A5033774401- Circadian rhythm and melatonin
- Photoreceptor and optogenetics research
- Genetics, Aging, and Longevity in Model Organisms
- Sleep and Wakefulness Research
- Spaceflight effects on biology
- Neuroscience of respiration and sleep
- Gastroesophageal reflux and treatments
- Dietary Effects on Health
- Birth, Development, and Health
- Gastrointestinal motility and disorders
- Light effects on plants
- Helicobacter pylori-related gastroenterology studies
- Fatty Acid Research and Health
- Eosinophilic Esophagitis
- Drug Transport and Resistance Mechanisms
- Neuroendocrine regulation and behavior
- Neurobiology and Insect Physiology Research
- Diet and metabolism studies
- Prenatal Substance Exposure Effects
- Neuroscience and Neuropharmacology Research
- Stress Responses and Cortisol
- Protein Kinase Regulation and GTPase Signaling
- Climate Change and Health Impacts
- Potassium and Related Disorders
- Neurogenesis and neuroplasticity mechanisms
Texas A&M Health Science Center
2011-2025
Texas A&M University
2010-2022
University of Arizona
1991-2019
Novartis (China)
2015
San Francisco General Hospital
2015
University of California, San Francisco
2015
Prometheus (United States)
2014
Alcon (United States)
2004-2013
Novartis (United States)
2001-2013
Baylor Scott & White Health
2012
MicroRNAs (miRNAs) interact with 3′ untranslated region (UTR) elements of target genes to regulate mRNA stability or translation and thus play a role in regulating many different biological processes, including circadian rhythms. However, specific miRNAs mediating the regulation essential clock remain largely unknown. Because vesicles containing membrane-bound are present circulatory system, we examined predicted gene, Bmal1, for evidence rhythmic fluctuations circulating levels modulatory...
ABSTRACT The hormone melatonin phase shifts circadian rhythms generated by the mammalian biological clock, suprachiasmatic nucleus (SCN) of hypothalamus, through activation G protein‐coupled MT 2 receptors. This study demonstrated that pretreatment with physiological concentrations (30–300 pM or 7–70 pg/mL) decreased number hMT receptors heterologously expressed in cells a time and concentration‐dependent manner. Furthermore, ‐GFP immortalized SCN2.2 non‐neuronal were internalized upon both...
The master circadian pacemaker located within the suprachiasmatic nucleus (SCN) of mammalian brain controls system-level rhythms in animal physiology. Specific SCN outputs synchronize physiological other regions. Within SCN, communication among neural cells provides for coordination autonomous cellular oscillations into ensemble rhythms. ATP is a transmitter involved local astrocytes and between neurons. Using luciferin-luciferase chemiluminescence assay, we have demonstrated that levels...
Based on genetic models with mutation or deletion of core clock genes, circadian disruption has been implicated in the pathophysiology metabolic disorders. Thus, we examined whether desynchronization response to shift work-type schedules is sufficient compromise homeostasis and inflammatory mediators provide a key link mechanism by which alterations timekeeping contribute diet-induced dysregulation. In high-fat diet (HFD)-fed mice, exposure chronic shifts light-dark cycle (12 h advance every...
Cell lines derived from the rat suprachiasmatic nucleus (SCN) were screened for circadian clock properties distinctive of SCN in situ. Immortalized cells generated robust rhythms uptake metabolic marker 2-deoxyglucose and their content neurotrophins. The phase relationship between these vitro was identical to that exhibited by vivo. Transplantation cell lines, but not mesencephalic or fibroblast restored activity rhythm arrhythmic, SCN-lesioned rats. Thus, oscillator, pacemaker, are only...
The central pacemaker located in the suprachiasmatic nucleus (SCN) of hypothalamus mediates generation mammalian circadian rhythms, including an oscillation sensitivity to photic signals conveyed by retinohypothalamic tract. Because brain-derived neurotrophic factor (BDNF) has been implicated functional regulation neural input other targets visual pathways, present study examined whether changes BDNF expression or blockade its action SCN affect responses light. In rats receiving infusion...
A preponderance of carcinogenesis studies in rodents and epidemiologic humans suggests a potential role dietary fiber the prevention colorectal cancer. Recently, wheat bran used as supplement has been shown to decrease growth rectal adenomatous polyps patients with familial poluposis; however, few high-risk human populatins have attempted determine effects supplementation on markersn colon or rectum. We designed one-arm study evaluate [i.e., 13.5 g/day for 8 wk; after 1 mo, 2 (compliance...
The capacity to generate circadian rhythms endogenously and confer this rhythmicity other cells was compared in immortalized derived from the suprachiasmatic nucleus (SCN) a fibroblast line differentiate SCN pacemaker properties oscillatory behavior of non-clock tissues. Only SCN2.2 were capable generating 2-deoxyglucose uptake Per gene expression. Similar function vivo, imposed metabolic activity expression on cocultured NIH/3T3 fibroblasts via diffusible signal. conferred phase delayed by...
A pharmacological approach was used to examine the role of acetylcholine in photic control circadian rhythms and seasonal reproductive cycles. The experimental protocol designed determine whether administration carbachol, a cholinergic agonist, could mimic effects brief light pulses on gonadal function and/or rhythm wheel-running activity golden hamsters. Intraventricular injections administered singularly at discrete phase points throughout cycle, induced phase-dependent shifts free-running...
Individual neurons within the suprachiasmatic nuclei (SCNs) are capable of functioning as autonomous clocks and generating circadian rhythms in expression genes that form molecular clockworks. Limited information is available on how these oscillations individual clock cells coordinated to provide for ensemble rhythmicity normally observed from entire SCN. Because calcium influx via voltage-dependent channels (VDCCs) has been implicated regulation gene synchronization across population SCN...
The master circadian pacemaker located within the suprachiasmatic nuclei (SCN) controls neural and neuroendocrine rhythms in mammalian brain. Astrocytes are abundant SCN, this cell type displays clock gene expression extracellular accumulation of ATP. Still, intracellular signaling pathways that link SCN clockworks to ATP remain unclear. Because release from astrocytes is a calcium-dependent process, we investigated relationship between Ca<sup>2+</sup> have demonstrated levels fluctuate an...
A simple method for the gas–liquid chromatographic quantitation of human fecal bile acids and sterols is described where are subjected to n-butyl ester derivatization, without prior isolation from stool, followed by trimethylsilylation acids. Under these conditions, acid derivatives well resolved each other trimethylsilyl ether no overlap occurs. The was shown be highly reproducible recoveries were similar those obtained with methods used analysis. Application sterol analysis in stool...
MicroRNAs (miRNAs) are small non-coding RNAs that function as post-transcriptional modulators by regulating stability or translation of target mRNAs. Recent studies have implicated miRNAs in the regulation mammalian circadian rhythms. To explore role modulation core clock genes master pacemaker, we examined miR-142-3p for evidence expression suprachiasmatic nuclei (SCN), its putative gene Bmal1 via specific binding sites 3' UTR and overexpression-induced changes rhythm BMAL1 protein levels...
The present study was conducted to determine whether the photic induction of c-fos expression in rat suprachiasmatic nucleus (SCN) occurs within neurons containing gastrin-releasing peptide (GRP) and/or vasoactive intestinal polypeptide (VIP) because these peptidergic cells are closely associated with retinal projections ventrolateral subfield. Using dual immunostaining and thin sectioning techniques, SCN light-exposed rats examined for evidence individual coexpressing nuclear proteins (Fos)...
Abstract Mouse mammary epithelial cells (HC‐11) and tissues were analyzed for developmental changes in circadian clock, cellular proliferation, differentiation marker genes. Expression of the clock genes Per1 Bmal1 elevated differentiated HC‐11 cells, whereas Per2 mRNA levels higher undifferentiated cells. This differentiation‐dependent profile gene expression was consistent with that observed mouse glands, as late pregnant lactating tissues, proliferating virgin early glands. In both...