A5090730406- Ubiquitin and proteasome pathways
- Bone fractures and treatments
- Protein Degradation and Inhibitors
- Orthopedic Surgery and Rehabilitation
- Immunotherapy and Immune Responses
- interferon and immune responses
- Cancer-related Molecular Pathways
- RNA Interference and Gene Delivery
- Autophagy in Disease and Therapy
- Cell death mechanisms and regulation
- Hip and Femur Fractures
- X-ray Diffraction in Crystallography
- Peptidase Inhibition and Analysis
- RNA modifications and cancer
- Elbow and Forearm Trauma Treatment
- Crystallization and Solubility Studies
- Multiple Myeloma Research and Treatments
- Artificial Intelligence in Healthcare and Education
- Metalloenzymes and iron-sulfur proteins
- Osteomyelitis and Bone Disorders Research
- Sirtuins and Resveratrol in Medicine
- Microtubule and mitosis dynamics
- HER2/EGFR in Cancer Research
- Breast Cancer Treatment Studies
- Tracheal and airway disorders
Amgen (United States)
2022-2025
St. Jude Children's Research Hospital
1993-2021
University of Ulsan
2019
Asan Medical Center
2019
Ulsan College
2019
Seoul National University
2010-2016
University of Florida Health
2011
UF Health Cancer Center
2011
Maryknoll Medical Center
1994-1997
Abstract p53 plays a pivotal role in tumour suppression under stresses, such as DNA damage. ISG15 has been implicated the control of tumorigenesis. Intriguingly, expression ISG15, UBE1L and UBCH8 is induced by DNA-damaging agents, ultraviolet doxorubicin, which are known to induce p53. Here, we show that genes encoding UBE1L, EFP, have p53-responsive elements their p53-dependent fashion damage conditions. Furthermore, induces conjugation this modification markedly enhances binding promoters...
Targeting cereblon (CRBN) is currently one of the most frequently reported proteolysis-targeting chimera (PROTAC) approaches, owing to favorable drug-like properties CRBN ligands, immunomodulatory imide drugs (IMiDs). However, IMiDs are known be inherently unstable, readily undergoing hydrolysis in body fluids. Here we show that and IMiD-based PROTACs rapidly hydrolyze commonly utilized cell media, which significantly affects their efficacy. We designed novel binders, phenyl glutarimide (PG)...
Katanin is a heterodimeric enzyme that severs and disassembles microtubules. While the p60 subunit has activity, p80 regulates activity. The microtubule-severing activity of katanin plays an essential role in axonal growth. However, mechanisms by which neuronal cells regulate expression katanin-p60 remains unknown. Here we showed USP47 C terminus Hsp70-interacting protein (CHIP) antagonistically stability thereby was identified as katanin-p60-specific deubiquitinating for its stabilization....
Abstract Testis-restricted melanoma antigen (MAGE) proteins are frequently hijacked in cancer and play a critical role tumorigenesis. MAGEs assemble with E3 ubiquitin ligases function as substrate adaptors that direct the ubiquitination of novel targets, including key tumor suppressors. However, how recognize their targets is unknown has impeded development MAGE-directed therapeutics. Here, we report structural basis for recognition by MAGE ligases. Biochemical analysis degron motif...
Abstract Targeting cereblon (CRBN) is currently one of the most frequently reported proteolysis‐targeting chimera (PROTAC) approaches, owing to favorable drug‐like properties CRBN ligands, immunomodulatory imide drugs (IMiDs). However, IMiDs are known be inherently unstable, readily undergoing hydrolysis in body fluids. Here we show that and IMiD‐based PROTACs rapidly hydrolyze commonly utilized cell media, which significantly affects their efficacy. We designed novel binders, phenyl...
The encephalitogenic potential of a segment myelin basic protein in experimental autoimmune encephalomyelitis is not always mirrored by the ability peptide to mediate vitro activation T cells. Recent studies from our laboratory have demonstrated that responsiveness Ag-specific cells determined exclusively Ag but also nature APC. By varying APC during selection cells, we could generate distinct sets rat as evidenced diversity TCR usage. Here establish importance peptides. Peptides 69-84-Gly...
c-Cbl, a RING-type ubiquitin E3 ligase, downregulates various receptor tyrosine kinases (e.g., epidermal growth factor (EGFR)), leading to inhibition of cell proliferation. Moreover, patients with myeloid neoplasm frequently harbor c-Cbl mutations, implicating the role as tumor suppressor. Recently, we have shown that αPix-mediated migration and invasion, lack in rat C6 human A172 glioma cells is responsible for their malignant behavior. Here, showed exon skipping occurs brain tissues from...
Nutrient-responsive protein kinases control the balance between anabolic growth and catabolic processes such as autophagy. Aberrant regulation of these is a major cause human disease. We report here that vertebrate nonreceptor tyrosine kinase Src-related lacking C-terminal regulatory N-terminal myristylation sites (SRMS) inhibits autophagy promotes in nutrient-responsive manner. Under nutrient-replete conditions, SRMS phosphorylates PHLPP scaffold FK506-binding 51 (FKBP51), disrupts...
The prognosis of hormone receptor-positive and HER2-negative breast cancer is better than that other subtypes. Current guidelines recommend chemotherapy for N1 patients. However, this has the possibility to be over-treatment.This was a retrospective study 18,549 patients who were surgically treated invasive cancer, at single center in South Korea, between January 1993 December 2012. stage enrolled, propensity score matching performed compare with anti-hormonal therapy alone (N = 83) those...
ABSTRACT In targeted protein degradation (TPD) a of interest is degraded by chemically induced proximity to an E3 ubiquitin ligase. One limitation using TPD therapeutically that most ligases have broad tissue expression, which can contribute toxicity via target in healthy cells. Many pathogenic and oncogenic viruses encode (vE3s), de facto strictly limited expression diseased Here, we provide proof-of-concept for Vi ral P an- E ssential R emoving Ta rgeting C himeras (VIPER-TACs) are...
Under normal conditions, apoptosis inducing factor (AIF) in the mitochondrial membrane functions as an oxidoreductase that scavenges reactive oxygen species. certain stresses, such exposure to N ‐methyl‐ N′ ‐nitro‐ ‐nitrosoguanidine (MNNG), AIF is truncated from mitochondria and translocated nucleus, where it induces caspase‐independent programmed cell death, featured by chromatin condensation large‐scale DNA breakage. Here we demonstrate USP2 deubiquitinates whereas CHIP ubiquitin E3 ligase...