The bulb of Lilium brownii F. E. Brown (Liliaceae) (LB) is a common Chinese medicine to relieve insomnia.To investigate the molecular mechanism LB relieving insomnia.Insomnia model was induced by intraperitoneally injection p-chlorophenylalanine (PCPA) in Wistar rats. Rats were divided into three groups: Control, PCPA (400 mg/kg, i.p. 2 days), (598.64 oral 7 days). levels 5-hydroxytryptamine (5-HT), norepinephrine (NE), melatonin (MT), and expression GABAA, 5-HT1A MT receptors, as well...

Context Lilium davidii var. unicolour Cotton (Lilium genus, Liliaceae) is an edible plant and a herb used in China to alleviate insomnia.Objective To investigate the alleviating insomnia mechanism of L. (LD).Materials methods Wistar rats were intraperitoneally injected with p-chlorophenylalanine (PCPA) establish model. Rats divided into six groups (n = 8): Control, PCPA, Estazolam (0.5 mg/kg), LD extract low, medium high doses (185.22, 370.44, 740.88 mg/kg). Serum hormone levels HPA axis,...

In the study, an aqueous two phase system (ATPS) coupled with ultrasound was employed to extract lignans from Zanthoxylum armatum. Three standard lignans, namely (−)-fargesin, sesamin and L-asarinin, were used as marker compounds, extraction optimized projected by response surface methodology (RSM) artificial neural network (ANN). The optimal condition for ATPS 20% n-propanol 24% (NH4)2SO4 ultrasonic-assisted including a solvent solid ratio of 15:1, temperature 40 °C, treatment time 55 min...

Abstract Psoriasis is a common immune‐mediated inflammatory skin disease, caused by disturbed interactions between keratinocytes and immune cells. Chinese medicine shows potential clinical application for its treatment. Liquiritin flavone compound extracted from licorice antitussive, antioxidant antiinflammatory effects, therefore may have as psoriasis therapeutic. The aim of this work was to examine the possible roles that liquiritin in treating psoriasis. HaCaT cells were stimulated TNF‐α...

Insomnia is the second most prevalent mental illness worldwide. Periostracum cicadae (PC), as an animal traditional Chinese medicine with rich pharmacological effects, has been documented a treatment for children's night cries, and later extended to treat insomnia. This study aimed investigate effects of PC extract N-acetyldopamine compounds in ameliorating The UPLC-ESI-QTOF-MS analysis determined that mainly contained components. Previously, we also isolated some acetyldopamine polymers...

The dried fruits of Alpinia oxyphylla Miq have been widely used as an herbal medicine for the treatment diarrhea and enuresis in China. Medicinal chemistry studies revealed that tepenes, diphenylheptanes flavones were main components. Therefore, these three kinds components should be chosen bioactive marker compounds quality control A. fruits. Moreover, multiple active has recognized to a more feasible method medicines. This study firstly provided better comprehensive component analysis...

Context The root of Potentilla anserina L. (Rosaceae) is an herbal medicine that has been used as antitussive and expectorant drug for thousands years in Chinese folk medicine.Objective: This study estimated the effects P. extract to validate its traditional use.Materials methods activities ethanol extract, aqueous polysaccharides from were evaluated using classical animal models.Results results showed three tests, at high low doses significantly inhibited frequency cough induced by ammonia...

D-Allulose 3-epimerase (DAE) can convert D-fructose into D-allulose, but its lower activity and poor thermal stability restrict application in industrial production. This study utilized a semi-rational design specific site mutations to enhance the enzyme of DAE from Clostridium scindens ATCC 35704 (Csc-DAE) at same time. Notably, activities six mutants (V22I, A25V, Q46I, C212N, G165A, G267D) orderly superimposed double-point were increased by 1.08–1.31 1.28–1.64 times that wild type (553.7...

Enzyme catalysis represents a promising approach for sustainable chemical synthesis, yet its industrial applications face limitations due to the inefficient regeneration and high cost of essential cofactors, such as adenosine-5′-triphosphate (ATP) nicotinamide adenine dinucleotide phosphate (NADPH). While natural metabolic systems efficiently recycle cofactors through spatially organized enzymes, replicating this efficiency in vitro remains challenging. Here, we prepare five-enzyme...

Rhubarb contains biologically active compounds such as anthraquinones, anthrones, stilbenes and tannins. A rapid efficient UPLC/Q-TOF-MS/MS method was developed applied towards identifying the constituents of Rheum tanguticum Maxim. ex Balf. for first time. Chemical were separated investigated by in negative ion mode. The ESI-MS2 fragmentation pathways four types interpreted, providing a very useful guidance characterisation different compounds. Based on exact mass information,...

We studied the relationship between pressure ratio of buffer gases (argon and neon) rate coherent population trapping resonance frequency shift with cell temperature in Rb85. found that when total varies within range 5–15kPa, along a bell shaped curve. Every curve crossed horizontal axis at two points are roughly symmetrical respect to midpoint 1:1. This allows us minimize by adjusting these points.

A new lignan glycoside, (7S,8R)-guaiacylglycerol-ferulic acid ether-7-O-β-D-glucopyranoside (1), along with five known phenylpropanoids (2-6) and seven phenylpropanoid glycosides (7-13), were isolated from the stems of Zanthoxylum armatum DC. Their structures elucidated by spectroscopic analysis. Compounds 2-8 11-13 first Rutaceae others for time Z. armatum.

In this study, the selenium enriched peanuts and different solubility proteins extracted from them were investigated. The dried defatted (SeP) powder (0.3147 μg/g) had a 2.5-fold higher mean total concentration than general (GP) power (0.1233 μg/g). SeP of selenium, manganese zinc that GP, but less calcium. rate extraction protein was 23.39% for alkali soluble main component in SeP, which accounted 92.82% combined 77.33% protein. forms WSePr due to DPPH free-radical scavenging activity,...

Abstract Quinolone antibiotic residues may pose potential threat to human health. A rapid and sensitive method was developed for the determination of quinolone in serum urine. After solid phase extraction (SPE) process, eight were analyzed by high-performance liquid chromatography/tandem mass spectrometry (HPLC-MS/MS) using ciprofloxacin-d8 as internal standard. The relative standard deviation intra-day inter-day precision quinolones less than 7.52% accuracies ranged from 95.8% 103% serum,...

We proposed a scheme to implement coherent population trapping (CPT) atomic clock based on the transient CPT phenomenon. proved that transmitted laser power in typical Λ system near resonance features as damping oscillation. Also, oscillating frequency is exactly equal detuning from hyperfine splitting. Therefore, we can directly measure and then compensated output of microwave oscillator get standard frequency. By this method, further simplify structure clock, make it easier be digitized...