A5058723075- Fatty Acid Research and Health
- Immunotherapy and Immune Responses
- T-cell and B-cell Immunology
- Immune Response and Inflammation
- Immune Cell Function and Interaction
- Lipid Membrane Structure and Behavior
- MicroRNA in disease regulation
- Immune cells in cancer
- RNA Interference and Gene Delivery
- Peroxisome Proliferator-Activated Receptors
- Eicosanoids and Hypertension Pharmacology
- Psoriasis: Treatment and Pathogenesis
- Microbial Natural Products and Biosynthesis
- Immunodeficiency and Autoimmune Disorders
- Lipid metabolism and biosynthesis
- Intensive Care Unit Cognitive Disorders
- Hemodynamic Monitoring and Therapy
- Mechanical Circulatory Support Devices
- Cardiac Arrest and Resuscitation
- RNA Research and Splicing
- Cancer-related molecular mechanisms research
- Diet, Metabolism, and Disease
- Cellular transport and secretion
- Phagocytosis and Immune Regulation
- IL-33, ST2, and ILC Pathways
University Hospital in Halle
2015-2024
Humboldt-Universität zu Berlin
2017-2021
Charité - Universitätsmedizin Berlin
2013-2021
Freie Universität Berlin
2017-2021
Martin Luther University Halle-Wittenberg
2018-2020
Leipzig University
2009-2014
University of Veterinary Medicine
2011
Molecular Research Institute
2011
Medizinische Hochschule Hannover
2009
University of Würzburg
2008-2009
Sequence analyses of fungal genomes have revealed that the potential fungi to produce secondary metabolites is greatly underestimated. In fact, most gene clusters coding for biosynthesis antibiotics, toxins, or pigments are silent under standard laboratory conditions. Hence, it one major challenges in microbiology uncover mechanisms required pathway activation. Recently, we discovered intimate physical interaction important model fungus Aspergillus nidulans with soil-dwelling bacterium...
Analysis of the first gene cluster encoding cytochalasan biosynthesis and targeted RNA silencing in Penicillium expansum revealed that these complex tricyclic natural products are assembled by a fungal PKS-NRPS hybrid synthase, CheA. A biosynthetic Diels−Alder macrocyclization can be rationalized on basis deduced enzyme functions.
The family of NFAT (nuclear factor activated T-cells) transcription factors plays an important role in cytokine gene regulation. In peripheral T-cells NFATc1 and -c2 are predominantly expressed. Because different promoter poly(A) site usage as well alternative splicing events, is synthesized multiple isoforms. highly inducible NFATc1/A contains a relatively short C terminus, whereas the longer, constitutively expressed isoform NFATc1/C spans extra C-terminal peptide 246 amino acids....
CD44 is a prominent activation marker which distinguishes memory and effector T cells from their naïve counterparts. It also plays role in early cell signaling events as it bound to the lymphocyte-specific protein kinase thereby enhances receptor signalling. Here, we investigated whether IFN-γ IL-17 producing helper differ expression dependence of for differentiation. Stimulation CD4+ with allogeneic dendritic resulted formation three distinguishable populations: CD44+, CD44++ CD44+++. In...
Transfection of suspension cells has proven to be very difficult using conventional methods. Here, we present a simple and time-saving new transfection protocol wherein cell culture plates coated with chicken egg white are seeded prior transfection. Our results demonstrate that coupling coatings commercially available reagents leads high efficiency lines including canine mastocytoma C2 the human myeloid line HL-60. This approach, which should prove applicable wide range lines, solves crucial...
The eukaryotic cell membrane possesses numerous complex functions, which are essential for life. At this, the composition and structure of lipid bilayer particular importance. Polyunsaturated fatty acids may modulate physical properties biological membranes via alteration affecting physiological processes, e.g. in immune system. In this systematic study we present acid peptide profiles rafts murine macrophages that have been supplemented with saturated as well PUFAs from n-3, n-6 n-9 family....
Significance T helper type (Th) 9 cells demonstrate both pro- and antiinflammatory properties, pointing to a functional heterogeneity not examined so far. Applying single cell gene expression analysis of alloreactive Th9 cells, we revealed the existence two major subsets, CD96 high low with strongly opposing inflammatory and, especially, colitis-inducing potential. Mechanistically, found that controls cytokine potential providing strong evidence for an inhibitory role in controlling CD4 +...
Alteration of miRNAs and dietary polyunsaturated fatty acids (PUFAs) underlies vascular inflammation. PUFAs are known to be incorporated into the cell membrane monocytes/macrophages or endothelial cells, major cellular players diseases, thereby affecting signal transduction. Nevertheless, there no investigations concerning PUFA impact on miRNA expression by these cells. With regard key role play for overall functionality, this study aims elucidate whether affect profiles. To end,...
Nutritional fatty acids are known to have an impact on membrane lipid composition of body cells, including cells the immune system, thus providing a link between dietary acid uptake, inflammation and immunity. In this study we reveal significance macrophage gene expression cytokine synthesis thereby highlighting signal transduction processes, activation as well defense mechanisms. Using RAW264.7 macrophages model identified polyunsaturated (PUFA) both n-3 n-6 family down-regulate of: (i)...
Acute graft-versus-host disease (GVHD) occurs in 40% to 60% of recipients partially matched umbilical cord blood transplantation (UCBT). In a phase I study, adoptive transfer expanded CD4(+)CD25(+)Foxp3(+) natural regulatory T cells (nTregs) resulted reduced incidence grade II-IV acute GVHD. To investigate potential mechanisms responsible for the GVHD risk, we analyzed peripheral mononuclear cell mRNA expression tolerance gene set previously identified operation- tolerant kidney transplant...
It is well known that PUFA impede the LPS-mediated activation of transcription factor NFkappaB. However, underlying mode action has not been clarified yet. To address this issue in a comprehensive approach, we used monocyte/macrophage cell line RAW264.7 to investigate consequences supplementation on TLR4 pathway with focus (i) gene expression itself as its downstream mediators, (ii) membrane microdomain localization and CD14, (iii) stimulation-induced interaction CD14. Our data indicate...
Profound alterations in the lipid profile of raft and non‐raft plasma membrane microdomains were found when RAW264.7 macrophages supplemented with polyunsaturated fatty acids (PUFAs) physiologically relevant concentrations. For first time lipids detergent‐free isolated domains phagocytic immune cells characterized by mass spectrometry. The extent remodeling differed different n3 n6 PUFA supplements. mildest effects detected for α‐linolenic acid (LNA) linoleic (LA), C18 precursors families,...
Superinfections are a fundamental critical care problem, and their significance in severe COVID-19 cases needs to be determined. This study analyzed data from the Lean European Open Survey on SARS-CoV-2-Infected Patients (LEOSS) cohort focusing intensive patients. A retrospective analysis of patient 840 with courses demonstrated that co-infections were frequently present primarily nosocomial origin. Furthermore, our showed invasive therapy procedures accompanied an increased risk for...
The transfer of alloreactive regulatory T (aTreg) cells into transplant recipients represents an attractive treatment option to improve long-term graft acceptance. We recently described a protocol for the generation aTreg in mice using nondepleting anti-CD4 antibody (aCD4). Here, we investigated whether adding TGF-β and retinoic acid (RA) or rapamycin (Rapa) can further aTreg-cell function. Murine CD4(+) were cultured with allogeneic B presence aCD4 alone, aCD4+TGF-β+RA aCD4+Rapa. Addition...
Background Toll like receptors (TLRs) are an important and evolutionary conserved class of pattern recognition associated with innate immunity. The Gram-positive cell wall constituents strongly depends on TLR2. In order to be functional, TLR2 predominantly forms a heterodimer TLR1 or TLR6 within specialized membrane microdomains, the lipid rafts. composition physicochemical properties rafts subject modification by exogenous fatty acids. Previous investigations our group provide evidence that...