A5100448583- Cancer-related gene regulation
- Dermatology and Skin Diseases
- Immune Cell Function and Interaction
- Genomics and Chromatin Dynamics
- RNA Research and Splicing
- RNA modifications and cancer
- Systemic Sclerosis and Related Diseases
- CRISPR and Genetic Engineering
- Herpesvirus Infections and Treatments
- Epigenetics and DNA Methylation
- T-cell and B-cell Immunology
- Single-cell and spatial transcriptomics
- Acne and Rosacea Treatments and Effects
- Galectins and Cancer Biology
- Retinal Imaging and Analysis
- Cutaneous lymphoproliferative disorders research
- Retinal Diseases and Treatments
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Chromosomal and Genetic Variations
- Pluripotent Stem Cells Research
- Acute Myeloid Leukemia Research
- CAR-T cell therapy research
- IL-33, ST2, and ILC Pathways
- Cancer Mechanisms and Therapy
- TGF-β signaling in diseases
University of Electronic Science and Technology of China
2025
Sichuan Cancer Hospital
2025
Stanford University
2014-2024
Howard Hughes Medical Institute
2002-2024
Xuzhou Medical College
2023
Dynamic Imaging (United Kingdom)
2020
Peking University Cancer Hospital
2002
University of Hong Kong
2002
Peking University
2002
Gastric cancer is the world's second most common cause of death. We analyzed gene expression patterns in 90 primary gastric cancers, 14 metastatic and 22 nonneoplastic tissues, using cDNA microarrays representing approximately 30,300 genes. cancers were distinguished from tissues by characteristic differences their patterns. found a diversity cancer, reflecting variation intrinsic properties tumor normal cells cellular composition these complex tissues. identified several genes whose levels...
Rosacea is a common, chronic skin disease that currently incurable. Although environmental factors influence rosacea, the genetic basis of rosacea not established. In this genome-wide association study, discovery group 22,952 individuals (2,618 cases and 20,334 controls) was analyzed, leading to identification two significant single-nucleotide polymorphisms (SNPs) associated with one which replicated in new 29,481 (3,205 26,262 controls). The confirmed SNP, rs763035 (P=8.0 × 10(-11) group;...
Long noncoding RNAs are thought to regulate gene expression by organizing protein complexes through unclear mechanisms. XIST controls the inactivation of an entire X chromosome in female placental mammals. Here we develop and integrate several orthogonal structure-interaction methods demonstrate that RNA-protein complex folds into evolutionarily conserved modular architecture. Chimeric clustered binding fRIP eCLIP experiments align with long-range RNA secondary structure, revealing discrete...
Cells communicate with each other via receptor-ligand interactions. Here, we describe lentiviral-mediated cell entry by engineered interaction (ENTER) to display ligand proteins, deliver payloads, and record receptor specificity. We optimize ENTER decode interactions between T (TCR)-MHC peptides, antibody-antigen, pairs. A viral presentation strategy allows capture B any antigen. engineer genetic payloads antigen-specific or cells selectively modulate cellular behavior in mixed populations....
Genome-wide association studies (GWASs) of eye disorders have identified hundreds genetic variants associated with ocular disease. However, the vast majority these are noncoding, making it challenging to interpret their function. Here we present a joint single-cell atlas gene expression and chromatin accessibility adult human retina more than 50,000 cells, which used analyze single-nucleotide polymorphisms (SNPs) implicated by GWASs age-related macular degeneration, glaucoma, diabetic...
We analyzed gene expression patterns in human gastric cancers by using cDNA microarrays representing ≈30,300 genes. Expression of PLA2G2A, a previously implicated as modifier the Apc Min/+ (multiple intestinal neoplasia 1) mutant phenotype mouse, was significantly correlated with patient survival. confirmed this observation an independent set samples quantitative RT-PCR. Beyond its potential diagnostic and prognostic significance, result suggests intriguing possibility that activity PLA2G2A...
The Xist lncRNA mediates X chromosome inactivation (XCI). Here we show that Spen, an Xist-binding repressor protein essential for XCI , binds to ancient retroviral RNA, performing a surveillance role recruit chromatin silencing machinery these parasitic loci. Spen loss activates subset of endogenous (ERV) elements in mouse embryonic stem cells, with gain accessibility, active histone modifications, and ERV RNA transcription. directly RNAs structural similarity the A-repeat Xist, region...
Abstract Systemic sclerosis (SSc) is a disease at the intersection of autoimmunity and fibrosis. However, epigenetic regulation contributions diverse cell types to SSc remain unclear. Here we survey, using ATAC-seq, active DNA regulatory elements eight primary cells in normal skin from healthy controls, as well clinically affected unaffected patients. We find that accessible skin-resident dendritic (DCs) exhibit highest enrichment SSc-associated single-nucleotide polymorphisms (SNPs) predict...
The blood-brain barrier (BBB) is primarily manifested by a variety of physiological properties brain endothelial cells (ECs), but the molecular foundation for these remains incompletely clear. Here, we generate comprehensive atlas adult ECs using acutely purified mouse and integrated multi-omics. Using RNA sequencing (RNA-seq) proteomics, identify transcripts proteins selectively enriched in demonstrate that they are partially correlated. single-cell RNA-seq, dissect basis functional...
Human dendritic cells (DCs) comprise subsets with distinct phenotypic and functional characteristics, but the transcriptional programs that dictate their identity remain elusive. Here, we analyze global chromatin accessibility profiles across resting stimulated human DC by means of assay for transposase-accessible using sequencing (ATAC-seq). We uncover specific regions each subset regulators function. By comparing plasmacytoid responses to IFN-I-producing non-IFN-I-producing conditions,...
ABSTRACT Genome-wide association studies (GWAS) of eye disorders have identified hundreds genetic variants associated with ocular disease. However, the vast majority these are noncoding, making it challenging to interpret their function. Here, we present a joint single-cell atlas gene expression and chromatin accessibility adult human retina >50,000 cells, which used analyze noncoding single-nucleotide polymorphisms (SNPs) implicated by GWAS age-related macular degeneration, glaucoma,...
While much is known about genes that promote aging, little protect against or prevent particularly in human skin. The main objective of this study was to perform an unbiased, whole transcriptome search for associate with intrinsic skin youthfulness. To accomplish this, healthy women (n = 122) European descent, ages 18-89 years Fitzpatrick type I/II were examined facial aging parameters and clinical covariates, including smoking ultraviolet exposure. Skin youthfulness defined as the top 10%...