Paleo-subsurface rivers can serve as high-quality reservoir spaces for hydrocarbon accumulation within the carbonate sequence stratigraphy. However, characteristics and evolutionary process of typical low-mature paleo-subsurface are rarely discussed, constraining petroleum exploration exploitation. This study employs an integrated approach, combining seismic-geological analysis, core thin sections, logging data, production data to investigate structural features processes river in Halahatang...

The avian leukosis virus subgroup J (ALV-J) belongs to the chicken retrovirus that causes enormous economic losses in poultry industry. Interferon-stimulated genes (ISGs) are critical for controlling infections. Here, we identified 897 type I ISGs induced by interferon-α (IFN-α) peripheral blood mononuclear cell (PBMC) RNA-Seq. In addition, further 152 potential anti-ALV-J ISGs. Among these ISGs, cholesterol 25-hydroxylase (

Autophagy plays an important role in host antiviral defense. The avian leukosis virus subgroup J (ALV-J) has been shown to inhibit autophagy while promoting viral replication. underlying autophagic mechanisms, however, are unknown. Cholesterol 25-hydroxylase (CH25H) is a conserved interferon-stimulated gene, which converts cholesterol soluble factor, 25-hydroxycholesterol (25HC). In this study, we further investigated the mechanism of CH25H resistance ALV-J chicken embryonic fibroblast cell...

Avian leukosis virus Subgroup J (ALV-J) exhibits high morbidity and pathogenicity, affecting approximately 20% of poultry farms. It induces neoplastic diseases immunosuppression. Phorbol-12-myristate-13-acetate-induced protein 1 (PMAIP1), a pro-apoptotic mitochondrial in the B-cell lymphoma-2 (Bcl-2) family, plays role apoptosis cancer cells. However, connection between PMAIP1 gene ALV-J pathogenicity remains unexplored. This study investigates potential impact on replication its regulatory...

The accumulation of abdominal fat and the metabolic dysfunction-associated fatty liver disease (MAFLD) are prevalent problems in poultry industry, seriously compromise broiler health reduce economic benefits. Echinocystic acid (EA), a natural product with anti-inflammatory antioxidant effects, has been demonstrated to deposition improve intestinal inflammation mice. However, it not reported research. In this study, we employed chicken hepatocytes (Leghorn male hepatoma cells, LMHs) construct...

Avian leukosis virus subgroup J (ALV-J) is an oncogenic retrovirus that causes immunosuppression and neoplastic diseases in poultry. Cytokine signal-transduction inhibitor molecule 3 (SOCS3) important negative regulator of the JAK2/STAT3 signaling pathway plays certain roles ALV-J infection. It significance to confirm SOCS3 infection study how this gene affects In study, we assessed expression vivo vitro , investigated using overexpressed or interfered assays with DF-1 cells. The results...

J subgroup avian leukosis virus (ALV-J) infection causes serious immunosuppression problems, leading to hematopoietic malignancy tumors in chicken. It has been demonstrated that interferon-stimulated genes (ISGs) could limit ALV-J replication; nevertheless, the underlying mechanisms remain obscure. Here, we demonstrate Long-chain Acyl-CoA synthetase 1 (ACSL1) is an interferon (IFN)-stimulated gene specifically restricts replication of due higher IFN-I production. More importantly, ACSL1...

Golgin subfamily B member 1 (GOLGB1) gene encodes the coat protein vesicle inhibiting factor, giantin. Previous study showed that mutations of GOLGB1 are associated with dozens human developmental disorders and diseases. However, biological function in chicken is still unclear. In this study, we detected a novel 65-bp insertion/deletion (indel) polymorphism intron 5. Association indel growth carcass traits was analyzed yellow population. Results significantly body weight (p < 0.05), highly...

Myoblast differentiation requires metabolic reprogramming driven by increased mitochondrial biogenesis and oxidative phosphorylation. The canonical GH-GHR-IGFs axis in liver exhibits a great complexity response to somatic growth. However, the underlying mechanism of whether local GHR acts as control valve regulate function through during myoblast remains unknown.We manipulated expression chicken primary investigate its roles differentiation.We reported that is induced differentiation. Local...

To understand the differences in immune responses between early feathering (EF) and late (LF) chickens after infection with avian leukosis virus, subgroup J (ALV-J), we monitored levels of prolactin, growth hormone immunoglobulins IgG IgM serum LF EF for 8 weeks. Moreover, analysed expression immune-related genes spleen PRLR, SPEF2 dPRLR organs DF-1 cells by qRT-PCR. The results showed that ALV-J affected hormone, serum. Regardless whether were infected ALV-J, two hormones higher than those...

The birth weight of chickens does not significantly affect the at slaughter, while different growth rate after was one important reasons for difference in slaughter weight. Also, increase chickens' postnatal skeletal muscle is main cause gain, but which genes are involved this biological process still unclear. In study, by integrating four transcriptome datasets containing chicken muscles developmental times or tissues public databases, a total nine candidate that may be related to...

The aim of this study was to better understand the sequence characteristics and immune responses in avian leukosis virus subgroup J (ALV-J) infected yellow chicken flocks South China. We isolated four strains ALV-J from these flocks, which were then identified by several methods, including subtype-specific polymerase chain reaction (PCR), enzyme-linked immunosorbent assay (ELISA), immunofluorescence (IFA). All viruses sequenced for their complete genomes named GD19GZ01, GD19GZ02, GD19GZ03,...

Growth hormone (GH) plays a crucial role in growth, sexual maturity, and immunity chickens. Avian leukosis virus subgroup J (ALV-J) is an exogenous tumorigenic retrovirus that primarily induces immunosuppression, growth retardation, decreased egg production, tumors formation, even death Previous studies have suggested GH involved the regulation of innate inflammation. However, specific response to ALV-J remains unclear. In this study, we observed significant upregulation protein expression...

Summary Parathyroid hormone ( PTH ) is a crucial regulator of calcium homeostasis and bone remodeling, the parathyroid 1 receptor PTH1R belongs to class II G‐protein‐coupled receptor. activates , which mediates catabolic anabolic processes in skeleton. However, functional mechanism has not been thoroughly elucidated organisms. This study identified 51 bp indel mutation first intron gene effect this on growth carcass traits chickens. The results indicated that was significantly associated...

Abstract Background G-protein subunit beta 1 like ( GNB1L ) encodes a beta-subunit-like polypeptide. Chicken is upregulated in the breast muscle of high feed efficiency chickens, and its expression 1.52-fold that low chickens. However, no report has described effects indels on chicken carcass growth traits. Results This study identified 31-bp indel 5′ untranslated region (UTR) elucidated effect this gene mutation traits The showed highly significant association with body weight at 8...

Endogenous retroviruses (ERVs) are viral sequences that have integrated into the genomes of vertebrates. Our preliminary transcriptome sequencing analysis revealed chERV3 is active and located on chromosome 1:32602284–32615631. We hypothesized may a role in host innate immune response to infection. In this study, using reverse genetics, we constructed puc57-chERV3 full-length cloning plasmid vitro. measured p27 content culture supernatant by enzyme-linked immunosorbent assay (ELISA)....