechanisms. Here we develop method named Profiling Interacting on Chromatin by deep sequencing (PIRCh-seq), which profile chromatin-associated transcriptome 5 different cell types using antibodies recognizing H3 and 6 distinct modifications associated with...
<title>Abstract</title> <italic>Elephantopus tomentosus</italic> (ET) Linn. was reported to be an anti-tumor plant. However, the chemical composition of ET and its compounds potential mechanisms still unclear. In this paper, UPLC-Q-TOF-MS/MS first used identified ingredients in UPLC determine main ET. Network pharmacology applied predict mechanisms. Anti-tumor nuclear activate targets were obtained anti-liver cancer effect validated on HepG2. Finally, Molecule docking, RT-qPCR, western...
SUMMARY Tissue development results from lineage-specific transcription factors (TF) programming a dynamic chromatin landscape through progressive cell fate transitions. Here, we interrogate the epigenomic during epidermal differentiation and create an inference network that ranks coordinate effects of TF-accessible regulatory element-target gene expression triplets on lineage commitment. We discover two critical transition periods: surface ectoderm initiation keratinocyte maturation,...