A5037249717- Retinal Development and Disorders
- Retinal Diseases and Treatments
- Retinal and Optic Conditions
- Neuroinflammation and Neurodegeneration Mechanisms
- Corneal Surgery and Treatments
- Neuroscience and Neuropharmacology Research
- Glaucoma and retinal disorders
- Neuroscience and Neural Engineering
- Photoreceptor and optogenetics research
- Pluripotent Stem Cells Research
- Retinal Imaging and Analysis
- bioluminescence and chemiluminescence research
- Retinal and Macular Surgery
- Ion channel regulation and function
- Mesenchymal stem cell research
- Complement system in diseases
- Ocular and Laser Science Research
- Optical Coherence Tomography Applications
- Zebrafish Biomedical Research Applications
- Receptor Mechanisms and Signaling
- Retinoids in leukemia and cellular processes
- Neurogenesis and neuroplasticity mechanisms
- Immunotherapy and Immune Responses
- Advanced Fluorescence Microscopy Techniques
- Drug-Induced Ocular Toxicity
University Hospital of Bern
2016-2025
University of Bern
2016-2025
University of Glasgow
2022-2023
University of Colorado Anschutz Medical Campus
2023
UCB Pharma (Germany)
2022
UCB Pharma (United Kingdom)
2022
Institute of Infection and Immunity
2022
Center for Systems Biology
2022
Massachusetts General Hospital
2022
University Hospital Regensburg
2021
To compare central retinal thickness (CRT) measurements in healthy eyes by different commercially available OCT instruments and to the intersession reproducibility of such measurements.Six (Stratus [Carl Zeiss Meditec, Inc. Dublin, CA], SOCT Copernicus [Reichert/Optopol Technology, Inc., Depew, NY], Spectral OCT/SLO [Opko/OTI, Miami, FL], RTVue-100 [Optovue Corp., Fremont, Spectralis HRA+OCT [Heidelberg Engineering, Heidelberg, Germany], Cirrus HD-OCT Inc.]) were used assess CRT both...
Microglia are the resident tissue macrophages of central nervous system including retina. Under pathophysiological conditions, microglia can signal to Müller cells, major glial component retina, affecting their morphological, molecular, and functional responses. Microglia-Müller cell interactions appear be bidirectional shaping overall injury response in Hence, responses disease have been ascribed both positive negative outcomes. However, reactivity survival absence immune cells after not...
To investigate the appearance of geographic atrophy in high-resolution optical coherence tomography (OCT) images, fundus autofluorescence (FAF) pattern, and infrared images simultaneously recorded with a novel combined OCT-scanning laser ophthalmology (SLO) system.Patients aged over 50 years secondary to dry age-related macular degeneration (ARMD) were assessed prospective cross-sectional study by means simultaneous spectral OCT-SLO (Spectralis HRA+OCT; Heidelberg Engineering, Heidelberg,...
Quantification of retinal layers using automated segmentation optical coherence tomography (OCT) images allows for longitudinal studies and neurological disorders in mice. The purpose this study was to compare the performance layer algorithms with data from manual mice Spectralis OCT.Spectral domain OCT 55 three different mouse strains were analyzed total. scans 22 C57Bl/6, BALBc, 11 C3A.Cg-Pde6b+Prph2Rd2 /J automatically segmented commercially available compared segmentation.Fully performed...
Age-related macular degeneration (AMD), one of the leading causes blindness worldwide, personal suffering and high socioeconomic costs. While there has been progress in treatments for neovascular form AMD, no therapy is yet available more common dry form, also known as geographic atrophy. We analysed retinal tissue a mouse model caused by sodium iodate (NaIO
The cellular events that dictate the initiation of complement pathway in ocular degeneration, such as age-related macular degeneration (AMD), is poorly understood. Using gene expression analysis (single cell and bulk), mass spectrometry, immunohistochemistry, we dissected role multiple retinal choroidal types determining homeostasis. Our scRNA-seq data show response to early AMD more robust choroid, particularly fibroblasts, pericytes endothelial cells. In late AMD, changes were prominent...
ABSTRACT Glia antigen‐presenting cells (APCs) are pivotal regulators of immune surveillance within the retina, maintaining tissue homeostasis and promptly responding to insults. However, intricate mechanisms underlying their local coordination activation remain unclear. Our study integrates an animal model retinal injury, retrospective analysis human retinas, in vitro experiments gain insights into crucial role antigen presentation neuroimmunology during degeneration (RD), uncovering...
purpose. To correlate damage to the retinal pigment epithelium (RPE) with decreased visual function after systemic administration of sodium iodate (NaIO3). methods. Damage was produced in mice by injection 15, 25, or 35 mg/kg NaIO3. Visual assessed cued water maze (WM) behavioral test and optokinetic reflex (OKR) measurement at different times injection. Autofluorescence whole eye flatmounts quantified, hematoxylin eosin staining paraffin sections performed assess changes outer retina....
Abstract Background The aim of this study was to evaluate the visual acuity adult zebrafish by assessing optokinetic reflex. Using a modified commercially available optomotor device (OptoMotry®), virtual three-dimensional gratings variable spatial frequency or contrast were presented zebrafish. In first experiment, evaluated changing at different angular velocities. Thereafter, sensitivity level frequencies. Results At tested velocities (10, 15, 20, 25, and 30 d/s) 100%, values ranged from...
Herein, we have investigated retinal cell-death pathways in response to the retina toxin sodium iodate (NaIO3) both vivo and vitro. C57/BL6 mice were treated with a single intravenous injection of NaIO3 (35 mg/kg). Morphological changes post comparison untreated controls assessed using electron microscopy. Cell death was determined by TdT-mediated dUTP-biotin nick end labeling (TUNEL) staining. The activation caspases calpain measured immunohistochemistry. Additionally, cytotoxicity...
To characterize chemoattractants expressed by the retinal pigment epithelium (RPE) after sodium iodate (NaIO3)-induced damage and to investigate whether ocular-committed stem cells preexist in bone marrow (BM) migrate response chemoattractive signals damaged RPE.C57/BL6 mice were treated with a single intravenous injection of NaIO3 (50 mg/kg) create RPE damage. At different time points real-time RT-PCR, ELISA, immunohistochemistry used identify secreted subretinal space. Conditioned medium...
Blue-light fundus autofluorescence (FAF) imaging is currently widely used for assessing dry age-related macular degeneration (ARMD). However, at this wavelength, the fovea appears as circular zone of marked hypofluorescence, due to absorption pigment (MP). This dark spot could be misinterpreted an atrophic area and lead difficulties in identifying small, central changes. The purpose study was analyze differences image quality, FAF patterns, lesion size, when using conventional blue-light...
Oxidative stress-induced damage of the retinal pigment epithelium (RPE) and chronic inflammation have been suggested as major contributors to a range diseases. Here, we examined effects oxidative stress on endogenous complement components proinflammatory angiogenic responses in RPE cells. ARPE-19 cells exposed for 1–48 h H2O2 had reduced cell–cell contact increased markers epithelial–mesenchymal transition but showed insignificant cell death. Stressed expression receptors CR3 (subunit CD11b)...
Contrasting with zebrafish, retinal regeneration from Müller cells (MCs) is largely limited in mammals, where they undergo reactive gliosis that consist of a hypertrophic response and ultimately results vision loss. Transforming growth factor β (TGFβ) essential for wound healing, including both scar formation regeneration. However, targeting TGFβ may affect other physiological mechanisms, owing its pleiotropic nature. The regulation various cellular activities by relies on interaction...
Retinal degeneration is a disease affecting the eye, which an immune-privileged site because of its anatomical and physiological properties. Alterations in retinal homeostasis-because injury, disease, or aging-initiate inflammatory cascades, where peripheral leukocytes (PL) infiltrate parenchyma, leading to degeneration. So far, research on PL's role was limited observing few cell types at specific times sectioning tissue. This restricted our understanding immune interactions response...
Abstract Background Retinal degeneration results from disruptions in retinal homeostasis due to injury, disease, or aging and triggers peripheral leukocyte infiltration. Effective immune responses rely on coordinated actions of resident microglia recruited macrophages, critical for tissue remodeling repair. However, these phagocytes also contribute chronic inflammation degenerated retinas, yet the precise coordination response damage remains elusive. Recent investigations have demonstrated...
The aim of the study was to directly compare threshold electrical charge density retina (retinal threshold) in rabbits for generation evoked potentials (EEP) by delivering stimulation with a custom-made microelectrode array (MEA) implanted into either subretinal or suprachoroidal space. Nine eyes seven Dutch-belted were studied. electroretinogram (ERG), visual (VEP) and EEP recorded. Electrodes VEP placed on dura mater overlying cortex. recorded following MEA subretinally beneath streak...
Purpose.: The purpose of this study was to analyze predictive factors for best-corrected visual acuity (BCVA) after anti-VEGF treatment in patients with macular edema (ME) secondary central retinal vein occlusion (CRVO). Methods.: This prospective enrolled treatment-naive ME CRVO. BCVA, ophthalmoscopy, fundus photography, and spectral domain optical coherence tomography (SD-OCT) imaging were performed. SD-OCT analyzed integrity the external limiting membrane (ELM), photoreceptor inner...