A5000665467- Respiratory Support and Mechanisms
- Hemodynamic Monitoring and Therapy
- Renal function and acid-base balance
- Cardiac Arrest and Resuscitation
- Receptor Mechanisms and Signaling
- Chronic Obstructive Pulmonary Disease (COPD) Research
- Sepsis Diagnosis and Treatment
- Healthcare Technology and Patient Monitoring
- Intensive Care Unit Cognitive Disorders
- Mechanical Circulatory Support Devices
- Non-Invasive Vital Sign Monitoring
- Protein Kinase Regulation and GTPase Signaling
- Neuropeptides and Animal Physiology
- Cardiac, Anesthesia and Surgical Outcomes
- Atomic and Subatomic Physics Research
- Neonatal Respiratory Health Research
- Monoclonal and Polyclonal Antibodies Research
- Cardiovascular Function and Risk Factors
- Airway Management and Intubation Techniques
- Neuroscience and Neuropharmacology Research
- Inhalation and Respiratory Drug Delivery
- Computational Drug Discovery Methods
- Ultrasound in Clinical Applications
- Ion channel regulation and function
- Heart Failure Treatment and Management
Aalborg University
2016-2025
University Hospitals Plymouth NHS Trust
2024
Places For People
2019
University of Bari Aldo Moro
2017
Novem (Netherlands)
2017
Morpho (United States)
2017
University of Ferrara
2017
Istituti di Ricovero e Cura a Carattere Scientifico
2017
Policlinico San Matteo Fondazione
2017
University of Pavia
2017
We have developed an improved vector for the stable expression of recombinant protein in mammalian cells. In this vector, designated pCIN, both cDNA and neomycin phosphotransferase selection marker are transcribed from a single promoter element. To facilitate translation second open reading frame, encephalomyocarditis virus internal ribosome entry site has been inserted into cassette immediately before start codon sequence. report use to generate cell lines expressing human 5-HT1Da serotonin...
Homo- and hetero-oligomerization of G-protein-coupled receptors (GPCRs) were examined in HEK-293 cells using two variants bioluminescence resonance energy transfer (BRET). BRET2 (a variant BRET) offers greatly improved separation the emission spectra donor acceptor moieties compared with traditional BRET. Previously recorded homo-oligomerization human δ-opioid receptor was confirmed BRET2. Homo-oligomerization κ-opioid observed both BRET techniques. Both homo- hetero-oligomers, containing δ-...
Abstract Introduction Previous studies have shown through theoretical analyses that the ratio of partial pressure oxygen in arterial blood (PaO 2 ) to inspired fraction (FiO varies with FiO level. The aim present study was evaluate relevance this variation both theoretically and experimentally using mathematical model simulations, comparing these simulations PaO /FiO ratios measured a range different patients. Methods designed as retrospective data from 36 mechanically ventilated patients 57...
The human 5‐HT 5A serotonin receptor has been cloned. As with the mouse and rat receptors, gene consists of two coding exons separated by a large intron. deduced amino acid sequence reveals protein 357 residues which shares 93% (nucleotide) 84% (amino acid) identity to cloned receptor. We have determined tissue distribution reverse transcriptase‐PCR found expression in all regions brain examined little or no peripheral tissues. transiently expressed Cos M6 cells exhibits pharmacological...
Organ shortage is the major limitation to kidney transplantation in developed world. Conversely, millions of patients developing world with end-stage renal disease die because they cannot afford replacement therapy-even when willing living donors exist. This juxtaposition between countries funds but no available kidneys and those prompts us propose an exchange program using each nation's unique assets. Our proposal leverages cost savings achieved through earlier over dialysis fund...
Arterial oxygenation is often impaired during one-lung ventilation, due to both pulmonary shunt and atelectasis. The use of low tidal volume (VT) (5 ml/kg predicted body weight) in the context a lung-protective approach exacerbates This study sought determine combined physiologic effects positive end-expiratory pressure VT ventilation.Data from 41 patients studied general anesthesia for thoracic surgery were collected analyzed. Shunt fraction, high V/Q respiratory mechanics measured at 0 cm...
Several studies have found only a weak to moderate correlation between oxygenation and lung aeration in response changes PEEP. This study aimed investigate the association shunt, low high ventilation/perfusion (V/Q) mismatch, computed tomography-measured following an increase PEEP patients with ARDS.In this preliminary study, 12 ARDS were subjected recruitment maneuvers followed by setting at 5 then either 15 or 20 cmH2O. Lung was measured tomography. Values of pulmonary shunt V/Q mismatch...
To present a decision support system for optimising mechanical ventilation in patients residing the intensive care unit.Mathematical models of oxygen transport, carbon dioxide transport and lung mechanics are combined with penalty functions describing clinical preference toward goals side-effects theoretic approach. Penalties quantified risk barotrauma, acidosis or alkalosis, toxicity absorption atelectasis, hypoxaemia.The is presented an example its use post-surgical patient. The...
Apolipoprotein (apo) A-I is the principal protein component of plasma high density lipoproteins (HDLs). Tissue culture studies have suggested that lipid-free apo may, by recruiting phospholipids (PLs) and unesterified cholesterol from cell membranes, initiate reverse transport provide a nidus for formation, via lipid-poor, pre-β–migrating HDLs, spheroidal α-migrating HDLs. Apo has also been shown to inhibit hepatic lipase (HL) lipoprotein (LPL) in vitro. To further study its functions fate...
Introduction Advances in knowledge regarding mechanical ventilation (MV), particular lung-protective strategies, have been shown to reduce mortality. However, the translation of these advances into better therapeutic performance real-life clinical settings continues lag. High-fidelity simulation with a mannequin allows students interact lifelike situations; this may be valuable addition traditional didactic teaching. The purpose study is compare computer-based and mannequin-based approaches...
Cysteine351 is the site for pertussis toxin-catalyzed ADP-ribosylation in G protein Gi1α. Alteration of this residue, or equivalent cysteine other Gi-family proteins, has been used to examine specific interactions between receptors and these proteins. However, no systematic analysis performed determine quantitative effect such alterations. To address we mutated cysteine351 Gi1α all possible amino acids. Each mutants was transiently coexpressed along with porcine α2A-adrenoceptor HEK 293/T...
The α2A-adrenoceptor is the prototypic example of family G-protein-coupled receptors which function by activation ‘Gi-like’ pertussis toxin-sensitive G-proteins. A number members this subfamily G-proteins are often co-expressed in a single cell type. To examine interaction receptor with individual Gi-family porcine was transiently transfected into COS-7 cells either alone or each wild-type Gi1α, Gi2α and Gi3α mutations these cysteine residue target for toxin-catalysed ADP-ribosylation...
The human dopamine D<sub>2L</sub> receptor couples promiscuously to multiple members of the Gα<sub>i/o</sub> subfamily. Despite high homology and D<sub>3</sub> receptors, G protein coupling specificity is less clearly characterized. primary aim this study, then, was parallel characterization receptors. By using both receptor-G fusion proteins stable cell lines in which pertussis toxin-resistant mutants individual Gα<sub>i</sub>-family were expressed an inducible fashion, we demonstrated...
Clinical decision-making is based on knowledge, expertise, and authority, with clinicians approving almost every intervention-the starting point for delivery of "All the right care, but only care," an unachieved healthcare quality improvement goal. Unaided suffer from human cognitive limitations biases when decisions are their training, experience. Electronic health records (EHRs) could improve robust decision-support tools that reduce unwarranted variation clinician actions. Current EHRs,...