A5026231481- Neuroblastoma Research and Treatments
- MicroRNA in disease regulation
- Chromosomal and Genetic Variations
- RNA Interference and Gene Delivery
- 3D Printing in Biomedical Research
- Signaling Pathways in Disease
- Cellular Mechanics and Interactions
- Advanced biosensing and bioanalysis techniques
- RNA and protein synthesis mechanisms
- Epigenetics and DNA Methylation
- RNA modifications and cancer
- Cancer, Hypoxia, and Metabolism
- Lung Cancer Research Studies
- Cancer Cells and Metastasis
- CRISPR and Genetic Engineering
- Virus-based gene therapy research
- interferon and immune responses
- Viral Infectious Diseases and Gene Expression in Insects
- Immunotherapy and Immune Responses
- Cell death mechanisms and regulation
- Extracellular vesicles in disease
- Microtubule and mitosis dynamics
- Chemotherapy-induced cardiotoxicity and mitigation
- T-cell and Retrovirus Studies
- Silk-based biomaterials and applications
Advanced Materials and BioEngineering Research
2021-2024
Trinity College Dublin
2020-2024
Royal College of Surgeons in Ireland
2012-2022
National Children’s Research Centre
2012-2021
Children's Health Ireland at Crumlin
2015-2021
22q11 Ireland
2018
Dublin City University
2007-2013
G. K. Skryabin Institute of Biochemistry and Physiology of Microorganisms
2003-2006
Pushchino State Institute of Natural Sciences
1999-2000
Background Neuroblastoma is one of the most challenging malignancies childhood, being associated with highest death rate in paediatric oncology, underlining need for novel therapeutic approaches. Typically, patients high risk disease undergo an initial remission response to treatment, followed by recurrence that has become refractory further treatment. Here, we demonstrate first silica nanoparticle-based targeted delivery a tumor suppressive, pro-apoptotic microRNA, miR-34a, neuroblastoma...
Neuroblastoma is a challenging childhood malignancy, with very high percentage of patients relapsing following acquisition drug resistance, thereby necessitating the identification mechanisms resistance as well new biological targets contributing to aggressive pathogenicity disease. In order investigate molecular pathways that are involved in neuroblastoma, we have developed and characterised cisplatin resistant sublines SK-N-ASCis24, KellyCis83 CHP-212Cis100, integrating data cell...
Systemic lupus erythematosus (SLE) is a complex disease targeting multiple organs as result of overactivation the type I interferon (IFN) system, feature currently being targeted by biologic therapies against IFN-α. We have identified an estrogen-regulated microRNA, miR-302d, whose expression decreased in SLE patient monocytes and identify its target regulatory factor (IRF)-9, critical component transcriptional that regulates interferon-stimulated genes (ISGs). In keeping with reduced...
3D scaffold-based in vitro cell culturing is a recent technological advancement cancer research bridging the gap between conventional 2D culture and vivo tumours. The main challenge treating neuroblastoma, paediatric of sympathetic nervous system, to combat tumour metastasis resistance multiple chemotherapeutic drugs. aim this study was establish physiologically relevant neuroblastoma tissue-engineered system explore its therapeutic relevance. Two lines, sensitive Kelly resistant KellyCis83...
Current therapies for most non-infectious diseases are directed at or affect functionality of the human translated genome, barely 2% all genetic information. By contrast, therapeutic potential targeting transcriptome, ~ 70% remains largely unexplored. RNA therapeutics is an emerging field that widens range druggable targets and includes elements such as microRNA. Here, we sought to screen microRNA with tumor-suppressive functions in neuroblastoma, aggressive pediatric tumor sympathetic...
L1 elements (LINE‐1s) account for 17% of the human genome and have achieved this abundance by transpositions via an RNA intermediate, or retrotransposition. Reverse transcription is a crucial event in retrotransposition active element carried out L1‐encoded ORF2 protein. Previously, we performed biochemical characterization protein with reverse transcriptase (RT) activity (referred to as RT), expressed baculovirus‐infected insect cells. In present study, describe properties DNA‐...
The human LINE-1/L1 ORF2 protein is a multifunctional enzyme which plays vital role in the life cycle of L1 retrotransposon. consists an endonuclease domain, followed by central reverse transcriptase domain and carboxy-terminal C-domain with unknown function. Here, we explore nucleic acid binding properties 180-amino segment (CTS) ORF2p vitro. In series experiments involving gel shift assay, demonstrate that CTS binds RNA non-sequence-specific manner. Finally, report mutations destroying...
The acquisition of multidrug resistance is a major impediment to the successful treatment neuroblastoma, clinically heterogeneous cancer accounting for ∼15% all pediatric deaths. MYCN transcription factor, whose gene amplified in ∼30% high-risk neuroblastoma cases, influences drug by regulating cadre genes, including those involved with efflux, however, other subtypes lacking amplification, such as chromosome 11q deletions, also acquire resistance. To elucidate additional mechanisms non-MYCN...
// Aroa Soriano 1 , Laia París-Coderch Luz Jubierre Alba Martínez 2 Xiangyu Zhou 3 Olga Piskareva 4 Isabella Bray Isaac Vidal Ana Almazán-Moga Carla Molist Josep Roma José R. Bayascas Oriol Casanovas Raymond L. Stallings Sánchez de Toledo Soledad Gallego Miguel F. Segura Laboratory of Translational Research in Child and Adolescent Cancer, Vall d'Hebron Institute (VHIR)-UAB, Barcelona, Spain Tumor Angiogenesis Group, Catalan Oncology-IDIBELL, L'Hospitalet Llobregat, Institut Neurociències...
Neuroblastoma (NB) is a neural crest-derived tumor, which develops before birth or in early childhood, with metastatic dissemination typically preceding diagnosis. Tumors are characterized by highly heterogeneous combination of cellular phenotypes demonstrating varying degrees differentiation along different lineage pathways, and possessing distinct super-enhancers core regulatory circuits, thereby leading to varied malignant potential divergent clinical outcomes. Cytoskeletal reorganization...
Neuroblastoma is a paediatric malignancy of the developing sympathetic nervous system. About half patients have metastatic disease at time diagnosis and survival rate less than 50%. Our understanding cellular processes promoting neuroblastoma metastases will be facilitated by development appropriate experimental models. In this study, we aimed to explore invasion cells organoids from patient-derived xenografts (PDXs) grown embedded in 3D extracellular matrix (ECM) hydrogels time-lapse...
The recognition of the biological, diagnostic and medical importance exosomes has given rise to an urgent need for efficient labelling these extracellular vesicles in ways that do not alter their inherent characteristics. We report first time endogenous method NIR-fluorescent labelled using amphiphilic probe without immunolabelling or synthetic chromatographic manipulation exosomes. Comparative analyses unlabelled with NTA, AFM, flow cytometry immunoblot analysis all show a high degree...
Neuroblastoma is the most common solid extracranial tumour in children. Despite major advances available therapies, children with drug-resistant and/or recurrent neuroblastoma have a dismal outlook 5-year survival rates of less than 20%. Therefore, tackling relapsed biology by developing and characterising clinically relevant models priority finding targetable vulnerability neuroblastoma. Using matched cisplatin-sensitive KellyLuc resistant KellyCis83Luc cell lines, we developed...
Neuroblastoma is the most common extracranial solid tumor in children, accounting for 15% of overall pediatric cancer deaths. The native tissue a complex three-dimensional (3D) microenvironment involving layers cancerous and non-cancerous cells surrounded by an extracellular matrix (ECM). ECM provides physical biological support contributes to disease progression, patient prognosis, therapeutic response. This paper describes protocol assembling 3D scaffold-based system mimic neuroblastoma...